Native Diphtheria Toxin (mutated G52E) protein

• WB

Lab

Western blot - Native Diphtheria Toxin (mutated G52E) protein (AB188505)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab219834 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-Diphtheria Toxin antibody [IGX3492R-3] (<a href='/en-us/products/primary-antibodies/diphtheria-toxin-antibody-igx3492r-3-ab219834'>ab219834</a>) at 0.5 µg/mL

All lanes:

Western blot - Native Diphtheria Toxin (mutated G52E) protein (ab188505) at 0.1 µg

Secondary

All lanes:

Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 58 kDa

Observed band size: 60 kDa

true

Exposure time: 5s

• WB

Lab

Western blot - Native Diphtheria Toxin (mutated G52E) protein (AB188505)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209329 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-Diphtheria Toxin antibody [IGX3492] (<a href='/en-us/products/primary-antibodies/diphtheria-toxin-antibody-igx3492-ab209329'>ab209329</a>) at 1 µg/mL

All lanes:

Western blot - Native Diphtheria Toxin (mutated G52E) protein (ab188505) at 0.1 µg

Secondary

All lanes:

HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution

Predicted band size: 58 kDa

Observed band size: 60 kDa

true

Exposure time: 5s

• SDS-PAGE

Supplier Data

SDS-PAGE - Native Diphtheria Toxin (mutated G52E) protein (AB188505)

SDS-PAGE analysis of ab188505.