Recombinant B. subtilis Penicillin-binding protein 4 (His tag) is a Bacillus subtilis subsp. subtilis str. 168 Full Length protein, in the 1 to 451 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M K Q N K R K H L Q T L F E T L G E K H Q F N G T V L A A E G G D I L Y H H S F G Y A E M T E K R P L K T N S L F E L A S L S K P F T A L G I I L L E E K G I L G Y E D K V D R W L P G F P Y Q G V T I R H L L N H T S G L P D Y M G W F F A N W D S H K I A V N Q D I V D M L M N E G L S G Y F E P N E G W M Y S N T G Y V L L A V I I E K A S G M S Y A D F I K T S I F L P A G M N E T R V Y N R R L S P E R I D H Y A Y G Y V Y D V H S E T Y V L P D E L E E T N Y V V Y L D G I Q G D G T V N S V T S D L F R F D Q A L Y Q D D F I S K A S K E S A F S P V R L N N G E T I D Y G F G W V L Q N S P E K G R I V S H S G G W P G Y S T M M I R Y I D H R K T L I Y L S N K E E D T E Y E Q A I L K A A E H I L F G Q P Y D V P E R P A D K K K K A I D T A I Y S R Y V G S Y L L Q D G T A A Q V T T E N E R L Y L E I A G Q L R L E L F P S S E T R F F L R A L S V E V E F T L G E D A A K S F I L Y E D G S E E E A V R T K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Probably involved in peptidoglycan modification during cortex synthesis.
Alternative names
Penicillin-binding protein 4*, PBP 4*, PBP 4A, Penicillin-binding protein E, BSU34440, pbpE
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Recombinant B. subtilis Penicillin-binding protein 4 (His tag) is a Bacillus subtilis subsp. subtilis str. 168 Full Length protein, in the 1 to 451 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P32959-1
- Animal free
No
- Species
Bacillus subtilis subsp. subtilis str. 168
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
M K Q N K R K H L Q T L F E T L G E K H Q F N G T V L A A E G G D I L Y H H S F G Y A E M T E K R P L K T N S L F E L A S L S K P F T A L G I I L L E E K G I L G Y E D K V D R W L P G F P Y Q G V T I R H L L N H T S G L P D Y M G W F F A N W D S H K I A V N Q D I V D M L M N E G L S G Y F E P N E G W M Y S N T G Y V L L A V I I E K A S G M S Y A D F I K T S I F L P A G M N E T R V Y N R R L S P E R I D H Y A Y G Y V Y D V H S E T Y V L P D E L E E T N Y V V Y L D G I Q G D G T V N S V T S D L F R F D Q A L Y Q D D F I S K A S K E S A F S P V R L N N G E T I D Y G F G W V L Q N S P E K G R I V S H S G G W P G Y S T M M I R Y I D H R K T L I Y L S N K E E D T E Y E Q A I L K A A E H I L F G Q P Y D V P E R P A D K K K K A I D T A I Y S R Y V G S Y L L Q D G T A A Q V T T E N E R L Y L E I A G Q L R L E L F P S S E T R F F L R A L S V E V E F T L G E D A A K S F I L Y E D G S E E E A V R T K
- Accession
- Protein length
Full Length
- Predicted molecular weight
67.4 kDa
- Amino acids
1 to 451
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Probably involved in peptidoglycan modification during cortex synthesis.
- Sequence similarities
Belongs to the beta-lactamase family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Penicillin-binding protein 4 (PBP4) also known as D-alanyl-D-alanine carboxypeptidase DacB plays an important role in bacterial cell wall synthesis by acting as a peptidase. Functionally PBP4 is a high-molecular-weight penicillin-binding protein that has an approximate mass of 55 kDa. It is expressed predominantly in the membrane of Gram-negative bacteria and it is involved in the late stages of peptidoglycan assembly where it catalyzes cross-linking of peptide chains. PBP4's activity ensures the structural integrity and regulation of bacterial cell walls.
- Biological function summary
PBP4 serves a critical function in bacterial growth and survival by participating in peptidoglycan remodeling. It functions as part of a larger enzymatic complex that adjusts and repairs the bacterial sacculus during cell division and sporulation. This is especially important in maintaining balance between peptidoglycan synthesis and degradation which influences cell shape and resistance to osmotic stress. PBP4's enzymatic activity is essential for bacterial cells to adapt to different environmental conditions by modifying their cell walls accordingly.
- Pathways
PBP4 interacts within the bacterial peptidoglycan biosynthesis pathway. It works alongside other penicillin-binding proteins like PBP1A and PBP1B to contribute to the sequential processing of peptidoglycan layers. These proteins coordinate to ensure the peptidoglycan structure is resistant yet flexible enough to allow cell growth and division. The modifications carried out by PBP4 and related proteins are integral to the broader cell wall biogenesis pathway impacting bacterial morphogenesis and physical resilience.
- Associated diseases and disorders
Penicillin-binding protein 4 is significant in the context of antibiotic resistance particularly in infections caused by resistant strains of bacteria such as Methicillin-resistant Staphylococcus aureus (MRSA). Mutations and overexpression of PBP4 can alter its affinity for antibiotics like penicillin leading to treatment failures. This target holds potential for new therapeutic interventions specifically by designing drugs that can effectively bind and inhibit these resistant enzymes. PBP2A is another protein typically associated with PBP4 in resistant strains where their altered functions impede the action of beta-lactam antibiotics complicating treatment strategies.
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SDS-PAGE - Recombinant B. subtilis Penicillin-binding protein 4 (His tag) (ab226290)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226290 with 5% enrichment gel and 15% separation gel.
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