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Native Human Collagen I protein is a Human Full Length protein, expressed in Native, with >95% purity and suitable for WB, SDS-PAGE.


Images

Western blot - Native Human Collagen I protein (AB7533), expandable thumbnail

Publications

Key facts

Purity

>95%

Expression system

Native

Tags

Tag free

Applications

WB, SDS-PAGE

Biologically active

No

Reactivity data

Application

WB

Reactivity

Reacts

Dilution info

1/1000

Notes

This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.

Application

SDS-PAGE

Reactivity

Expected

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Type I collagen is a member of group I collagen (fibrillar forming collagen).

Alternative names

Recommended products

Native Human Collagen I protein is a Human Full Length protein, expressed in Native, with >95% purity and suitable for WB, SDS-PAGE.

Key facts

Purity

>95%

Expression system

Native

Applications

WB, SDS-PAGE

Accession
P02452-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 0.003% Acetic acid

Sequence info

Amino acid sequence

Accession

P02452

Protein length

Full Length

Predicted molecular weight

139 kDa

Nature

Native

Specifications

Form

Liquid

Additional notes

Human Collagen type I has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins.

General info

Function

Type I collagen is a member of group I collagen (fibrillar forming collagen).

Sequence similarities

Belongs to the fibrillar collagen family.

Post-translational modifications

Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type I. Reaction with anti-Collagen II, III, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.

Biological function summary

Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.

Pathways

Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.

Associated diseases and disorders

Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.

Product promise

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1 product image

  • Western blot - Native Human Collagen I protein (ab7533), expandable thumbnail

    Western blot - Native Human Collagen I protein (ab7533)

    DyLight 649 anti-rabbit secondary antibody at 1:20,000 for 30 min at RT.

    Blocking Buffer for 30 min at room temperature - proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.

    Other Band(s): Collagen Type I splice variants and isoforms.

    All lanes: Western blot - Anti-Collagen I + Collagen III antibody (Anti-Collagen I + Collagen III antibody ab34710) at 1/1000 dilution

    All lanes: Western blot - Native Human Collagen I protein (ab7533) at 0.05 µg

    Secondary

    All lanes: DyLight™ 649 anti-rabbit secondary antibody at 1/20000 dilution

    Predicted band size: 139 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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