Native Cow Collagen I protein is a Cow Full Length protein, expressed in Native and suitable for ELISA, WB, IP, SDS-PAGE.
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Key facts
- Expression system
- Native
- Tags
- Tag free
- Applications
- ELISA, WB, IP, SDS-PAGE
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info 1/10000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/1000 | Notes - |
Application IP | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Alternative names
Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
Recommended products
Native Cow Collagen I protein is a Cow Full Length protein, expressed in Native and suitable for ELISA, WB, IP, SDS-PAGE.
Key facts
- Expression system
- Native
- Tags
- Tag free
- Applications
- ELISA, WB, IP, SDS-PAGE
- Biologically active
- No
- Accession
- P02453-1
- Animal free
- No
- Species
- Cow
- Concentration
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 3% Acetic acid
Specifications
- Form
- Liquid
- Additional notes
Bovine Collagen Type 1 has been prepared from Bovine Placenta and is chromatographically and immunologically pure.
General info
- Function
Type I collagen is a member of group I collagen (fibrillar forming collagen).
- Sequence similarities
Belongs to the fibrillar collagen family.
- Post-translational modifications
Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product has been prepared from Bovine Placenta and is chromatographically and immunologically pure. It reacts with anti-Collagen Type I. Reaction with anti-Collagen II, III, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).
This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
- Biological function summary
Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.
- Pathways
Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.
- Associated diseases and disorders
Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.
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1 product image
Image courtesy of an anonymous customer review.ELISA - Native Cow Collagen I protein (ab7526)
ab7526 used to generate a standard curve for ELISA, diluted to a concentration of 4, 2, 1 and 0.5 ug.
Samples were from bovine LD muscle.
Anti-Collagen I + Collagen III antibody ab34710 was used at a 1/1000 dilution as the primary antibody.
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