Native Herpes simplex virus HSV2 protein is a Full Length protein and expressed in Native.
Select an associated product type
gE
Herpes Simplex Virus Type 2
Native Herpes simplex virus HSV2 protein is a Full Length protein and expressed in Native.
pH: 7 - 8
Constituents: 99% PBS, 0.1% Triton X-100
Virus particles released from cells are partially purified by low speed centrifugation to remove cell debris. HSV2 particles are purified twice by sucrose density gradients. This process also includes a DNase step to remove cellular DNA and helps to achieve a purity of over 99%. Vortex before use.
HSV2 is propagated in mammalian cells using a protocol optimised for maximum particle yield. At the appropriate time, infected cells are washed in saline then disrupted to release virus particles by freeze thaw and sonication.
Inactivation: 0.1% Triton x-100.
This protein is derived from a pathogenic organism, and may be involved in a disease process, consequently exposure may have adverse health effects.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Herpes Simplex Virus type 2 (HSV2) also known as Human Herpesvirus 2 is a member of the herpesviridae family. It mainly affects the genital region and is a common cause of genital herpes. The virus has a double-stranded DNA genome with a mass of approximately 152 kbp. HSV2 primarily expresses in epithelial cells of the skin and mucous membranes. It can establish latency in neuronal cells especially the sacral ganglia contributing to its ability to persist in the host for life.
HSV2 exhibits a complex life cycle involving both lytic and latent phases. During infection the virus interacts with cellular compartments including the nucleus where it hijacks host cell machinery to replicate its genome. In the latent phase HSV2 becomes dormant in sensory neurons avoiding host immune detection. It is not part of a larger complex but relies on numerous viral proteins for successful replication and invasion such as glycoproteins critical for host entry.
HSV2 interacts with multiple immune evasion and inflammatory pathways. It manipulates the NF-kB signaling pathway an important player in inflammation and immune responses. The virus evades immune detection by inhibiting the activation of NF-kB allowing persistent infection. HSV2 also affects the MAPK signaling pathway through viral proteins such as ICP34.5 which modulate host defense mechanisms and facilitate viral replication.
HSV2 is significantly associated with genital herpes and is linked to an increased risk of HIV acquisition. Genital herpes can cause recurrent painful lesions and can be transmitted to neonates during childbirth leading to neonatal herpes. The virus’s interaction with host immune pathways might also modify the host's susceptibility to other sexually transmitted infections including HIV. HSV2 through its impact on the host’s immune response shares interactions with proteins like HIV-1 gp120 in co-infected individuals which complicates the management of these infections.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com