Native human PF4 protein is a Human Full Length Platelet Factor 4 protein, in the 32 to 101 aa range with >95% purity and suitable for SDS-PAGE. The predicted molecular weight of ab81754 native protein is 7.8 kDa.
- Save time and ensure accurate results - use our PF4(CXCL4) protein
- Heparin-neutralizing activity verified by clotting assay
- Marker of platelet activation
- Optimal protein bioactivity and stability
View Alternative Names
CXCL4, SCYB4, PF4, Platelet factor 4, PF-4, C-X-C motif chemokine 4, Iroplact, Oncostatin-A
- SDS-PAGE
Supplier Data
SDS-PAGE - Native human PF4 protein (AB81754)
SDS-PAGE analysis of ab81754 for 1ug protein.
Novex 4-12% Bis-Tris Gel
NR= non-reduced; R = reduced
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Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR7763] - BSA and Azide free (AB248336)](https://content.abcam.com/products/images/pf4-antibody-epr7763-bsa-and-azide-free-ab248336--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118764.jpg)
Reactivity data
Product details
Ensure the validity of your result using our native human Platelet Factor 4 (PF4/CXCL4) protein as a control in SDS-PAGE.
Check out our protein gel staining guide for SDS-PAGE here
In its native state, PF4 is a homotetramer which exists in complex with a high molecular weight proteoglycan carrier protein. The heparin-binding domain is presumably localized within a COOH-terminal region of the molecule where several lysine residues are clustered.
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PF4 plays an important role in modulating the immune response and coagulation processes. PF4 is not part of a stable protein complex but can interact dynamically with heparin on the surface of endothelial cells and glycosaminoglycans. It inhibits the action of heparin promoting clot formation and angiogenesis. PF4 also attracts and activates different types of leukocytes and can regulate inflammatory processes influencing how the immune system responds to injury.
Pathways
PF4 significantly interacts with both the coagulation and inflammation pathways. In the coagulation pathway PF4 acts to neutralize heparin-like molecules thereby enhancing thrombosis. It works closely with proteins such as thrombin and fibrinogen contributing to the blood clot cascade. Within the inflammatory pathway PF4's chemokine activity helps direct leukocytes to sites of tissue damage or infection linking it to cytokines and other chemokines that mediate immune responses.
Specifications
Form
Liquid
General info
Function
Chemokine released during platelet aggregation that plays a role in different biological processes including hematopoiesis, cell proliferation, differentiation, and activation (PubMed : 29930254, PubMed : 9531587). Acts via different functional receptors including CCR1, CXCR3A or CXCR3B (PubMed : 18174362, PubMed : 29930254). Upon interaction with CXCR3A receptor, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling (PubMed : 18174362, PubMed : 24469069). Neutralizes the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Plays a role in the inhibition of hematopoiesis and in the maintenance of hematopoietic stem cell (HSC) quiescence (PubMed : 9531587). Chemotactic for neutrophils and monocytes via CCR1 (PubMed : 29930254). Inhibits endothelial cell proliferation. In cooperation with toll-like receptor 8/TLR8, induces chromatin remodeling and activates inflammatory gene expression via the TBK1-IRF5 axis (PubMed : 35701499). In addition, induces myofibroblast differentiation and collagen synthesis in different precursor cells, including endothelial cells, by stimulating endothelial-to-mesenchymal transition (PubMed : 34986347). Interacts with thrombomodulin/THBD to enhance the activation of protein C and thus potentiates its anticoagulant activity (PubMed : 9395524).
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family.
Target data
Product promise
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