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Native Human Apolipoprotein B is a Human Full Length protein, expressed in Native, with >95% purity and suitable for SDS-PAGE.

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Images

SDS-PAGE - Native Human Apolipoprotein B (AB91112), expandable thumbnail

Key facts

Purity
>95% SDS-PAGE
Expression system
Native
Tags
Tag free
Applications
SDS-PAGE
Biologically active
No

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Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

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Target data

Function

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

Alternative names

Apolipoprotein B-100, Apo B-100, APOB

Recommended products

Native Human Apolipoprotein B is a Human Full Length protein, expressed in Native, with >95% purity and suitable for SDS-PAGE.

Key facts

Purity
>95% SDS-PAGE
Expression system
Native
Applications
SDS-PAGE
Accession
P04114-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

pH: 10
Constituents: 0.53% Sodium carbonate, 0.415% Sodium deoxycholate, 0.29% Sodium chloride

Sequence info

Amino acid sequence

Accession
P04114
Protein length
Full Length
Nature
Native

Specifications

Form
Lyophilized

General info

Function

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

Post-translational modifications

Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.

Storage

Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Prepared from fresh, non-frozen plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Apolipoprotein B often referred to as ApoB is a core protein component of low-density lipoproteins (LDL) and is essential for their assembly and secretion. ApoB is also known as apolipoproteine B and apolipoprotein B protein. This protein has a large molecular weight approximately 550 kDa. ApoB is primarily expressed in the liver and to some extent in the intestine. Its large size allows it to provide structural integrity to lipoprotein particles playing a role in lipid metabolism.

Biological function summary

ApoB plays a significant role in lipid transport and metabolism. It is a critical component of the lipoprotein particle complex which is responsible for the transport of lipids such as cholesterol and triglycerides through the bloodstream. ApoB helps in the binding of lipoproteins to cell receptors facilitating the uptake of lipids by tissues. Its presence on the surface of lipoproteins makes it important for normal cholesterol homeostasis in the human body.

Pathways

ApoB is integrated within the lipid metabolism pathway. It interacts with proteins such as microsomal triglyceride transfer protein (MTP) during the assembly and secretion of lipoproteins. This pathway is essential for the normal transport of lipids throughout the body and influences processes like absorption of dietary fats. ApoB also plays a role in the pathways related to atherosclerosis where it influences the deposition of cholesterol in arterial walls.

Associated diseases and disorders

ApoB is well-associated with cardiovascular diseases notably atherosclerosis and familial hypercholesterolemia. In these conditions elevated levels of ApoB correlate to an increased risk of plaque formation in blood vessels. ApoB in conjunction with LDL receptor and other related proteins influences lipid homeostasis and disorders where lipid oxidation or accumulation is present. Monitoring ApoB and its concentration can be vital for assessing cardiovascular disease risk.

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1 product image

  • SDS-PAGE - Native Human Apolipoprotein B (ab91112), expandable thumbnail

    SDS-PAGE - Native Human Apolipoprotein B (ab91112)

    SDS-PAGE: 4-12% Bis-Tris-HCI NuPAGE gel

    Lane 1. 5 μg Apolipoprotein (reduced /heated)

    Lane 2. 10 μg Apolipoprotein (reduced/ heated)

    Lane 3. 20 μg Apolipoprotein (reduced/ heated)

    Lane 4. Molecular weight markers

    Lane 5. 5 μg Apolipoprotein (non-reduced/no heat)

    Lane 6. 10 μg Apolipoprotein (non-reduced/no heat)

    Lane 7. 20 μg Apolipoprotein (non-reduced/no heat)

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