Recombinant A. victoria GFP protein

• WB

Supplier Data

Western blot - Recombinant A. victoria GFP protein (AB84191)

**Block : ** Blocking buffer for 30 minutes at RT.

All lanes:

Western blot - FITC Anti-GFP antibody (<a href='/en-us/products/primary-antibodies/fitc-gfp-antibody-ab6662'>ab6662</a>)

All lanes:

Western blot - Recombinant A. victoria GFP protein (ab84191)

Secondary

All lanes:

Fluorescein goat secondary antibody for 60 minutes at RT at 1/1000 dilution

Predicted band size: 27 kDa

false

• FuncS

Unknown

Functional Studies - Recombinant A. victoria GFP protein (AB84191)

1. Prepare a 150 μl recombinant GFP with various concentrations (0.00074 nM - 6.21 μM) in assay buffer and equilibrate to 25°C. (Assay buffer : 10 mM Tris-HCl (pH 8.0), 10 mM EDTA, 0.02% sodium azide.)

2. Read at excitation wavelengths 485 nm and emission 535 nm.

- 96 Well Polystyrene Microplate, black

- Fluorescent plate reader

• WB

Lab

Western blot - Recombinant A. victoria GFP protein (AB84191)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab190584 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-GFP antibody [E385] (<a href='/en-us/products/primary-antibodies/hrp-gfp-antibody-e385-ab190584'>ab190584</a>) at 1/10000 dilution

Lane 1:

Western blot - Recombinant A. victoria GFP protein (ab84191) at 0.1 µg

Lane 2:

Western blot - Recombinant RFP protein (<a href='/en-us/products/proteins-peptides/recombinant-rfp-protein-ab51993'>ab51993</a>) at 0.1 µg

Predicted band size: 27 kDa

Observed band size: 27 kDa

true

Exposure time: 10s

• WB

Lab

Western blot - Recombinant A. victoria GFP protein (AB84191)

Secondary antibody - goat anti-rabbit HRP preadsorbed (ab97080)

All lanes:

Western blot - Anti-GFP antibody (<a href='/en-us/products/primary-antibodies/gfp-antibody-ab290'>ab290</a>) at 1/2500 dilution

All lanes:

Western blot - Recombinant A. victoria GFP protein (ab84191) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

true

Exposure time: 30s

• SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant A. victoria GFP protein (AB84191)

3 ug of reduced ab84191 on SDS-PAGE, stained with Coomassie Blue.