Native Adenovirus Type 5 hexon protein is a Human adenovirus 5 Full Length protein, in the 2 to 952 aa range, expressed in Native, with >95% purity and suitable for SDS-PAGE, ELISA.
Images
Publications
Filter by
- Science immunology 6:eabi86352021Fractionating a COVID-19 Ad5-vectored vaccine improves virus-specific immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Sanchez,Nicole Palacio,Tanushree Dangi,Thomas Ciucci,Pablo Penaloza-MacMasterPubMed 34648369
- Journal of virology 87:1893-82012Amino acid exchanges in the putative nuclear export signal of adenovirus type 5 L4-100K severely reduce viral progeny due to effects on hexon biogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOrkide O Koyuncu,Thomas Speiseder,Thomas Dobner,Melanie SchmidPubMed 23175361
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Native
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Major capsid protein that self-associates to form 240 hexon trimers, each in the shape of a hexagon, building most of the pseudo T=25 capsid. Assembled into trimeric units with the help of the chaperone shutoff protein. Transported by pre-protein VI to the nucleus where it associates with other structural proteins to form an empty capsid. Might be involved, through its interaction with host dyneins, in the intracellular microtubule-dependent transport of incoming viral capsid to the nucleus.
Additional Targets
Adenovirus Type 5 hexon
Alternative names
Hexon protein, CP-H, Protein II, L3
Recommended products
Native Adenovirus Type 5 hexon protein is a Human adenovirus 5 Full Length protein, in the 2 to 952 aa range, expressed in Native, with >95% purity and suitable for SDS-PAGE, ELISA.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Native
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA
- Biologically active
- No
- Accession
- P04133-1
- Animal free
- No
- Species
- Human adenovirus 5
- Concentration
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 2.19% Sodium chloride, 0.28% Bis-Tris propane
Sequence info
Amino acid sequence
- Accession
- P04133
- Protein length
- Full Length
- Predicted molecular weight
- 108 kDa
- Amino acids
- 2 to 952
- Nature
- Native
Specifications
- Form
- Liquid
- Additional notes
Purification involves multiple steps including sequential size exclusion and ion exchange chromatography.
General info
- Function
Major capsid protein that self-associates to form 240 hexon trimers, each in the shape of a hexagon, building most of the pseudo T=25 capsid. Assembled into trimeric units with the help of the chaperone shutoff protein. Transported by pre-protein VI to the nucleus where it associates with other structural proteins to form an empty capsid. Might be involved, through its interaction with host dyneins, in the intracellular microtubule-dependent transport of incoming viral capsid to the nucleus.
- Sequence similarities
Belongs to the adenoviridae hexon protein family.
- Subcellular localisation
- Host nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Hexon protein naturally self assembles as part of the virus life cycle. When the protein is purified we also see evidence of self assembly such as rapid loss of protein upon serial dilution. The storage buffer and ELISA coating buffer play an important role in the likelihood of self assembly and thereby can affect results when using purified hexon as antigen in ELISAs and other such assays. Many common ELISA coating buffers increase self assembly in hexon protein as they are designed to increase efficiency of antigen adsorption. This results in a very high signal with the initial dilution but then the sensitivity tails off very rapidly. Diluting and coating the hexon in water alone slightly increases sensitivity but reduces strength; coating and diluting in the presence of a small amount of stabilising protein (such as BSA) seems to increase sensitivity for antigen coating significantly (although too much BSA can block high concentrations of hexon).
This protein is derived from a pathogenic organism, and may be involved in a disease process, consequently exposure may have adverse health effects.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Adenovirus Type 5 hexon also known as hexon protein or 5 protein is a major structural component of adenovirus capsid with a mass of approximately 960 kDa. Hexon proteins form the outer shell of the virus giving it stability and shape. These proteins are heavily expressed on the surface of human adenovirus serotype 5 a common virus strain used in therapeutic vector development. Hexon comprises three regions: the hypervariable regions conserved regions and the base that interacts with other capsid proteins.
- Biological function summary
Adenovirus hexon proteins participate in the assembly and stability of the virus capsid complex. The hexon proteins interact with other components like penton base and fiber proteins to form a robust and protective viral coat. Their function involves binding to cell surface receptors such as the coxsackievirus and adenovirus receptor (CAR) allowing the virus to attach to and enter host cells. This interaction is key in viral infections and also in viral vector efficiency.
- Pathways
The adenovirus Type 5 hexon protein plays important roles in immune evasion and viral entry pathways. The hexon protein helps the virus escape host immune detection by eluding neutralizing antibodies. The internal pathways also involve interactions with proteins like E1A which modulates host cell cycle pathways for viral replication. These interactions integrate hexon proteins into the broader adenoviral life cycle and infection processes.
- Associated diseases and disorders
Infections involving adenovirus Type 5 relate to respiratory illnesses and conjunctivitis. Respiratory infections often occur in crowded environments such as schools and military barracks and the hexon protein's interaction with the immune system can exacerbate symptoms. Additionally due to its prevalent role in viral vector design research on hexon relates to gene therapy contexts scrutinizing how hexon modifications impact disease treatment and host immune response. Hemagglutinin might connect to respiratory disorders linked to adenovirus making it also relevant in developing therapies.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Native Adenovirus Type 5 hexon protein (ab123995)
Lane 1: Ladder
Lane 2: Ad5
Lane 3: Ad5 crude prep
Lane 4: Ad5 prep after filtration
Lane 5: Hybrid purified Hexon
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com