Native Chlamydia trachomatis protein is a Full Length protein and expressed in Native.
Images
Key facts
- Expression system
- Native
- Tags
- Tag free
- Biologically active
- No
Alternative names
C.trachomatis
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Native Chlamydia trachomatis protein is a Full Length protein and expressed in Native.
Key facts
- Expression system
- Native
- Tags
- Tag free
- Biologically active
- No
- Animal free
- No
- Concentration
- Storage buffer
pH: 7 - 8
Constituents: 99% PBS, 0.05% Triton X-100
Specifications
- Form
- Liquid
- Additional notes
Chlamydia trachomatis is propagated to maximum density in McCoy cells under Category 2 safety conditions. Infected cells are carefully washed and disrupted into buffered saline (PBS), subjected to 3 freeze/thaw cycles and centrifuged to remove cellular debris. Saline lysate is further purified by sucrose gradient and ultracentrifugation to yield Chlamydia of a much higher purity. Inactivation is by heat treatment and the addition of triton x-100 to render products non-infectious and improve stability. All preparations are tested as no infectious by double passage on McCoy cell under optimal growth conditions.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Inactivation: Heat treatment and 0.05% Triton x-100. Tested inactive by two blind passages on permissive cells showing no signs of inclusion bodies.
This protein is derived from a pathogenic organism, and may be involved in a disease process, consequently exposure may have adverse health effects.
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Native Chlamydia trachomatis protein (ab123998)
ab123998 shows fixed and stained McCoy cells infected with Chlamydia trachomatis
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