Recombinant Angiotensinogen (AGT) is a Human Fragment protein, in the 35 to 485 aa range, expressed in Escherichia coli, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
R V Y I H P F H L V I H N E S T C E Q L A K A N A G K P K D P T F I P A P I Q A K T S P V D E K A L Q D Q L V L V A A K L D T E D K L R A A M V G M L A N F L G F R I Y G M H S E L W G V V H G A T V L S P T A V F G T L A S L Y L G A L D H T A D R L Q A I L G V P W K D K N C T S R L D A H K V L S A L Q A V Q G L L V A Q G R A D S Q A Q L L L S T V V G V F T A P G L H L K Q P F V Q G L A L Y T P V V L P R S L D F T E L D V A A E K I D R F M Q A V T G W K T G C S L M G A S V D S T L A F N T Y V H F Q G K M K G F S L L A E P Q E F W V D N S T S V S V P M L S G M G T F Q H W S D I Q D N F S V T Q V P F T E S A C L L L I Q P H Y A S D L D K V E G L T F Q Q N S L N W M K K L S P R T I H L T M P Q L V L Q G S Y D L Q D L L A Q A E L P A I L H T E L N L Q K L S N D R I R V G E V L N S I F F E L E A D E R E P T E S T Q Q L N K P E V L E V T L N R P F L F A V Y D Q S A T A L H F L G R V A N P L S T A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. Angiotensin-2. Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone (PubMed:10619573, PubMed:17138938). Acts by binding to angiotensin receptors AGTR1 and AGTR2 (PubMed:1567413). Also binds the DEAR/FBXW7-AS1 receptor (By similarity). Angiotensin-3. Stimulates aldosterone release. Angiotensin 1-7. Is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets (By similarity).
Alternative names
SERPINA8, AGT, Angiotensinogen, Serpin A8
Recommended products
Recombinant Angiotensinogen (AGT) is a Human Fragment protein, in the 35 to 485 aa range, expressed in Escherichia coli, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P01019-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
Preservative: 0.82% Proclin 300
Constituents: 81.9% Trehalose, 16.2% PBS, 0.25% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.16% Sodium-N-Lauroylsarcosinate
Sequence info
Amino acid sequence
- R V Y I H P F H L V I H N E S T C E Q L A K A N A G K P K D P T F I P A P I Q A K T S P V D E K A L Q D Q L V L V A A K L D T E D K L R A A M V G M L A N F L G F R I Y G M H S E L W G V V H G A T V L S P T A V F G T L A S L Y L G A L D H T A D R L Q A I L G V P W K D K N C T S R L D A H K V L S A L Q A V Q G L L V A Q G R A D S Q A Q L L L S T V V G V F T A P G L H L K Q P F V Q G L A L Y T P V V L P R S L D F T E L D V A A E K I D R F M Q A V T G W K T G C S L M G A S V D S T L A F N T Y V H F Q G K M K G F S L L A E P Q E F W V D N S T S V S V P M L S G M G T F Q H W S D I Q D N F S V T Q V P F T E S A C L L L I Q P H Y A S D L D K V E G L T F Q Q N S L N W M K K L S P R T I H L T M P Q L V L Q G S Y D L Q D L L A Q A E L P A I L H T E L N L Q K L S N D R I R V G E V L N S I F F E L E A D E R E P T E S T Q Q L N K P E V L E V T L N R P F L F A V Y D Q S A T A L H F L G R V A N P L S T A
- Accession
- P01019
- Protein length
- Fragment
- Predicted molecular weight
- 80 kDa
- Amino acids
- 35 to 485
- Nature
- Recombinant
- Tags
- His tag N-Terminus, GST tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.
- Sequence similarities
Belongs to the serpin family.
- Post-translational modifications
Beta-decarboxylation of Asp-25 in angiotensin-2, by mononuclear leukocytes produces alanine (PubMed:17138938). The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor (PubMed:17138938).
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage duration
- 1 month
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Angiotensinogen also known as AGT is a precursor to angiotensin peptides and plays a mechanical role in the renin-angiotensin system. It is a glycoprotein with a mass of approximately 60 kDa and is mainly produced in the liver. This protein circulates in the bloodstream where it waits for renin to cleave it. Angiotensinogen's presence is significant in various tissues but primarily it functions within the plasma after hepatic secretion.
- Biological function summary
Angiotensinogen is critical in the regulation of blood pressure and fluid balance. It serves as the substrate for renin an enzyme that initiates a cascade leading to the production of angiotensin I which further converts into active forms like angiotensin II. Angiotensinogen does not operate as part of a larger protein complex but interacts closely with enzymes and other substrates to mediate its effects on the cardiovascular system.
- Pathways
Angiotensinogen is an essential component of the renin-angiotensin-aldosterone system (RAAS) which helps regulate blood pressure and electrolyte balance. The pathway involves the conversion of angiotensinogen to angiotensin I by renin followed by the conversion to angiotensin II by angiotensin-converting enzyme (ACE). This system also has connections with proteins such as aldosterone which is involved in sodium and water retention influencing cardiovascular health.
- Associated diseases and disorders
Angiotensinogen links to hypertension and cardiovascular diseases. The protein's interaction with renin and ACE results in the formation of angiotensin II a potent vasoconstrictor that increases blood pressure. Elevated levels of angiotensinogen are often observed in individuals with high 117/85 blood pressure indicating a risk for chronic conditions. Managing angiotensinogen levels through RAAS modulation is a therapeutic strategy for controlling these disorders.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Angiotensinogen (AGT) (ab316920)
Recombinant Angiotensinogen Human, 15% SDS PAGE. Predicted Molecular Mass: 76.7kDa.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com