Native Integrin alpha 2b + beta 3 protein (Activation site) is a Human Full Length protein, in the 1 to 1039 aa range, expressed in Native, with >95% purity and suitable for SDS-PAGE, FuncS.
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Publications
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- Bio-protocol 9:e31562019Measurement of redox states of the β3 integrin disulfide bonds by Mass Spectrometry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoyce ChiuPubMed 33654965
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Native
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Sequence
This product is comprised of multiple sequences see
Amino acid sequence 1
M A R A L C P L Q A L W L L E W V L L L L G P C A A P P A W A L N L D P V Q L T F Y A G P N G S Q F G F S L D F H K D S H G R V A I V V G A P R T L G P S Q E E T G G V F L C P W R A E G G Q C P S L L F D L R D E T R N V G S Q T L Q T F K A R Q G L G A S V V S W S D V I V A C A P W Q H W N V L E K T E E A E K T P V G S C F L A Q P E S G R R A E Y S P C R G N T L S R I Y V E N D F S W D K R Y C E A G F S S V V T Q A G E L V L G A P G G Y Y F L G L L A Q A P V A D I F S S Y R P G I L L W H V S S Q S L S F D S S N P E Y F D G Y W G Y S V A V G E F D G D L N T T E Y V V G A P T W S W T L G A V E I L D S Y Y Q R L H R L R G E Q M A S Y F G H S V A V T D V N G D G R H D L L V G A P L Y M E S R A D R K L A E V G R V Y L F L Q P R G P H A L G A P S L L L T G T Q L Y G R F G S A I A P L G D L D R D G Y N D I A V A A P Y G G P S G R G Q V L V F L G Q S E G L R S R P S Q V L D S P F P T G S A F G F S L R G A V D I D D N G Y P D L I V G A Y G A N Q V A V Y R A Q P V V K A S V Q L L V Q D S L N P A V K S C V L P Q T K T P V S C F N I Q M C V G A T G H N I P Q K L S L N A E L Q L D R Q K P R Q G R R V L L L G S Q Q A G T T L N L D L G G K H S P I C H T T M A F L R D E A D F R D K L S P I V L S L N V S L P P T E A G M A P A V V L H G D T H V Q E Q T R I V L D C G E D D V C V P Q L Q L T A S V T G S P L L V G A D N V L E L Q M D A A N E G E G A Y E A E L A V H L P Q G A H Y M R A L S N V E G F E R L I C N Q K K E N E T R V V L C E L G N P M K K N A Q I G I A M L V S V G N L E E A G E S V S F Q L Q I R S K N S Q N P N S K I V L L D V P V R A E A Q V E L R G N S F P A S L V V A A E E G E R E Q N S L D S W G P K V E H T Y E L H N N G P G T V N G L H L S I H L P G Q S Q P S D L L Y I L D I Q P Q G G L Q C F P Q P P V N P L K V D W G L P I P S P S P I H P A H H K R D R R Q I F L P E P E Q P S R L Q D P V L V S C D S A P C T V V Q C D L Q E M A R G Q R A M V T V L A F L W L P S L Y Q R P L D Q F V L Q S H A W F N V S S L P Y A V P P L S L P R G E A Q V W T Q L L R A L E E R A I P I W W V L V G V L G G L L L L T I L V L A M W K V G F F K R N R P P L E E D D E E G E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain (By similarity). Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen (PubMed:9111081). This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface (By similarity).
