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AB136212

Native Chicken Deglycosylated Avidin protein (HRP)

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Native Chicken Deglycosylated Avidin protein (HRP) is a Chicken Full Length protein, in the 1 to 152 aa range, expressed in Native, with >90%, suitable for WB, Flow Cyt, ELISA.

View Alternative Names

Avidin, AVD

Key facts

Purity

>90% SDS-PAGE

Expression system

Native

Tags

Tag free

Applications

ELISA, WB, Flow Cyt

applications

Biologically active

No

Conjugation

HRP

Excitation/Emission
Accession

P02701

Animal free

No

Carrier free

No

Species

Chicken

Storage buffer

pH: 7.4 Constituents: 50% PBS, 49% Glycerol (glycerin, glycerine)

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/4000 - 1/8000", "notes":"<p></p>" } } }

Product details

Deglycosylated acidic avidin has higher affinity for biotin than standard avidin and therefore reduces background staining. ab136212 reacts with D-Biotin.

Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"60 kDa","actualMolecularWeight":null,"aminoAcidEnd":152,"aminoAcidStart":1,"nature":"Native","expressionSystem":null,"accessionNumber":"P02701","tags":[]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Store in the dark
False

Specifications

Form

Liquid

Additional notes

Purification methods are proprietary, using a combination of precipitation and/or chromatography techniques. Purification percentages provided relate prior to conjugation.

General info

Function

The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).

Sequence similarities

Belongs to the avidin/streptavidin family.

Post-translational modifications

N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc.

Product protocols

Target data

The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
See full target information Avidin

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