Recombinant A. victoria GFP protein is a Aequorea victoria Full Length protein, in the 1 to 238 aa range with >95% purity, < 1 EU/µg endotoxin level and suitable for western blot, Functional studies and SDS-PAGE. The predicted molecular weight of ab84191 recombinant protein is 26.8 kDa.
- Save time and ensure accurate results - use our recombinant GFP protein as a control
- Available in different sizes to fit your experimental needs
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- Science advances 9:eadg18402023IGFBP2 secretion by mammary adipocytes limits breast cancer invasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames R W Conway,Defne D Dinç,Gautier Follain,Oona Paavolainen,Jasmin Kaivola,Pia Boström,Pauliina Hartiala,Emilia Peuhu,Johanna IvaskaPubMed 37436978
- Journal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience 21:A133-A1412023An Undergraduate Laboratory Series Using That Prepares Students for Independent Inquiry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVirginia Quinan,Kelly Hsu,Molly Mann,Kia Barclay,Deborah BauerPubMed 37588649
- The Journal of clinical investigation 132:2022Immune tolerance against infused FVIII in hemophilia A is mediated by PD-L1+ Tregs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJanine Becker-Gotot,Mirjam Meissner,Vadim Kotov,Blanca Jurado-Mestre,Andrea Maione,Andreas Pannek,Thilo Albert,Chrystel Flores,Frank A Schildberg,Paul A Gleeson,Birgit M Reipert,Johannes Oldenburg,Christian KurtsPubMed 36107620
- Nature communications 13:63752022Ultrafast one-minute electronic detection of SARS-CoV-2 infection by 3CL enzymatic activity in untreated saliva samples.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElla Borberg,Eran Granot,Fernando PatolskyPubMed 36289211
- ACS nano 16:13800-138132022The "Bloodless" Blood Test: Intradermal Prick Nanoelectronics for the Blood Extraction-Free Multiplex Detection of Protein Biomarkers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNimrod Harpak,Ella Borberg,Adva Raz,Fernando PatolskyPubMed 36006419
- Proceedings of the National Academy of Sciences of the United States of America 118:2021An ELISA-based method for rapid genetic screens in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaylor R Jay,Yunsik Kang,Amanda Jefferson,Marc R FreemanPubMed 34686600
- EMBO reports 21:e497352020G-Quadruplexes act as sequence-dependent protein chaperones.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdam Begeman et. al.PubMed 32945124
- eLife 7:2018Loss of Fam60a, a Sin3a subunit, results in embryonic lethality and is associated with aberrant methylation at a subset of gene promoters.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyo Nabeshima et. al.PubMed 30070635
- Nature communications 9:15612018Gold-nanofève surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMegumi Shiota et. al.PubMed 29674746
- The Journal of neuroscience : the official journal of the Society for Neuroscience 38:2341-23582018Progranulin Gene Therapy Improves Lysosomal Dysfunction and Microglial Pathology Associated with Frontotemporal Dementia and Neuronal Ceroid Lipofuscinosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew E Arrant,Vincent C Onyilo,Daniel E Unger,Erik D RobersonPubMed 29378861
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Amino acid sequence
M S K G E E L F T G V V P I L V E L D G D V N G H K F S V S G E G E G D A T Y G K L T L K F I C T T G K L P V P W P T L V T T F S Y G V Q C F S R Y P D H M K Q H D F F K S A M P E G Y V Q E R T I F F K D D G N Y K T R A E V K F E G D T L V N R I E L K G I D F K E D G N I L G H K L E Y N Y N S H N V Y I M A D K Q K N G I K V N F K I R H N I E D G S V Q L A D H Y Q Q N T P I G D G P V L L P D N H Y L S T Q S A L S K D P N E K R D H M V L L E F V T A A G I T H G M D E L Y K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab84191 can be used as a WB positive control in conjunction with Anti-GFP antibody ab290. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Alternative names
Green fluorescent protein, GFP
Recommended products
Recombinant A. victoria GFP protein is a Aequorea victoria Full Length protein, in the 1 to 238 aa range with >95% purity, < 1 EU/µg endotoxin level and suitable for western blot, Functional studies and SDS-PAGE. The predicted molecular weight of ab84191 recombinant protein is 26.8 kDa.
- Save time and ensure accurate results - use our recombinant GFP protein as a control
- Available in different sizes to fit your experimental needs
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- Active
- Accession
- P42212-1
- Animal free
- No
- Species
- Aequorea victoria
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl
Sequence info
Amino acid sequence
- M S K G E E L F T G V V P I L V E L D G D V N G H K F S V S G E G E G D A T Y G K L T L K F I C T T G K L P V P W P T L V T T F S Y G V Q C F S R Y P D H M K Q H D F F K S A M P E G Y V Q E R T I F F K D D G N Y K T R A E V K F E G D T L V N R I E L K G I D F K E D G N I L G H K L E Y N Y N S H N V Y I M A D K Q K N G I K V N F K I R H N I E D G S V Q L A D H Y Q Q N T P I G D G P V L L P D N H Y L S T Q S A L S K D P N E K R D H M V L L E F V T A A G I T H G M D E L Y K
- Accession
- P42212
- Protein length
- Full Length
- Predicted molecular weight
- 26.8 kDa
- Amino acids
- 1 to 238
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Purified using conventional chromatography techniques.
General info
- Function
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
- Sequence similarities
Belongs to the GFP family.
- Post-translational modifications
Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Ensure the validity of your result using our Recombinant A. victoria GFP protein ab84191 as a control. ab84191 can be used as a positive control in SDS-PAGE and western blot assays.
Check out our Western blot protocol for more information here
Endotoxin Level determined by LAL method.
Concentration determined by Bradford assay.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Western blot - Recombinant A. victoria GFP protein (ab84191)
**Block:** Blocking buffer for 30 minutes at RT.
All lanes: Western blot - FITC Anti-GFP antibody (FITC Anti-GFP antibody ab6662)
All lanes: Western blot - Recombinant A. victoria GFP protein (ab84191)
SecondaryAll lanes: Fluorescein goat secondary antibody for 60 minutes at RT at 1/1000 dilution
Predicted band size: 27 kDa
Western blot - Recombinant A. victoria GFP protein (ab84191)
Secondary antibody - goat anti-rabbit HRP preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080)
All lanes: Western blot - Anti-GFP antibody (Anti-GFP antibody ab290) at 1/2500 dilution
All lanes: Western blot - Recombinant A. victoria GFP protein (ab84191) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 30s
Western blot - Recombinant A. victoria GFP protein (ab84191)
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-GFP antibody [E385] ab190584 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - HRP Anti-GFP antibody [E385] (HRP Anti-GFP antibody [E385] ab190584) at 1/10000 dilution
Lane 1: Western blot - Recombinant A. victoria GFP protein (ab84191) at 0.1 µg
Lane 2: Western blot - Recombinant RFP protein (Recombinant RFP protein ab51993) at 0.1 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 10s
Functional Studies - Recombinant A. victoria GFP protein (ab84191)
1. Prepare a 150 μl recombinant GFP with various concentrations (0.00074 nM - 6.21 μM) in assay buffer and equilibrate to 25°C. (Assay buffer: 10 mM Tris-HCl (pH 8.0), 10 mM EDTA, 0.02% sodium azide.)
2. Read at excitation wavelengths 485 nm and emission 535 nm.
- 96 Well Polystyrene Microplate, black
- Fluorescent plate reader
SDS-PAGE - Recombinant A. victoria GFP protein (ab84191)
3 ug of reduced ab84191 on SDS-PAGE, stained with Coomassie Blue.
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