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AB286018

Recombinant Acidaminococcus fermentans D2HGDH protein (Active)

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Recombinant Acidaminococcus fermentans D2HGDH protein (Active) is a Acidaminococcus fermentans DSM 20731 Full Length protein, expressed in Escherichia coli, with >99%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Acfer_0976, hgdH, (R)-2-hydroxyglutarate dehydrogenase, HGDH

2 Images
SDS-PAGE - Recombinant Acidaminococcus fermentans D2HGDH protein (Active) (AB286018)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Acidaminococcus fermentans D2HGDH protein (Active) (AB286018)

Active D2HGDH reacts with D2HG specifically. 1 ng D2HGDH reacts with 2~10 nmole D2HG. Activity can be calculated from the increase of OD 450 nm after 1 hour incubation at 37°C using the D-2-Hydroxyglutarate Colorimetric Assay Kit.

SDS-PAGE - Recombinant Acidaminococcus fermentans D2HGDH protein (Active) (AB286018)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Acidaminococcus fermentans D2HGDH protein (Active) (AB286018)

SDS-PAGE (4-20%) of Active D2HGDH (ab286018) :

Recombinant D2HGDH Protein loaded under reducing conditions and stained with Coomassie Blue. The protein has a predicted MW of 39 kDa.

Key facts

Purity

>99% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of D2HGDH is ≥ 40,000 mU/mg.

Accession

D2RJU7

Animal free

Yes

Carrier free

No

Species

Acidaminococcus fermentans DSM 20731

Storage buffer

pH: 7.4 Constituents: 10.269% Trehalose, 1% Potassium phosphate monobasic

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

This product is manufactured by BioVision, an Abcam company and was previously called P1001 Active D-2-Hydroxyglutaratate Dehydrogenase (D2HGDH), Recombinant. P1001-50 is the same size as the 50 μg size of ab286018.

Sequence info

[{"sequence":"MKVLCYGVRDVELPIFEACNKEFGYDIKCVPDYLNTKETAEMAAGFDAVILRGNCFANKQNLDIYKKLGVKYILTRTAGTDHIDKEYAKELGFPMAFVPRYSPNAIAELAVTQAMMLLRHTAYTTSRTAKKNFKVDAFMFSKEVRNCTVGVVGLGRIGRVAAQIFHGMGATVIGEDVFEIKGIEDYCTQVSLDEVLEKSDIITIHAPYIKENGAVVTRDFLKKMKDGAILVNCARGQLVDTEAVIEAVESGKLGGYGCDVLDGEASVFGKDLEGQKLENPLFEKLVDLYPRVLITPHLGSYTDEAVKNMVEVSYQNLKDLAETGDCPNKIKMGSSHHHHHHSSGLVPRGSHM","proteinLength":"Full Length","predictedMolecularWeight":"39 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"D2RJU7","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
True

Specifications

Form

Lyophilized

General info

Function

Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate.

Sequence similarities

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Product protocols

Target data

Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate.
See full target information hgdH

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