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AB7345

Native Staphylococcus aureus Protein A (Alkaline Phosphatase)

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Native Staphylococcus aureus Protein A (Alkaline Phosphatase) is a Staphylococcus aureus subsp. aureus NCTC 8325 Full Length protein, expressed in Native, with >95%, suitable for ELISA, WB, Dot, IHC-Fr, IHC-P.

View Alternative Names

SAOUHSC_00069, spa, Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SpA

Key facts

Purity

>95%

Expression system

Native

Tags

Tag free

Applications

IHC-P, WB, Dot, IHC-Fr, ELISA

applications

Biologically active

No

Conjugation

Alkaline Phosphatase

Excitation/Emission
Accession

P02976

Animal free

No

Carrier free

No

Species

Staphylococcus aureus subsp. aureus NCTC 8325

Storage buffer

Preservative: 0.1% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1.5% BSA, 0.87% Sodium chloride, 0.79% Tris HCl, 0.0095% Magnesium chloride, 0.00136% Zinc chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Protein A Concentration: 1.0 mg/ml (by UV absorbance at 280 nm) This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the stock concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"56 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Native","expressionSystem":null,"accessionNumber":"P02976","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
False
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Specifications

Form

Liquid

Additional notes

Protein A Alkaline Phosphatase Conjugated was prepared from chromatographically pure Protein A.

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General info

Function

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).

Sequence similarities

Belongs to the immunoglobulin-binding protein SpA family.

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Product protocols

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Target data

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).
See full target information spa
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