Native Staphylococcus aureus Protein A (Alkaline Phosphatase) is a Staphylococcus aureus Full Length protein, expressed in Native, with >95% purity and suitable for ELISA, WB, Dot, IHC-Fr, IHC-P.
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
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Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (By similarity). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).
Protein A
Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SpA, spa
Native Staphylococcus aureus Protein A (Alkaline Phosphatase) is a Staphylococcus aureus Full Length protein, expressed in Native, with >95% purity and suitable for ELISA, WB, Dot, IHC-Fr, IHC-P.
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1.5% BSA, 0.87% Sodium chloride, 0.79% Tris HCl, 0.0095% Magnesium chloride, 0.00136% Zinc chloride
Protein A Alkaline Phosphatase Conjugated was prepared from chromatographically pure Protein A.
Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (By similarity). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).
Belongs to the immunoglobulin-binding protein SpA family.
Protein A Concentration: 1.0 mg/ml (by UV absorbance at 280 nm) This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the stock concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.
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