Additional Targets
ITGB3
Alternative names
CD41, GP2B, ITGAB, ITGA2B, Integrin alpha-IIb, GPalpha IIb, Platelet membrane glycoprotein IIb, GPIIb
Recommended products
Native Integrin alpha 2b + beta 3 protein (Activation site) is a Human Full Length protein, in the 1 to 1039 aa range, expressed in Native, with >95% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Native
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Active
- Accession
- P08514-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.1% Triton X-100, 0.0111% Calcium chloride
Sequence info
Amino acid sequence 1
- M A R A L C P L Q A L W L L E W V L L L L G P C A A P P A W A L N L D P V Q L T F Y A G P N G S Q F G F S L D F H K D S H G R V A I V V G A P R T L G P S Q E E T G G V F L C P W R A E G G Q C P S L L F D L R D E T R N V G S Q T L Q T F K A R Q G L G A S V V S W S D V I V A C A P W Q H W N V L E K T E E A E K T P V G S C F L A Q P E S G R R A E Y S P C R G N T L S R I Y V E N D F S W D K R Y C E A G F S S V V T Q A G E L V L G A P G G Y Y F L G L L A Q A P V A D I F S S Y R P G I L L W H V S S Q S L S F D S S N P E Y F D G Y W G Y S V A V G E F D G D L N T T E Y V V G A P T W S W T L G A V E I L D S Y Y Q R L H R L R G E Q M A S Y F G H S V A V T D V N G D G R H D L L V G A P L Y M E S R A D R K L A E V G R V Y L F L Q P R G P H A L G A P S L L L T G T Q L Y G R F G S A I A P L G D L D R D G Y N D I A V A A P Y G G P S G R G Q V L V F L G Q S E G L R S R P S Q V L D S P F P T G S A F G F S L R G A V D I D D N G Y P D L I V G A Y G A N Q V A V Y R A Q P V V K A S V Q L L V Q D S L N P A V K S C V L P Q T K T P V S C F N I Q M C V G A T G H N I P Q K L S L N A E L Q L D R Q K P R Q G R R V L L L G S Q Q A G T T L N L D L G G K H S P I C H T T M A F L R D E A D F R D K L S P I V L S L N V S L P P T E A G M A P A V V L H G D T H V Q E Q T R I V L D C G E D D V C V P Q L Q L T A S V T G S P L L V G A D N V L E L Q M D A A N E G E G A Y E A E L A V H L P Q G A H Y M R A L S N V E G F E R L I C N Q K K E N E T R V V L C E L G N P M K K N A Q I G I A M L V S V G N L E E A G E S V S F Q L Q I R S K N S Q N P N S K I V L L D V P V R A E A Q V E L R G N S F P A S L V V A A E E G E R E Q N S L D S W G P K V E H T Y E L H N N G P G T V N G L H L S I H L P G Q S Q P S D L L Y I L D I Q P Q G G L Q C F P Q P P V N P L K V D W G L P I P S P S P I H P A H H K R D R R Q I F L P E P E Q P S R L Q D P V L V S C D S A P C T V V Q C D L Q E M A R G Q R A M V T V L A F L W L P S L Y Q R P L D Q F V L Q S H A W F N V S S L P Y A V P P L S L P R G E A Q V W T Q L L R A L E E R A I P I W W V L V G V L G G L L L L T I L V L A M W K V G F F K R N R P P L E E D D E E G E
- Accession
- P08514
- Protein length
- Full Length
- Amino acids
- 1 to 1039
- Nature
- Native
Amino acid sequence 2
- M R A R P R P R P L W A T V L A L G A L A G V G V G G P N I C T T R G V S S C Q Q C L A V S P M C A W C S D E A L P L G S P R C D L K E N L L K D N C A P E S I E F P V S E A R V L E D R P L S D K G S G D S S Q V T Q V S P Q R I A L R L R P D D S K N F S I Q V R Q V E D Y P V D I Y Y L M D L S Y S M K D D L W S I Q N L G T K L A T Q M R K L T S N L R I G F G A F V D K P V S P Y M Y I S P P E A L E N P C Y D M K T T C L P M F G Y K H V L T L T D Q V T R F N E E V K K Q S V S R N R D A P E G G F D A I M Q A T V C D E K I G W R N D A S H L L V F T T D A K T H I A L D G R L A G I V Q P N D G Q C H V G S D N H Y S A S T T M D Y P S L G L M T E K L S Q K N I N L I F A V T E N V V N L Y Q N Y S E L I P G T T V G V L S M D S S N V L Q L I V D A Y G K I R S K V E L E V R D L P E E L S L S F N A T C L N N E V I P G L K S C M G L K I G D T V S F S I E A K V R G C P Q E K E K S F T I K P V G F K D S L I V Q V T F D C D C A C Q A Q A E P N S H R C N N G N G T F E C G V C R C G P G W L G S Q C