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Native Human IgG protein is a Human Full Length protein with >95% purity and suitable for SDS-PAGE.. The predicted molecular weight of ab91102 native protein is 150 kDa

- Save time and ensure accurate results - use our IgG protein as an isotype control
- No cross-reaction from other immunoglobulin isotypes

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Images

SDS-PAGE - Native Human IgG protein (AB91102), expandable thumbnail

Publications

Key facts

Purity
>95% SDS-PAGE
Expression system
Native
Tags
Tag free
Applications
SDS-PAGE
Biologically active
No

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.

Additional Targets

, ,

Alternative names

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1

Recommended products

Native Human IgG protein is a Human Full Length protein with >95% purity and suitable for SDS-PAGE.. The predicted molecular weight of ab91102 native protein is 150 kDa

- Save time and ensure accurate results - use our IgG protein as an isotype control
- No cross-reaction from other immunoglobulin isotypes

Key facts

Purity
>95% SDS-PAGE
Expression system
Native
Applications
SDS-PAGE
Accession
P01857-1
Animal free
No
Species
Human
Reconstitution
Reconstitute in water
Concentration
Loading...
Storage buffer

pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: Phosphate Buffer, 0.87% Sodium chloride

Sequence info

Amino acid sequence

Accession
P01857
Protein length
Full Length
Predicted molecular weight
150 kDa
Nature
Native

Specifications

Form
Lyophilized

General info

Function

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.

Post-translational modifications

Glycosylation on Asn-180 is required for interaction with Fc receptors and ability to activate the complement pathway.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes

Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.36.

Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.

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1 product image

  • SDS-PAGE - Native Human IgG protein (ab91102), expandable thumbnail

    SDS-PAGE - Native Human IgG protein (ab91102)

    SDS-PAGE: 4-12% Bis-Tris NuPAGE gel

    Lane 1. Molecular weight markers

    Lane 2. 5 μg IgG (reduced/heated)

    Lane 3. 10 μg IgG (reduced/heated)

    Lane 4. 20 μg IgG (reduced/heated)

    Lane 5. 5 μg IgG (non-reduced/no heat)

    Lane 6. 10 μg IgG non-reduced/no heat)

    Lane 7. 20 μg IgG non-reduced/no heat)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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