Native human Factor Xa is a Human Full Length protein with >95% purity and suitable for SDS-PAGE and Functional studies. The predicted molecular weight of ab62229 native protein is 46 kDa.
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View Alternative Names
Coagulation factor X, Stuart factor, Stuart-Prower factor, F10
- SDS-PAGE
Supplier Data
SDS-PAGE - Native human Factor Xa (AB62229)
SDS-PAGE analysis of ab62229 for 1ug protein.
Novex 4-12% Bis-Tris Gel
NR= non-reduced; R = reduced
Reactivity data
Product details
The Native Human Factor Xa ab62229 is prepared from plasma shown to be non reactive for anti-HIV-1/2, HIV-1 antigen(s), HBsAg, STS, anti-HCV, anti-HBcore and anti-HTLV I & II.
The bioactivity of Native Human Factor Xa ab62229 was determined by Factor X clotting assay. One (1) unit is equivalent to the Factor X activity in one (1) milliliter of normal human plasma.
Check out our protein gel staining guide for SDS-PAGE here
Protein Determination: Extinction Coefficient (ε) 1% at 280 nm, 1cm pathway = 11.6
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Activated factor X plays a pivotal role in converting prothrombin into thrombin facilitating the cross-linking of fibrin which stabilizes the forming clot. Factor Xa while acting as a monomer often associates with factor Va to form prothrombinase complex enhancing its efficiency. The prothrombinase complex accelerates thrombin generation exponentially which is an essential step in hemostasis.
Pathways
The blood coagulation cascade involves factor Xa prominently within the intrinsic and extrinsic pathways. Factor Xa lies downstream in these pathways and links them to the common pathway. It directly interacts with thrombin and factor Va forming an integral part of both pathways in the conversion of prothrombin to thrombin. These steps are important in maintaining hemostasis and enabling proper clot formation during vascular injury.
Specifications
Form
Liquid
General info
Function
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting (PubMed : 22409427). Factor Xa activates pro-inflammatory signaling pathways in a protease-activated receptor (PAR)-dependent manner (PubMed : 24041930, PubMed : 30568593, PubMed : 34831181, PubMed : 18202198). Up-regulates expression of protease-activated receptors (PARs) F2R, F2RL1 and F2RL2 in dermal microvascular endothelial cells (PubMed : 35738824). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, in cardiac fibroblasts and umbilical vein endothelial cells in PAR-1/F2R-dependent manner (PubMed : 30568593, PubMed : 34831181). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2, IL6, TNF-alpha/TNF, IL-1beta/IL1B, IL8/CXCL8 and IL18, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Induces expression of adhesion molecules, such as ICAM1, VCAM1 and SELE, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Increases expression of phosphorylated ERK1/2 in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Triggers activation of the transcription factor NF-kappa-B in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Activates pro-inflammatory and pro-fibrotic responses in dermal fibroblasts and enhances wound healing probably via PAR-2/F2RL1-dependent mechanism (PubMed : 18202198). Activates barrier protective signaling responses in endothelial cells in PAR-2/F2RL1-dependent manner; the activity depends on the cleavage of PAR-2/F2RL1 by factor Xa (PubMed : 22409427). Up-regulates expression of plasminogen activator inhibitor 1 (SERPINE1) in atrial tissues (PubMed : 24041930).
Sequence similarities
Belongs to the peptidase S1 family.
Post-translational modifications
The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.. Proteolytically cleaved and activated by cathepsin CTSG (PubMed:8920993). The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway) (By similarity).. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Target data
Publications (2)
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iScience 25:104547 PubMed35754738
2022
Applications
Unspecified application
Species
Unspecified reactive species
Acta biomaterialia 137:331-345 PubMed34673227
2021
Applications
Unspecified application
Species
Unspecified reactive species
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