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AB7456

Native Staphylococcus aureus Protein A (HRP)

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(20 Publications)

Native Staphylococcus aureus Protein A (HRP) is a Full Length S. aureus Protein A (Spa) protein conjugated to Horseradish Peroxidase (HRP) with >95% purity and suitable for western blot, ELISA, IHC, Dot blot and more.The predicted molecular weight of ab7456 protein is 56 kDa.

- Save time and ensure accurate results - use our S. aureus Protein A (HRP) to probe and detect IgG
- Cited in over 15 publications

View Alternative Names

SAOUHSC_00069, spa, Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SpA

Key facts

Purity

>95% SDS-PAGE

Expression system

Native

Tags

Tag free

Applications

IM, WB, Dot, IHC-P, ELISA

applications

Biologically active

No

Conjugation

HRP

Excitation/Emission
Accession

P02976

Animal free

No

Carrier free

No

Species

Staphylococcus aureus subsp. aureus NCTC 8325

Storage buffer

Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

  • 0
  • 0
Proteins & Peptides

AB52213

Recombinant Protein A + Protein G

SDS-PAGE - Recombinant Protein A + Protein G (AB52213)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Primary Antibodies

AB18596

HRP Anti-Protein A antibody

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/40000", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/200000", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"<p></p>" } } }
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Product details

Molecular Weight (protein A): 42,000 daltons

Extinction Coefficient: 0.1% at 280 nm is 0.14 (E0.1% of 0.14)

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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"56 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Native","expressionSystem":null,"accessionNumber":"P02976","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

Protein A Peroxidase Conjugation was prepared from chromatographically pure Protein A.

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General info

Function

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).

Sequence similarities

Belongs to the immunoglobulin-binding protein SpA family.

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Product protocols

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Target data

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).
See full target information spa
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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Life (Basel, Switzerland) 15: PubMed40003558

2025

DYRK1A Up-Regulation Specifically Impairs a Presynaptic Form of Long-Term Potentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Aude-Marie Lepagnol-Bestel,Simon Haziza,Julia Viard,Paul A Salin,Arnaud Duchon,Yann Herault,Michel Simonneau

Small (Weinheim an der Bergstrasse, Germany) 19:e2205819 PubMed36564365

2022

Assembly of Immunogenic Protein Particles toward Advanced Synthetic Vaccines.

Applications

Unspecified application

Species

Unspecified reactive species

Shuxiong Chen,Diana H Quan,Gayathri Sam,Victoria Ozberk,Xiaonan T Wang,Peter Halfmann,Manisha Pandey,Michael F Good,Yoshihiro Kawaoka,Warwick J Britton,Bernd H A Rehm

Life science alliance 5: PubMed35914814

2022

Chr21 protein-protein interactions: enrichment in proteins involved in intellectual disability, autism, and late-onset Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Viard,Yann Loe-Mie,Rachel Daudin,Malik Khelfaoui,Christine Plancon,Anne Boland,Francisco Tejedor,Richard L Huganir,Eunjoon Kim,Makoto Kinoshita,Guofa Liu,Volker Haucke,Thomas Moncion,Eugene Yu,Valérie Hindie,Henri Bléhaut,Clotilde Mircher,Yann Herault,Jean-François Deleuze,Jean-Christophe Rain,Michel Simonneau,Aude-Marie Lepagnol-Bestel

Cell death and differentiation 29:1152-1163 PubMed34839354

2021

ANKRD13a controls early cell-death checkpoint by interacting with RIP1 independent of NF-κB.

Applications

Unspecified application

Species

Unspecified reactive species

Minho Won,Kyeong Ah Park,Sup Kim,Eunjin Ju,Youngbok Ko,Heonjong Yoo,Hyunju Ro,Jaeseob Lee,Junseo Oh,Eun Gyo Lee,Sang Yean Kim,Suk Woo Nam,Han-Ming Shen,Min-Kyung Yeo,Jin Man Kim,Gang Min Hur

Journal of experimental & clinical cancer research 38:370 PubMed31438997

2019

Topoisomerase inhibitors promote cancer cell motility via ROS-mediated activation of JAK2-STAT1-CXCL1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiafei Liu,Like Qu,Lin Meng,Chengchao Shou

Nature communications 10:3218 PubMed31324769

2019

Dopey1-Mon2 complex binds to dual-lipids and recruits kinesin-1 for membrane trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Divyanshu Mahajan,Hieng Chiong Tie,Bing Chen,Lei Lu

Current biology : CB 29:1002-1018.e7 PubMed30853435

2019

Prdm9 and Meiotic Cohesin Proteins Cooperatively Promote DNA Double-Strand Break Formation in Mammalian Spermatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Tanmoy Bhattacharyya,Michael Walker,Natalie R Powers,Catherine Brunton,Alexander D Fine,Petko M Petkov,Mary Ann Handel

The Journal of veterinary medical science 81:328-337 PubMed30587673

2018

Serological and histological evaluation of species-specific immunocontraceptive vaccine antigens based on zona pellucida 3 in the small Indian mongoose (Herpestes auropunctatus).

Applications

Unspecified application

Species

Unspecified reactive species

Naotoshi Kuninaga,Makoto Asano,Ryota Matsuyama,Takahiro Minemoto,Takayuki Mori,Masatsugu Suzuki

Nature communications 9:5182 PubMed30518920

2018

NLRP3 lacking the leucine-rich repeat domain can be fully activated via the canonical inflammasome pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Iva Hafner-Bratkovič,Petra Sušjan,Duško Lainšček,Ana Tapia-Abellán,Kosta Cerović,Lucija Kadunc,Diego Angosto-Bazarra,Pablo Pelegrin,Roman Jerala

Nature communications 9:4987 PubMed30478271

2018

Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Shi,Bing Chen,Divyanshu Mahajan,Boon Kim Boh,Yan Zhou,Bamaprasad Dutta,Hieng Chiong Tie,Siu Kwan Sze,Geng Wu,Lei Lu
View all publications
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Product promise

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