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Native Human Collagen III protein is a Human Full Length protein, expressed in Native and suitable for ELISA, IP, SDS-PAGE, WB.

Expression system

Native

Tags

Tag free

Applications

ELISA, IP, SDS-PAGE, WB

Biologically active

No

Reactivity data

Application
ELISA
Reactivity
Reacts
Dilution info
1/10000
Notes

-

Application
IP
Reactivity
Reacts
Dilution info
1/100 - 1/500
Notes

This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
1/1000
Notes

-

Images

Target data

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Native Human Collagen III protein is a Human Full Length protein, expressed in Native and suitable for ELISA, IP, SDS-PAGE, WB.

Key facts

Expression system

Native

Applications

ELISA, IP, SDS-PAGE, WB

Accession
P02461-1
Protein length

Full Length

Animal free

No

Nature

Native

Species

Human

Concentration
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Storage buffer

Constituents: 3% Acetic acid

Specifications

Form

Liquid

Additional notes

Human Collagen Type III has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins.

General info

Function

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.

Sequence similarities

Belongs to the fibrillar collagen family.

Post-translational modifications

Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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