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Recombinant A. victoria GFP protein is a Aequorea victoria Full Length protein, in the 1 to 238 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, WB, FuncS.
Alternative names=Green fluorescent protein, GFP
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, WB, FuncS
- Biologically active
Yes
- Sequence
M S K G E E L F T G V V P I L V E L D G D V N G H K F S V S G E G E G D A T Y G K L T L K F I C T T G K L P V P W P T L V T T F S Y G V Q C F S R Y P D H M K Q H D F F K S A M P E G Y V Q E R T I F F K D D G N Y K T R A E V K F E G D T L V N R I E L K G I D F K E D G N I L G H K L E Y N Y N S H N V Y I M A D K Q K N G I K V N F K I R H N I E D G S V Q L A D H Y Q Q N T P I G D G P V L L P D N H Y L S T Q S A L S K D P N E K R D H M V L L E F V T A A G I T H G M D E L Y K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes Ab84191 can be used as a WB positive control in conjunction with ab290. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Images
Target data
Function
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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Recombinant A. victoria GFP protein is a Aequorea victoria Full Length protein, in the 1 to 238 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, WB, FuncS.
Alternative names=Green fluorescent protein, GFP
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, WB, FuncS
- Biologically active
Yes
- Biological activity
Active
- Predicted molecular weight
26.8 kDa
- Amino acids
1 to 238
- Sequence
M S K G E E L F T G V V P I L V E L D G D V N G H K F S V S G E G E G D A T Y G K L T L K F I C T T G K L P V P W P T L V T T F S Y G V Q C F S R Y P D H M K Q H D F F K S A M P E G Y V Q E R T I F F K D D G N Y K T R A E V K F E G D T L V N R I E L K G I D F K E D G N I L G H K L E Y N Y N S H N V Y I M A D K Q K N G I K V N F K I R H N I E D G S V Q L A D H Y Q Q N T P I G D G P V L L P D N H Y L S T Q S A L S K D P N E K R D H M V L L E F V T A A G I T H G M D E L Y K
- Accession
- P42212-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Aequorea victoria
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl
Specifications
- Form
Liquid
- Additional notes
Purified using conventional chromatography techniques.
General info
- Function
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
- Sequence similarities
Belongs to the GFP family.
- Post-translational modifications
Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Endotoxin Level determined by LAL method.
Concentration determined by Bradford assay.
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