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Native Cow Bovine Serum Albumin protein is a Cow Full Length protein, in the 25 to 607 aa range, expressed in Native, with >96% purity and suitable for WB, IHC.

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Key facts

Purity

>96% SDS-PAGE

Expression system

Native

Tags

Tag free

Applications

WB, IHC

Biologically active

No

Amino acid sequence

D T H K S E I A H R F K D L G E E H F K G L V L I A F S Q Y L Q Q C P F D E H V K L V N E L T E F A K T C V A D E S H A G C E K S L H T L F G D E L C K V A S L R E T Y G D M A D C C E K Q E P E R N E C F L S H K D D S P D L P K L K P D P N T L C D E F K A D E K K F W G K Y L Y E I A R R H P Y F Y A P E L L Y Y A N K Y N G V F Q E C C Q A E D K G A C L L P K I E T M R E K V L A S S A R Q R L R C A S I Q K F G E R A L K A W S V A R L S Q K F P K A E F V E V T K L V T D L T K V H K E C C H G D L L E C A D D R A D L A K Y I C D N Q D T I S S K L K E C C D K P L L E K S H C I A E V E K D A I P E N L P P L T A D F A E D K D V C K N Y Q E A K D A F L G S F L Y E Y S R R H P E Y A V S V L L R L A K E Y E A T L E E C C A K D D P H A C Y S T V F D K L K H L V D E P Q N L I K Q N C D Q F E K L G E Y G F Q N A L I V R Y T R K V P Q V S T P T L V E V S R S L G K V G T R C C T K P E S E R M P C T E D Y L S L I L N R L C V L H E K T P V S E K V T K C C T E S L V N R R P C F S A L T P D E T Y V P K A F D E K L F T F H A D I C T L P D T E K Q I K K Q T A L V E L L K H K P K A T E E Q L K T V M E N F V A F V D K C C A A D D K E A C F A V E G P K L V V S T Q T A L A

Reactivity data

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

-

Application

IHC

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (Probable). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (PubMed:22677715). The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (Probable). The rank order of affinity is zinc > calcium > magnesium (Probable). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).

Alternative names

Recommended products

Native Cow Bovine Serum Albumin protein is a Cow Full Length protein, in the 25 to 607 aa range, expressed in Native, with >96% purity and suitable for WB, IHC.

Key facts

Purity

>96% SDS-PAGE

Expression system

Native

Applications

WB, IHC

Accession
P02769-1
Animal free

No

Species

Cow

Reconstitution

Reconstitute using deionized water.

Concentration
Loading...

Sequence info

Amino acid sequence

D T H K S E I A H R F K D L G E E H F K G L V L I A F S Q Y L Q Q C P F D E H V K L V N E L T E F A K T C V A D E S H A G C E K S L H T L F G D E L C K V A S L R E T Y G D M A D C C E K Q E P E R N E C F L S H K D D S P D L P K L K P D P N T L C D E F K A D E K K F W G K Y L Y E I A R R H P Y F Y A P E L L Y Y A N K Y N G V F Q E C C Q A E D K G A C L L P K I E T M R E K V L A S S A R Q R L R C A S I Q K F G E R A L K A W S V A R L S Q K F P K A E F V E V T K L V T D L T K V H K E C C H G D L L E C A D D R A D L A K Y I C D N Q D T I S S K L K E C C D K P L L E K S H C I A E V E K D A I P E N L P P L T A D F A E D K D V C K N Y Q E A K D A F L G S F L Y E Y S R R H P E Y A V S V L L R L A K E Y E A T L E E C C A K D D P H A C Y S T V F D K L K H L V D E P Q N L I K Q N C D Q F E K L G E Y G F Q N A L I V R Y T R K V P Q V S T P T L V E V S R S L G K V G T R C C T K P E S E R M P C T E D Y L S L I L N R L C V L H E K T P V S E K V T K C C T E S L V N R R P C F S A L T P D E T Y V P K A F D E K L F T F H A D I C T L P D T E K Q I K K Q T A L V E L L K H K P K A T E E Q L K T V M E N F V A F V D K C C A A D D K E A C F A V E G P K L V V S T Q T A L A

Accession

P02769

Protein length

Full Length

Amino acids

25 to 607

Nature

Native

Specifications

Form

Lyophilized

Additional notes

Proprietary heat-shock process.

General info

Function

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (Probable). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (PubMed:22677715). The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (Probable). The rank order of affinity is zinc > calcium > magnesium (Probable). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).

Sequence similarities

Belongs to the ALB/AFP/VDB family.

Post-translational modifications

Phosphorylated by FAM20C in the extracellular medium.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

The product is suitable to use in blocking experiments. This product contains:-
Moisture: 1.4%
Ash: 1.4%
Heavy Metals: <0.44ppm
Bovine IgG: Not detected
Protein: 100% (Biuret)
Albumin: 100% (electrophoresis)

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Bovine serum albumin (BSA) also known as albumin bovine serum boasts a molecular weight of approximately 66.5 kDa. This protein is found in the blood serum of cows and is derived from plasma. BSA or bovine albumin acts as a transport protein that binds to various substances including fatty acids and hormones facilitating their movement through the blood. In the lab scientists often use the BSA protein as a standard or blocking agent in experiments particularly in ELISA and Western blot assays.

Biological function summary

Proteins such as BSA play a critical role in maintaining osmotic pressure and pH balance in biological fluids. BSA does not form part of a larger protein complex but interacts with numerous small molecules and ions stabilizing their concentrations in the bloodstream. The interaction of albumin bovine serum with fatty acids is especially important for modulating the bioavailability of lipophilic molecules.

Pathways

Albumin actively contributes to lipid metabolism and transport pathways. It interacts closely with other proteins involved in these processes such as lipoproteins. BSA's binding affinity for various drugs and toxins also places it within the detoxification and excretory pathways ensuring the stability and solubility of these substances in circulation.

Associated diseases and disorders

Scientists have studied BSA for its relevance to liver and kidney diseases given its role in carrying molecules and maintaining osmotic balance. Reduced levels of bovine albumin may indicate liver dysfunction or nephrotic syndrome where protein loss through urine is a concern. BSA's interaction with proteins such as globulin is also significant when investigating these conditions as the balance between these proteins can reflect the body's overall protein status.

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