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AB64009

Native Cow Bovine Serum Albumin protein

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(8 Publications)

Native Cow Bovine Serum Albumin protein is a Cow Full Length protein, in the 25 to 607 aa range, expressed in Native, with >96%, suitable for WB, IHC.

View Alternative Names

Albumin, BSA, ALB

Key facts

Purity

>96% SDS-PAGE

Expression system

Native

Tags

Tag free

Applications

IHC, WB

applications

Biologically active

No

Accession

P02769

Animal free

No

Carrier free

No

Species

Cow

Reconstitution

Reconstitute using deionized water.

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Complementary Products

Reagents, Controls & Accessories

AB116027

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB116027)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB207327

Anti-Albumin antibody [EPR20195]

RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (AB207327)

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  • 2
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

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Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The product is suitable to use in blocking experiments. This product contains 1.4%Ash: 1.4%Heavy Metals: <0.44ppmBovine IgG: Not detectedProtein: 100% (Biuret)Albumin: 100% (electrophoresis)
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Sequence info

[{"sequence":"DTHKSEIAHRFKDLGEEHFKGLVLIAFSQYLQQCPFDEHVKLVNELTEFAKTCVADESHAGCEKSLHTLFGDELCKVASLRETYGDMADCCEKQEPERNECFLSHKDDSPDLPKLKPDPNTLCDEFKADEKKFWGKYLYEIARRHPYFYAPELLYYANKYNGVFQECCQAEDKGACLLPKIETMREKVLASSARQRLRCASIQKFGERALKAWSVARLSQKFPKAEFVEVTKLVTDLTKVHKECCHGDLLECADDRADLAKYICDNQDTISSKLKECCDKPLLEKSHCIAEVEKDAIPENLPPLTADFAEDKDVCKNYQEAKDAFLGSFLYEYSRRHPEYAVSVLLRLAKEYEATLEECCAKDDPHACYSTVFDKLKHLVDEPQNLIKQNCDQFEKLGEYGFQNALIVRYTRKVPQVSTPTLVEVSRSLGKVGTRCCTKPESERMPCTEDYLSLILNRLCVLHEKTPVSEKVTKCCTESLVNRRPCFSALTPDETYVPKAFDEKLFTFHADICTLPDTEKQIKKQTALVELLKHKPKATEEQLKTVMENFVAFVDKCCAADDKEACFAVEGPKLVVSTQTALA","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":607,"aminoAcidStart":25,"nature":"Native","expressionSystem":"Native","accessionNumber":"P02769","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bovine serum albumin (BSA) also known as albumin bovine serum boasts a molecular weight of approximately 66.5 kDa. This protein is found in the blood serum of cows and is derived from plasma. BSA or bovine albumin acts as a transport protein that binds to various substances including fatty acids and hormones facilitating their movement through the blood. In the lab scientists often use the BSA protein as a standard or blocking agent in experiments particularly in ELISA and Western blot assays.
Biological function summary

Proteins such as BSA play a critical role in maintaining osmotic pressure and pH balance in biological fluids. BSA does not form part of a larger protein complex but interacts with numerous small molecules and ions stabilizing their concentrations in the bloodstream. The interaction of albumin bovine serum with fatty acids is especially important for modulating the bioavailability of lipophilic molecules.

Pathways

Albumin actively contributes to lipid metabolism and transport pathways. It interacts closely with other proteins involved in these processes such as lipoproteins. BSA's binding affinity for various drugs and toxins also places it within the detoxification and excretory pathways ensuring the stability and solubility of these substances in circulation.

Scientists have studied BSA for its relevance to liver and kidney diseases given its role in carrying molecules and maintaining osmotic balance. Reduced levels of bovine albumin may indicate liver dysfunction or nephrotic syndrome where protein loss through urine is a concern. BSA's interaction with proteins such as globulin is also significant when investigating these conditions as the balance between these proteins can reflect the body's overall protein status.
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Specifications

Form

Lyophilized

Additional notes

Proprietary heat-shock process.

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General info

Function

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (Probable). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (PubMed : 22677715). The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (Probable). The rank order of affinity is zinc > calcium > magnesium (Probable). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed : 6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed : 6234017).

Sequence similarities

Belongs to the ALB/AFP/VDB family.

Post-translational modifications

Phosphorylated by FAM20C in the extracellular medium.

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Product protocols

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Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (Probable). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (PubMed : 22677715). The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (Probable). The rank order of affinity is zinc > calcium > magnesium (Probable). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed : 6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed : 6234017).
See full target information Albumin
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 5:103444 PubMed39514390

2024

A protocol for immunostaining of macrophages in whole-mount mouse cornea, conjunctiva, and lacrimal gland.

Applications

Unspecified application

Species

Unspecified reactive species

Saleh Alfuraih,Dhruv Patel,Wonsuk Choi,Karthikeyan Ramasamy,Rais Ansari,Ajay Sharma

Molecular medicine reports 30: PubMed39329201

2024

Exosomal miR‑194 from adipose‑derived stem cells impedes hypertrophic scar formation through targeting TGF‑β1.

Applications

Unspecified application

Species

Unspecified reactive species

Zhishan Xu,Yuan Tian,Lijun Hao

Nature communications 15:6774 PubMed39117721

2024

In situ assembly of an injectable cardiac stimulator.

Applications

Unspecified application

Species

Unspecified reactive species

Umut Aydemir,Abdelrazek H Mousa,Cedric Dicko,Xenofon Strakosas,Muhammad Anwar Shameem,Karin Hellman,Amit Singh Yadav,Peter Ekström,Damien Hughes,Fredrik Ek,Magnus Berggren,Anders Arner,Martin Hjort,Roger Olsson

Pharmaceutical biology 60:1407-1416 PubMed35938471

2022

Baicalin protects against renal interstitial fibrosis in mice by inhibiting the TGF-β/Smad signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Wang,Qingtao Jiang,Lizhu Zhang

Analytical chemistry 93:14527-14536 PubMed34668374

2021

Depletion of Highly Abundant Protein Species from Biosamples by the Use of a Branched Silicon Nanopillar On-Chip Platform.

Applications

Unspecified application

Species

Unspecified reactive species

Ella Borberg,Sofiya Pashko,Vlad Koren,Larisa Burstein,Fernando Patolsky

Experimental and therapeutic medicine 21:469 PubMed33767764

2021

Effects of sevoflurane general anesthesia during early pregnancy on AIM2 expression in the hippocampus and parietal cortex of Sprague-Dawley offspring rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yulin Zhu,Chao Lv,Jingying Liu,Shujun Shang,Wei Jing

Molecular medicine reports 23: PubMed33576465

2021

Prenatal ethanol exposure impairs the formation of radial glial fibers and promotes the transformation of GFAPδ‑positive radial glial cells into astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Li,Li-Na Zhang,Li Chong,Yue Liu,Feng-Yu Xi,Hong Zhang,Xiang-Long Duan

Physiological research 68:219-231 PubMed30628831

2019

Downregulation of endothelial transient receptor potential vanilloid type 4 channel underlines impaired endothelial nitric oxide-mediated relaxation in the mesenteric arteries of hypertensive rats.

Applications

Unspecified application

Species

Unspecified reactive species

A Boudaka,M Al-Suleimani,I Al-Lawati,H Baomar,S Al-Siyabi,F Zadjali
View all publications
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