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AB7455

Native Staphylococcus aureus Protein A (FITC)

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(2 Publications)

Native Staphylococcus aureus Protein A (FITC) is a Staphylococcus aureus subsp. aureus NCTC 8325 Full Length protein, expressed in Native, with >95%, suitable for Flow Cyt, IM, WB, Dot.

View Alternative Names

SAOUHSC_00069, spa, Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SpA

Key facts

Purity

>95%

Expression system

Native

Tags

Tag free

Applications

WB, IM, Dot, Flow Cyt

applications

Biologically active

No

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Accession

P02976

Animal free

No

Carrier free

No

Species

Staphylococcus aureus subsp. aureus NCTC 8325

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

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Proteins & Peptides

AB52213

Recombinant Protein A + Protein G

SDS-PAGE - Recombinant Protein A + Protein G (AB52213)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB18596

HRP Anti-Protein A antibody

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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/2500", "notes":"<p></p>" }, "IM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Molecular Weight (protein A): 42,000 daltons

Extinction Coefficient: 0.1% at 280 nm is 0.14 (E0.1% of 0.14)

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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"56 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Native","expressionSystem":null,"accessionNumber":"P02976","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
False
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Specifications

Form

Liquid

Additional notes

This product was prepared from chromatographically pure Protein A.

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General info

Function

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).

Sequence similarities

Belongs to the immunoglobulin-binding protein SpA family.

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Product protocols

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Target data

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).
See full target information spa
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Regenerative therapy 25:24-34 PubMed38108043

2023

Injectable phase-separated tetra-armed poly(ethylene glycol) hydrogel scaffold allows sustained release of growth factors to enhance the repair of critical bone defects.

Applications

Unspecified application

Species

Unspecified reactive species

Shant Nepal,Jinyan Si,Shohei Ishikawa,Masaki Nishikawa,Yasuyuki Sakai,Aya M Akimoto,Hiroyuki Okada,Shinsuke Ohba,Ung-Il Chung,Takamasa Sakai,Hironori Hojo

Journal of virology 91: PubMed28381569

2017

Group A Rotaviruses in Chinese Bats: Genetic Composition, Serology, and Evidence for Bat-to-Human Transmission and Reassortment.

Applications

IF

Species

Unspecified reactive species

Biao He,Xiaohong Huang,Fuqiang Zhang,Weilong Tan,Jelle Matthijnssens,Shaomin Qin,Lin Xu,Zihan Zhao,Ling'en Yang,Quanxi Wang,Tingsong Hu,Xiaolei Bao,Jianmin Wu,Changchun Tu
View all publications
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Product promise

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