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AB225976

Recombinant Adenylate kinase protein (Tagged)

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Recombinant Adenylate kinase protein (Tagged) is a Shigella flexneri Full Length protein, in the 1 to 214 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

SF0419, S0426, adk, Adenylate kinase, AK, ATP-AMP transphosphorylase, ATP:AMP phosphotransferase, Adenylate monophosphate kinase

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

10x His tag N-Terminus SUMO tag N-Terminus Myc tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q83M40

Animal free

No

Carrier free

No

Species

Shigella flexneri

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB243636

Anti-ADK antibody [EPR23166-143]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADK antibody [EPR23166-143] (AB243636)

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Proteins & Peptides

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Recombinant human Apolipoprotein E3

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Proteins & Peptides

AB7403

Streptavidin (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin (HRP) (AB7403)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSELGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGINVDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQEETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG","proteinLength":"Full Length","predictedMolecularWeight":"43.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":214,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q83M40","tags":[{"tag":"10x His","terminus":"N-Terminus"},{"tag":"SUMO","terminus":"N-Terminus"},{"tag":"Myc","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adenylate kinase also known by its alternate names AK or myokinase is an important enzyme that catalyzes the conversion of two molecules of ADP into one molecule of ATP and one molecule of AMP. This process is often referred to as the adenylate kinase reaction. The enzyme has a molecular mass of approximately 23-25 kDa. Adenylate kinase is expressed in almost all types of cells with particularly high expression in metabolically active tissues such as the heart muscle and brain.
Biological function summary

Adenylate kinase plays a fundamental role in maintaining cellular energy homeostasis by regulating the levels of adenine nucleotides within the cell. This enzyme is typically found in multiple isoforms which are localized in different cellular compartments allowing fine regulatory control over cellular energy states. It is not known to operate as part of a larger complex but its function is essential for numerous cellular processes that require precise energy management.

Pathways

Adenylate kinase is a pivotal component of the purine nucleotide cycle and the energy transfer cycle. In the purine nucleotide cycle it collaborates with enzymes like AMP deaminase and adenylosuccinate synthetase ensuring balance and replenishment of energy supplies. The enzyme also plays a critical role in the phosphotransfer network working alongside creatine kinase and glycolysis-related enzymes to facilitate efficient energy transfer within cells and tissues.

Adenylate kinase has relevance to mitochondrial diseases and cardiac dysfunction. Deficiencies or mutations in adenylate kinase can lead to disturbances in cellular energy homeostasis contributing to conditions such as muscular dystrophy and neurodegenerative diseases. The enzyme interacts with other mitochondrial proteins such as ATP synthase and cytochrome c oxidase highlighting its role in energy metabolism disorders.
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Specifications

Form

Liquid

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General info

Function

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.

Sequence similarities

Belongs to the adenylate kinase family.

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Product protocols

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Target data

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.
See full target information adk
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