Native Human Collagen I protein is a Human Full Length protein, expressed in Native, with >95% purity and suitable for WB, SDS-PAGE.
>95%
Native
Tag free
WB, SDS-PAGE
No
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application WB | Reactivity Reacts | Dilution info 1/1000 | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Application SDS-PAGE | Reactivity Expected | Dilution info - | Notes - |
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Type I collagen is a member of group I collagen (fibrillar forming collagen).
Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
Native Human Collagen I protein is a Human Full Length protein, expressed in Native, with >95% purity and suitable for WB, SDS-PAGE.
>95%
Native
Tag free
WB, SDS-PAGE
No
No
Human
Preservative: 0.01% Sodium azide
Constituents: 0.003% Acetic acid
Full Length
139 kDa
Native
Liquid
Human Collagen type I has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins.
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Belongs to the fibrillar collagen family.
Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type I. Reaction with anti-Collagen II, III, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).
This supplementary information is collated from multiple sources and compiled automatically.
Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.
Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.
Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.
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Terms & Conditions.
DyLight™ 649 anti-rabbit secondary antibody at 1:20,000 for 30 min at RT.
Blocking Buffer for 30 min at room temperature - proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.
Other Band(s): Collagen Type I splice variants and isoforms.
All lanes: Western blot - Anti-Collagen I + Collagen III antibody (Anti-Collagen I + Collagen III antibody ab34710) at 1/1000 dilution
All lanes: Western blot - Native Human Collagen I protein (ab7533) at 0.05 µg
All lanes: DyLight™ 649 anti-rabbit secondary antibody at 1/20000 dilution
Predicted band size: 139 kDa
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