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AB225972

Recombinant 4,5-DOPA dioxygenase extradiol protein (Tagged)

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Recombinant 4,5-DOPA dioxygenase extradiol protein (Tagged) is a Full Length protein, in the 1 to 271 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

10x His tag N-Terminus SUMO tag N-Terminus Myc tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Animal free

No

Carrier free

No

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGVGKEVSFKESFFLSHGNPAMLADESFIARNFLLGWKKNVFPVKPKSILVVSAHWETDVPCVSAGQYPNVIYDFTEVPASMFQMKYPAPGCPKLAKRVQELLIAGGFKSAKLDEERGFDHSSWVPLSMMCPEADIPVCQLSVQPGLDATHHFNVGRALAPLKGEGVLFIGSGGAVHPSDDTPHWFDGVAPWAAEFDQWLEDALLEGRYEDVNNYQTKAPEGWKLAHPIPEHFLPLHVAMGAGGEKSKAELIYRTWDHGTLGYASYKFTSI","proteinLength":"Full Length","predictedMolecularWeight":"49.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":271,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":null,"tags":[{"tag":"10x His","terminus":"N-Terminus"},{"tag":"SUMO","terminus":"N-Terminus"},{"tag":"Myc","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

45-DOPA dioxygenase extradiol is an enzyme that performs a specific role in breaking down certain aromatic compounds. This enzyme sometimes referred to as "DOPA dioxygenase" facilitates the extradiol cleavage of catecholic compounds introducing oxygen into specific bonds of the substrate. Its molecular weight is approximately 32 kDa. Expression of 45-DOPA dioxygenase extradiol primarily occurs in microorganisms such as soil bacteria where it aids in the degradation of complex organic materials.
Biological function summary

The action of 45-DOPA dioxygenase extradiol contributes significantly to the metabolism of aromatic hydrocarbons. It is not typically associated with large protein complexes but acts independently to influence the cellular processes of its host organism. Through its enzymatic reaction it helps manage oxidative stress within bacteria by converting potentially toxic compounds into forms that can be further metabolized and eliminated.

Pathways

45-DOPA dioxygenase extradiol plays a role in the aromatic ring-cleavage pathway which is key to the biodegradation of pollutants. This pathway is essential for the bacterial conversion of toxic aromatic hydrocarbons into non-toxic compounds that can be integrated into central metabolic pathways. This enzyme works together with other proteins like catechol 23-dioxygenase in these degradation processes ultimately promoting environmental detoxification.

45-DOPA dioxygenase extradiol itself is not directly linked to human conditions but its function in bacterial metabolism can impact processes related to environmental health. The enzyme’s activity in degrading pollutants may help in reducing the risk of exposure-related health issues like respiratory disorders. Additionally by modulating bacterial populations that interact with human health proteins related to this enzyme's pathways such as catechol 12-dioxygenase can indirectly affect biomedical environments.
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Specifications

Form

Liquid

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General info

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Product protocols

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Target data

See full target information 4,5-DOPA dioxygenase extradiol
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Product promise

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