Native human LDL protein is a Full Length protein with >95% purity and suitable for Functional studies and SDS-PAGE. The predicted molecular weight of ab91115 native protein is 2300 kDa.
- Save time and ensure accurate results - use our Low density lipoprotein (LDL) as a control
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- Pharmaceutics 14:2021Effects of Adipose-Derived Biogenic Nanoparticle-Associated microRNA-451a on Toll-like Receptor 4-Induced Cytokines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinghua Wang,Anthony Pham,Lu Kang,Sierra A Walker,Irina Davidovich,Dalila Iannotta,Sarvam P TerKonda,Shane Shapiro,Yeshayahu Talmon,Si Pham,Joy WolframPubMed 35056912
- Translational lung cancer research 10:128-1422021Cholesterol synthesis disruption combined with a molecule-targeted drug is a promising metabolic therapy for EGFR mutant non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYouli Luo,Yunpeng Yang,Peijian Peng,Jianhua Zhan,Zhihui Wang,Zhiquan Zhu,Zhonghan Zhang,Lin Liu,Wenfeng Fang,Li ZhangPubMed 33569299
- Frontiers in neurology 10:322019Long Noncoding RNA-H19 Contributes to Atherosclerosis and Induces Ischemic Stroke via the Upregulation of Acid Phosphatase 5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYujing Huang et. al.PubMed 30778327
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Native
- Tags
- Tag free
- Applications
- FuncS, SDS-PAGE
- Biologically active
- Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
Low density lipoprotein
Recommended products
Native human LDL protein is a Full Length protein with >95% purity and suitable for Functional studies and SDS-PAGE. The predicted molecular weight of ab91115 native protein is 2300 kDa.
- Save time and ensure accurate results - use our Low density lipoprotein (LDL) as a control
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Native
- Tags
- Tag free
- Applications
- FuncS, SDS-PAGE
- Biologically active
- Yes
- Biological activity
- Active
- Animal free
- No
- Concentration
- Storage buffer
pH: 7.4
Constituents: 0.87% Sodium chloride, 0.01% EDTA
Sequence info
Amino acid sequence
- Protein length
- Full Length
- Actual molecular weight
- 2300 kDa
- Nature
- Native
Specifications
- Form
- Liquid
- Additional notes
Purity: single arc by IEP against antisera to whole human serum. Essentially free of other plasma lipoproteins as determined by electrophoresis using Fat Red 7B stain for lipids and Coomassie Blue for proteins. >=95% of total lipoprotein content by electrophoresis.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze, Store in the dark
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
The Native Human LDL (lipoprotein-Low density) protein ab91115 is prepared from fresh, non-frozen p plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
Ensure the validity of your result using our native human LDL protein as a positive control in SDS-PAGE.
Essentially free of other plasma lipoproteins and chylomicrons as determined by electrophoresis using a SPIFE Vis Cholesterol gel kit for lipids and Coomassie Blue for proteins.
LDL Protein Properties
Density: 1.019 to 1.063 g/ml
Composition: 78-80% lipid, 22-20% protein
Check out our protein gel staining guide for SDS-PAGE here
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Low-density lipoprotein often referred to as LDL or "bad cholesterol" serves as a carrier for lipids in the bloodstream. LDL particles are spherical and contain an apolipoprotein B-100 shell with a core mostly filled with cholesterol esters. It has an approximate molecular weight of 2536 kDa. LDL is mainly expressed in the liver where it is released into the circulation to deliver cholesterol to various tissues. It is an essential component that transports cholesterol and other lipids through the body for structural and metabolic functions.
- Biological function summary
LDL plays an important role by supplying cholesterol to cells which is critical for membrane structure and hormone synthesis. LDL binds to specific receptors on the cell surface prompting its cellular uptake through the LDL uptake assay mechanisms including receptor-mediated endocytosis. This process involves the LDL protein engaging with a receptor complex facilitating the internalization of cholesterol for cell usage or storage. Native LDL can be distinguished from oxidized forms which have different roles in health and disease.
- Pathways
LDL is vital in cholesterol homeostasis and lipid metabolism pathways. One notable pathway includes its interaction in the mevalonate pathway essential for cholesterol biosynthesis. The interaction with the insulin signaling pathway also highlights the significance of LDL in energy homeostasis. Related proteins such as high-density lipoprotein (HDL) and very low-density lipoprotein (VLDL) work in tandem with LDL forming a complex system for managing lipid transport and affecting cholesterol levels.
- Associated diseases and disorders
LDL is closely associated with atherosclerosis and cardiovascular disease. High LDL levels lead to cholesterol accumulation in arterial walls promoting the formation of plaques and increasing the risk of heart disease. Apolipoprotein B-100 part of the LDL structure is often investigated in the context of these diseases because it mediates LDL's attachment to arterial walls. Understanding LDL's relationship with these disorders highlights the importance of controlling its levels through medical and lifestyle interventions therefore minimizing disease risk.
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1 product image
SDS-PAGE - Native human LDL protein (ab91115)
SDS-PAGE: 12% Tris HCl gel
10 μg LDL (reduced / heat)
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