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AB204629

Recombinant Arginine deiminase protein

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Recombinant Arginine deiminase protein is a Mycoplasma arginini Full Length protein, in the 2 to 410 aa range, expressed in Escherichia coli, with >97%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, HPLC.

View Alternative Names

Arginine deiminase, ADI, Arginine dihydrolase, AD, arcA

Key facts

Purity

>97% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, HPLC, FuncS

applications

Biologically active

Yes

Biological activity

Measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/ml.

Accession

P23793

Animal free

No

Carrier free

No

Species

Mycoplasma arginini

Reconstitution

Reconstitute in water

Storage buffer

Constituents: 0.03278% Sodium phosphate, 0.00308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"SVFDSKFKGIHVYSEIGELESVLVHEPGREIDYITPARLDELLFSAILESHDARKEHKQFVAELKANDINVVELIDLVAETYDLASQEAKDKLIEEFLEDSEPVLSEEHKVVVRNFLKAKKTSRELVEIMMAGITKTDLGIEADHELIVDPMPNLYFTRDPFASVGNGVTIHYMRYKVRQRETLFSRFVFSNHPKLINTPWYYDPSLKLSIEGGDVFIYNNDTLVVGVSERTDLQTVTLLAKNIVANKECEFKRIVAINVPKWTNLMHLDTWLTMLDKDKFLYSPIANDVFKFWDYDLVNGGAEPQPVENGLPLEGLLQSIINKKPVLIPIAGEGASQMEIERETHFDGTNYLAIRPGVVIGYSRNEKTNAALEAAGIKVLPFHGNQLSLGMGNARCMSMPLSRKDVKW","proteinLength":"Full Length","predictedMolecularWeight":"46.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":410,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P23793","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Arginine deiminase also known as protein arginine deiminase or deiminase is an enzyme that converts arginine into citrulline and ammonia by deimidation. The enzyme shows molecular mass around 46 kDa. It can be expressed in a variety of tissues with significant presence observed in bacterial species and in several types of human cells. This enzyme plays an important role in regulating the availability of arginine which is essential for protein synthesis and other metabolic processes.
Biological function summary

The regulation of arginine availability has far-reaching effects on cellular functions. Arginine deiminase takes part in modulating immune responses and can influence nitric oxide production essential in inflammation and cell signaling. This enzyme often functions within a larger enzymatic complex. Its activity helps control cellular proliferation and has implications in both energy metabolism and immune modulation.

Pathways

Converting arginine has significant impact on metabolic and immune pathways. Arginine deiminase has been linked to the urea cycle influencing nitrogen excretion and metabolism. Moreover the enzyme aligns with pathways involved in mTOR signaling a pathway important for cell growth and proliferation. Arginase is another protein associated within these pathways often acting in concert with arginine deiminase to regulate arginine levels.

The role of arginine deiminase relates closely to cancer and immune system dysfunctions. In cancer particularly hepatocellular carcinoma the enzyme's ability to deplete arginine can starve tumor cells reducing their proliferation. The enzyme also connects with immune checkpoints influencing disorders like rheumatoid arthritis by regulating inflammation. Protein interactions with ornithine another amino acid derivative through arginase activity also play role in these diseases.
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Specifications

Form

Lyophilized

Additional notes

>97 % by HPLC.

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General info

Sequence similarities

Belongs to the arginine deiminase family.

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Product protocols

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Target data

See full target information arcA

Additional targets

Arginine deiminase
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