Recombinant Adenylate kinase protein (Tagged) is a Shigella flexneri Full Length protein, in the 1 to 214 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
>90% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE
No
M R I I L L G A P G A G K G T Q A Q F I M E K Y G I P Q I S T G D M L R A A V K S G S E L G K Q A K D I M D A G K L V T D E L V I A L V K E R I A Q E D C R N G F L L D G F P R T I P Q A D A M K E A G I N V D Y V L E F D V P D E L I V D R I V G R R V H A P S G R V Y H V K F N P P K V E G K D D V T G E E L T T R K D D Q E E T V R K R L V E Y H Q M T A P L I G Y Y S K E A E A G N T K Y A K V D G T K Q V A E V R A D L E K I L G
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.
Adenylate kinase, AK, ATP-AMP transphosphorylase, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, S0426, adk, SF0419
Recombinant Adenylate kinase protein (Tagged) is a Shigella flexneri Full Length protein, in the 1 to 214 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Adenylate kinase, AK, ATP-AMP transphosphorylase, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, S0426, adk, SF0419
>90% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE
No
No
Shigella flexneri
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
M R I I L L G A P G A G K G T Q A Q F I M E K Y G I P Q I S T G D M L R A A V K S G S E L G K Q A K D I M D A G K L V T D E L V I A L V K E R I A Q E D C R N G F L L D G F P R T I P Q A D A M K E A G I N V D Y V L E F D V P D E L I V D R I V G R R V H A P S G R V Y H V K F N P P K V E G K D D V T G E E L T T R K D D Q E E T V R K R L V E Y H Q M T A P L I G Y Y S K E A E A G N T K Y A K V D G T K Q V A E V R A D L E K I L G
Full Length
43.6 kDa
1 to 214
Recombinant
His tag N-Terminus
Liquid
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.
Belongs to the adenylate kinase family.
Blue Ice
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Adenylate kinase also known by its alternate names AK or myokinase is an important enzyme that catalyzes the conversion of two molecules of ADP into one molecule of ATP and one molecule of AMP. This process is often referred to as the adenylate kinase reaction. The enzyme has a molecular mass of approximately 23-25 kDa. Adenylate kinase is expressed in almost all types of cells with particularly high expression in metabolically active tissues such as the heart muscle and brain.
Adenylate kinase plays a fundamental role in maintaining cellular energy homeostasis by regulating the levels of adenine nucleotides within the cell. This enzyme is typically found in multiple isoforms which are localized in different cellular compartments allowing fine regulatory control over cellular energy states. It is not known to operate as part of a larger complex but its function is essential for numerous cellular processes that require precise energy management.
Adenylate kinase is a pivotal component of the purine nucleotide cycle and the energy transfer cycle. In the purine nucleotide cycle it collaborates with enzymes like AMP deaminase and adenylosuccinate synthetase ensuring balance and replenishment of energy supplies. The enzyme also plays a critical role in the phosphotransfer network working alongside creatine kinase and glycolysis-related enzymes to facilitate efficient energy transfer within cells and tissues.
Adenylate kinase has relevance to mitochondrial diseases and cardiac dysfunction. Deficiencies or mutations in adenylate kinase can lead to disturbances in cellular energy homeostasis contributing to conditions such as muscular dystrophy and neurodegenerative diseases. The enzyme interacts with other mitochondrial proteins such as ATP synthase and cytochrome c oxidase highlighting its role in energy metabolism disorders.
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