Recombinant Adamalysin 2 protein (His tag) is a Eastern Diamondback Rattlesnake Full Length protein, in the 1 to 203 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
Q Q N L P Q R Y I E L V V V A D R R V F M K Y N S D L N I I R T R V H E I V N I I N G F Y R S L N I D V S L V N L E I W S G Q D P L T I Q S S S S N T L N S E G L W R E K V L L N K K K K D N A Q L L T A I E F K C E T L G K A Y L N S M C N P R S S V G I V K D H S P I N L L V A V T M A H E L G H N L G M E H D G K D C L R G A S L C I M R P G L T P G R S Y E F S D D S M G Y Y Q K F L N Q Y K P Q C I L N K P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Has no significant hemorrhagic activity, but inactivates serpins by limited proteolysis of their reactive-site loops.
Alternative names
Snake venom metalloproteinase adamalysin-2, SVMP, Adamalysin II, Proteinase II
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Recombinant Adamalysin 2 protein (His tag) is a Eastern Diamondback Rattlesnake Full Length protein, in the 1 to 203 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P34179-1
- Animal free
No
- Carrier free
Yes
- Species
Eastern Diamondback Rattlesnake
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
Q Q N L P Q R Y I E L V V V A D R R V F M K Y N S D L N I I R T R V H E I V N I I N G F Y R S L N I D V S L V N L E I W S G Q D P L T I Q S S S S N T L N S E G L W R E K V L L N K K K K D N A Q L L T A I E F K C E T L G K A Y L N S M C N P R S S V G I V K D H S P I N L L V A V T M A H E L G H N L G M E H D G K D C L R G A S L C I M R P G L T P G R S Y E F S D D S M G Y Y Q K F L N Q Y K P Q C I L N K P
- Accession
- Protein length
Full Length
- Predicted molecular weight
27.1 kDa
- Amino acids
1 to 203
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Has no significant hemorrhagic activity, but inactivates serpins by limited proteolysis of their reactive-site loops.
- Sequence similarities
Belongs to the venom metalloproteinase (M12B) family. P-I subfamily.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Adamalysin 2 also known as ADAM2 or fertilin beta operates as a metalloprotease and disintegrin. This protein plays roles in cell adhesion and proteolytic processing. ADAM2 has a mass of approximately 84 kDa and is predominantly expressed in testicular tissues where it facilitates sperm-egg interactions. By contributing to cell membrane modifications ADAM2 assists in important reproductive processes.
- Biological function summary
Adamalysin 2 functions within the reproductive system aiding in gamete fusion and sperm maturation. This protein participates as a component of the ADAM family which includes both membrane-anchored proteins and soluble members. ADAM2's proteolytic activity facilitates cleavage of extracellular matrix components therefore regulating cellular environments and matrix remodeling events particularly during fertilization.
- Pathways
Adamalysin 2 integrates into cellular adhesion and matrix degradation pathways. It plays an important role in the fertilization process involving the binding and fusion of sperm and egg membranes. More specifically ADAM2 engages with other proteins like ADAM1 and integrins performing functions critical for efficient sperm-egg recognition and fusion within the fertilization pathway.
- Associated diseases and disorders
Adamalysin 2 has connections to infertility issues particularly in male reproductive health. Inadequate expression or mutations within the ADAM2 gene can lead to impaired sperm function and subfertility. Related proteins such as ADAM1 and integrins may also be implicated due to their interconnected roles in ensuring proper gametic interaction and successful reproduction.
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1 product image
SDS-PAGE - Recombinant Adamalysin 2 protein (His tag) (ab237551)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab237551.
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