E C S E E D Y R P S Q Q D E C S P R E G Q P V C S Q R G E C L C G Q C V C H S S D F G K I T G K Y C E C D D F S C V R Y K G E M C S G H G Q C S C G D C L C D S D W T G Y Y C N C T T R T D T C M S S N G L L C S G R G K C E C G S C V C I Q P G S Y G D T C E K C P T C P D A C T F K K E C V E C K K F D R G A L H D E N T C N R Y C R D E I E S V K E L K D T G K D A V N C T Y K N E D D C V V R F Q Y Y E D S S G K S I L Y V V E E P E C P K G P D I L V V L L S V M G A I L L I G L A A L L I W K L L I T I H D R K E F A K F E E E R A R A K W D T A N N P L Y K E A T S T F T N I T Y R G T
- Accession
- P05106
- Protein length
- Full Length
- Amino acids
- 1 to 788
- Nature
- Native
Specifications
- Form
- Liquid
General info
- Function
Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain (By similarity). Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen (PubMed:9111081). This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface (By similarity).
- Sequence similarities
Belongs to the integrin alpha chain family.
- Post-translational modifications
Integrin alpha-IIb heavy chain
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Extinction coefficient: 0.91
Protein concentration determined via the Bradford method.
Host species: Human.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Integrin alpha 2b + beta 3 also known as glycoprotein IIb/IIIa is an important heterodimeric integrin protein complex essential for platelet adhesion and aggregation. This integrin with a mass around 210 kDa functions as a receptor for fibrinogen and other adhesive proteins. Expression of integrin alpha 2b beta 3 is found mainly in platelets and megakaryocytes. The integrin protein's alpha 2b subunit contributes to its specificity and is involved in binding to the ligand while the beta 3 subunit influences activation and signal transduction.
- Biological function summary
Integrin alpha 2b beta 3 plays a major role in hemostasis by mediating platelet cohesion and clot formation at sites of vascular injury. This integrin is an important part of the platelet glycoprotein complex that forms a bridge to link platelets through fibrinogen. Through its function integrin alpha 2b beta 3 is responsible for ensuring the stability of clots in blood vessels preventing loss of blood. It also interacts with various signaling molecules inside platelets facilitating processes like shape change and granule secretion.
- Pathways
Integrin alpha 2b beta 3 involvement in hemostatic pathways reveals its importance in thrombocytopenic mechanisms. Inside the platelet signaling pathways such as phosphatidylinositol 3-kinase (PI3K) and Src family kinases become activated upon ligand binding to this integrin. These pathways involve other proteins like talin and kindlin which play roles in integrin inside-out activation. Additionally crosstalk with other integrins reinforces platelet adhesion and spreading.
- Associated diseases and disorders
Integrin alpha 2b beta 3 associations with thrombotic disorders highlight its clinical significance. Its dysregulation can lead to conditions such as myocardial infarction and stroke where excessive platelet aggregation causes vascular blockages. The integrin also connects to disorders like Glanzmann's thrombasthenia a condition resulting from mutations in the integrin alpha 2b or beta 3 subunits leading to impaired clot formation. The integrin protein's interaction with fibrinogen and von Willebrand factor further emphasizes its impact on thrombotic diseases.
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1 product image
SDS-PAGE - Native Integrin alpha 2b + beta 3 protein (Activation site) (ab95130)
6 ug of ab95130 on SDS-PAGE.
Lane 1: Non-reduced.
Lane 2: Reduced.
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