Native Human IgG4 protein is a Human Full Length Immunoglobulin G4 (IgG4) protein with >95% purity and suitable for SDS-PAGE. The predicted molecular weight of ab183266 native protein is 146 kDa.
-Save time and ensure accurate results - use ab183266 as an IgG4 isotype control
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
The protein expressed by the IGHG4 gene is part of the constant region of immunoglobulin heavy chains. Immunoglobulins, or antibodies, are glycoproteins produced by B lymphocytes that can be membrane-bound or secreted. During humoral immunity's recognition phase, membrane-bound immunoglobulins act as receptors. When they bind a specific antigen, they trigger B lymphocytes to undergo clonal expansion and differentiate into plasma cells that secrete immunoglobulins. These secreted immunoglobulins then mediate the effector phase of humoral immunity to eliminate the bound antigens. Each immunoglobulin possesses two antigen binding sites, formed by the variable domains of a heavy chain and an associated light chain, allowing for high affinity binding to specific antigens. These variable domains undergo V-(D)-J rearrangement and may experience somatic hypermutations, facilitating affinity maturation after antigen exposure and selection. This supplementary information is collated from multiple sources and compiled automatically.
Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4
Native Human IgG4 protein is a Human Full Length Immunoglobulin G4 (IgG4) protein with >95% purity and suitable for SDS-PAGE. The predicted molecular weight of ab183266 native protein is 146 kDa.
-Save time and ensure accurate results - use ab183266 as an IgG4 isotype control
pH: 7.4
Constituents: 0.88% Sodium chloride, 0.33% Sodium phosphate
The protein expressed by the IGHG4 gene is part of the constant region of immunoglobulin heavy chains. Immunoglobulins, or antibodies, are glycoproteins produced by B lymphocytes that can be membrane-bound or secreted. During humoral immunity's recognition phase, membrane-bound immunoglobulins act as receptors. When they bind a specific antigen, they trigger B lymphocytes to undergo clonal expansion and differentiate into plasma cells that secrete immunoglobulins. These secreted immunoglobulins then mediate the effector phase of humoral immunity to eliminate the bound antigens. Each immunoglobulin possesses two antigen binding sites, formed by the variable domains of a heavy chain and an associated light chain, allowing for high affinity binding to specific antigens. These variable domains undergo V-(D)-J rearrangement and may experience somatic hypermutations, facilitating affinity maturation after antigen exposure and selection.
This supplementary information is collated from multiple sources and compiled automatically.
Glycosylation on Asn-177 is required for interaction with Fc receptors and ability to activate the complement pathway.
Ensure the validity of your result using our IgG4 protein ab183266 as an isotype control. ab183266 can be used as a positive control in SDS-PAGE.
Check out our Electrophoresis guide for SDS-PAGE here.
ab183266 was prepared from normal plasma, shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
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