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AB96742

Native mouse Factor X protein

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Native mouse Factor X protein is a Mouse Full Length protein, in the 41 to 481 aa range, expressed in Native, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Coagulation factor X, Stuart factor, F10

Key facts

Purity

>95% SDS-PAGE

Expression system

Native

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

86.8 U/mg determined by Factor X clotting assay (1 unit is equivalent to the Factor X activity in 1 ml normal plasma based on a Human plasma standard curve). 86.8 U/mg determined by Factor X clotting assay (1 unit is equivalent to the Factor X activity in 1 ml normal plasma based on a Human plasma standard curve).

Accession

O88947

Animal free

No

Carrier free

No

Species

Mouse

Storage buffer

Constituents: 50% Glycerol (glycerin, glycerine)

storage-buffer

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB9485

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Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

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Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

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Assay Kits

AB289650

Human Factor X ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Factor X ELISA Kit (AB289650)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"58.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":481,"aminoAcidStart":41,"nature":"Native","expressionSystem":null,"accessionNumber":"O88947","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor X also known as Stuart-Prower Factor is a critical component in the coagulation cascade. It has a molecular weight of approximately 59 kDa and is expressed mainly in the liver. This serine protease plays an important role in the conversion of prothrombin to thrombin an essential step in blood clot formation. Factor X circulates in the plasma as an inactive zymogen and becomes activated to its enzyme form factor Xa in response to specific physiological signals.
Biological function summary

Factor X contributes significantly to the coagulation process by activating prothrombin into thrombin. It is not part of a larger complex but functions closely with other components of the coagulation cascade to maintain hemostasis. Factor X interacts with calcium ions and phospholipids on the surface of platelets enhancing its enzymatic activity. This activation ensures proper regulation of blood clotting preventing excessive bleeding or uncontrolled clot formation.

Pathways

Factor X is central to both the intrinsic and extrinsic coagulation pathways. It interacts with proteins such as factor VIIa in the extrinsic pathway and factor IXa in the intrinsic pathway both pathways leading to the activation of factor Xa. In turn factor Xa is key in the common pathway which combines inputs from the intrinsic and extrinsic pathways to produce thrombin essential for fibrin clot formation.

Factor X is associated with bleeding disorders such as hemophilia and liver disease. Deficiencies or dysfunctions in factor X result in inadequate clot formation leading to prolonged bleeding episodes. Furthermore increased factor X activity as in cases of thrombophilia can raise the risk of abnormal blood clots. Factor X works alongside proteins like factor V with both deficiencies playing roles in hemophilia. Monitoring factor X activity is important in diagnosing and managing these coagulation-related conditions.
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Specifications

Form

Liquid

Additional notes

ab96742 is isolated from fresh frozen plasma by a combination of conventional techniques and immunoaffinity chromatography.

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General info

Function

Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting (By similarity). Factor Xa activates pro-inflammatory and pro-fibrotic signaling pathways in a protease-activated receptor (PAR)-dependent manner (By similarity).

Sequence similarities

Belongs to the peptidase S1 family.

Post-translational modifications

Carboxylated by vitamin K-dependent GGCX to form gamma-carboxyglutamate; these residues are essential for the binding of calcium.. N- and O-glycosylated.. Proteolytically cleaved and activated by cathepsin CTSG (By similarity). The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway) (By similarity).. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

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Product protocols

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Target data

Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting (By similarity). Factor Xa activates pro-inflammatory and pro-fibrotic signaling pathways in a protease-activated receptor (PAR)-dependent manner (By similarity).
See full target information F10
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