Native human alpha 1 Antitrypsin protein (Active)
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(2 Publications)
Native human alpha 1 Antitrypsin protein (Active) is a Human Full Length A1AT/SERPIN A1 protein, with >95% purity and suitable for western blot , SDS-PAGE and Functional studies. The predicted molecular weight of ab91136 native protein is 52 kDa.
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View Alternative Names
AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1
- WB
Unknown
Western blot - Native human alpha 1 Antitrypsin protein (Active) (AB91136)
All lanes:
Western blot - Anti-alpha 1 Antitrypsin antibody [B9] (<a href='/en-us/products/primary-antibodies/alpha-1-antitrypsin-antibody-b9-ab9399'>ab9399</a>) at 1 µg/mL
All lanes:
Western blot - Native human alpha 1 Antitrypsin protein (Active) (ab91136) at 0.1 µg
Secondary
All lanes:
Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution
Predicted band size: 46 kDa
true
Exposure time: 30s
- SDS-PAGE
Unknown
SDS-PAGE - Native human alpha 1 Antitrypsin protein (Active) (AB91136)
SDS-PAGE : 4-12% Bis-Tris NuPAGE gel
Lane 1. 5 μg ab91136 (reduced/heated)
Lane 2. 10 μg ab91136 (reduced/heated)
Lane 3. 20 μg ab91136 (reduced/heated)
Lane 4. Molecular weight markers
Lane 5. 5 μg ab91136 (non-reduced/no heat)
Lane 6. 10 μg ab91136 (non-reduced/no heat)
Lane 7. 20 μg ab91136 (non-reduced/no heat)
Reactivity data
Product details
The Native human alpha 1 Antitrypsin protein (Active) ab91136 is prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
Ensure the validity of your result using our native human alpha 1 Antitrypsin (A1AT/SERPIN A1) protein as a control in SDS-PAGE.
Check out our protein gel staining guide for SDS-PAGE here
Protein Determination: Extinction Coefficient (ε) 0.1% at 280 nm, 1 cm pathway = 0.433
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.
Pathways
Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.
Specifications
Form
Lyophilized
General info
Function
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
Sequence similarities
Belongs to the serpin family.
Post-translational modifications
N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.. Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.. (Microbial infection) Proteolytically processed by Staphylococcus aureus seryl, cysteinyl, and metallo-proteases.
Target data
Publications (2)
Recent publications for all applications. Explore the full list and refine your search
Nature genetics 51:1233-1243 PubMed31358993
2019
Applications
Unspecified application
Species
Unspecified reactive species
Journal of neuroinflammation 14:168 PubMed28835277
2017
Applications
Unspecified application
Species
Unspecified reactive species
Complementary Products
Primary Antibodies
AB207303
Anti-alpha 1 Antitrypsin antibody [EPR17087-50]
RabMAb|Recombinant|KO Validated|20ul selling size
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Product promise
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