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AB325721

R-CHP Collagen Hybridizing Peptide - Cy3 Conjugate - Scrambled Control

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The Collagen Hybridizing Peptide (CHP) is a synthetic peptide that can specifically bind to denatured collagen strands through hydrogen bonding, both in histology, in vivo, and in vitro (3D cell culture). Additionally, CHP can be used in several biochemical assays, such as SDS-PAGE staining, for identification and quantification of collagen content in a biological sample.

R-CHP is labeled with sulfo-Cyanine3 for direct fluorescence detection.
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Immunofluorescence - R-CHP Collagen Hybridizing Peptide - Cy3 Conjugate - Scrambled Control (AB325721)
  • IF

Supplier Data

Immunofluorescence - R-CHP Collagen Hybridizing Peptide - Cy3 Conjugate - Scrambled Control (AB325721)

Immunofluorescence (IF) analysis of OCT embedded porcine ligament tissues labeled with ab325721 (red) at 20 uM counterstained with DAPI (blue). Tissues were deparaffinized following standard procedures before undergoing heat-induced epitope retrieval (HIER) to purposefully denature all collagen in the sample. This section was incubated over night at 4°C.

Key facts

Purity

>85% HPLC

Tags

Tag free

Biologically active

No

Conjugation

Cy3®

Excitation/Emission

Ex: 552nm, Em: 565nm

Animal free

Yes

Carrier free

No

Storage buffer

Constituents: 100% Proprietary Non-haz Component

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IF": { "reactivity":"TESTED_AND_DOES_NOT_REACT", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab325721 is the negative control for ab325720.

CHP binds to the unfolded triple-helical chains of all collagen types (e.g., I, II, III, IV, etc).

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Sequence info

[{"linker":null,"sequence":null,"proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Synthetic","expressionSystem":null,"accessionNumber":null,"tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
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Specifications

Form

Lyophilized

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General info

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Product protocols

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Target data

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Product promise

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