Native Human Apolipoprotein CII/ApoC-II is a Human Full Length protein, expressed in Native, with >95% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Native
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Consider this alternative
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of chylomicrons, very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) in plasma. Plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase. Both proapolipoprotein C-II and apolipoprotein C-II can activate lipoprotein lipase. In normolipidemic individuals, it is mainly distributed in the HDL, whereas in hypertriglyceridemic individuals, predominantly found in the VLDL and LDL.
Alternative names
APC2, APOC2, Apolipoprotein C-II, Apo-CII, ApoC-II, Apolipoprotein C2
Recommended products
Native Human Apolipoprotein CII/ApoC-II is a Human Full Length protein, expressed in Native, with >95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Native
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P02655-1
- Animal free
- No
- Species
- Human
- Reconstitution
- After initial centrifugation, we suggest the addition of the lyophilization buffer (10 mM ammonium bicarbonate, pH 7.4) to the original volume, followed by gentle swirling and/or vortexing to ensure adequate homogenization. If further dilution is required, please use the lyophilization buffer.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 0.079% Ammonium bicarbonate
Specifications
- Form
- Lyophilized
General info
- Function
Component of chylomicrons, very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) in plasma. Plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase. Both proapolipoprotein C-II and apolipoprotein C-II can activate lipoprotein lipase. In normolipidemic individuals, it is mainly distributed in the HDL, whereas in hypertriglyceridemic individuals, predominantly found in the VLDL and LDL.
- Sequence similarities
Belongs to the apolipoprotein C2 family.
- Post-translational modifications
Proapolipoprotein C-II is synthesized as a sialic acid containing glycoprotein which is subsequently desialylated prior to its proteolytic processing.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate long-term storage conditions
- -20°C
Notes
Prepared from fresh, non-frozen plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. This product was previously labelled as Apolipoprotein CII
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Apolipoprotein CII (ApoC-II) also known as ApoC2 or ApoC II protein is a small protein with a mass of approximately 8.9 kDa. The protein primarily expresses in the liver and secreted into the bloodstream as a component of very low-density lipoproteins (VLDL) and chylomicrons. In terms of structure ApoC-II comprises part of the apolipoprotein family functioning to mediate lipid metabolism by interacting with lipoproteins in the circulatory system.
- Biological function summary
ApoC-II plays a critical role in lipid metabolism by activating lipoprotein lipase (LPL) an enzyme that hydrolyzes triglycerides into free fatty acids and glycerol. This protein is essential in the chylomicron and VLDL complex that transports triglycerides from the intestines and liver to peripheral tissues. Through this activation of LPL ApoC-II facilitates the breakdown and clearance of triglyceride-rich lipoproteins from the bloodstream maintaining lipid homeostasis.
- Pathways
ApoC-II significantly impacts the lipid metabolism pathway by influencing the catabolism of triglyceride-rich lipoproteins. It therefore plays a role in the lipolytic pathway where it maintains plasma triglyceride levels. Additionally ApoC-II is associated with the LPL pathway with the protein's function closely interacting with other lipoproteins such as ApoC-III which can inhibit LPL activity and modulate the effects of ApoC-II on lipid hydrolysis.
- Associated diseases and disorders
ApoC-II mutations or deficiencies can lead to hyperlipoproteinemia type IB a condition characterized by elevated triglyceride levels due to impaired lipoprotein breakdown. This disorder can result in complications like pancreatitis and cardiovascular disease. Within these disease contexts ApoC-II's interaction with ApoC-III becomes significant as ApoC-III acts as an antagonist to LPL suggesting a balance between these proteins is necessary for maintaining normal lipid metabolism and preventing disorder development.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Native Human Apolipoprotein CII/ApoC-II (ab90925)
SDS-PAGE analysis of ab90925 on an 18% Tris-Glycine Gel
Lane 1: Apolipoprotein CII/ApoC-II - 10 μg (Reduced/heated)
Lane 2: Apolipoprotein CII/ApoC-II - 15 μg (Reduced/heated)
Lane 3: Molecular weight markers
Lane 4: Apolipoprotein CII/ApoC-II - 10 μg (Non-reduced/no heat)
Lane 5: Apolipoprotein CII/ApoC-II - 20 μg (Non-reduced/no heat)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com