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AB134845

Recombinant Bacteriophage P1 Cre recombinase protein

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(1 Publication)

Recombinant Bacteriophage P1 Cre recombinase protein is a Bacteriophage P1 Full Length protein, in the 2 to 343 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Recombinase cre, cre, Cre, Recombinase cre, Cyclization recombinase

2 Images
Functional Studies - Recombinant Bacteriophage P1 Cre recombinase protein (AB134845)
  • FuncS

Unknown

Functional Studies - Recombinant Bacteriophage P1 Cre recombinase protein (AB134845)

Analysis of 0.25 x106 293-LoxP-eGFP report cell line with 1 μg ab134845. Cells were cultivated overnight before viewing GFP positive cells.

Western blot - Recombinant Bacteriophage P1 Cre recombinase protein (AB134845)
  • WB

Supplier Data

Western blot - Recombinant Bacteriophage P1 Cre recombinase protein (AB134845)

All lanes:

Western blot - Anti-Cre recombinase antibody [EPR13835] (<a href='/en-us/products/primary-antibodies/cre-recombinase-antibody-epr13835-ab188568'>ab188568</a>) at 1/5000 dilution

Lane 1:

Active bacteriophage p1 Cre recombinase full length protein at 0.001 µg

Lane 1:

Western blot - Recombinant Bacteriophage P1 Cre recombinase protein (ab134845) at 0.001 µg

Lane 2:

E. coli at 10 µg

Lane 3:

Saccharomyces cerevisiae at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa,86 kDa

false

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

T7 tag N-Terminus His tag N-Terminus TEV tag N-Terminus 11R tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

No

Accession

P06956

Animal free

No

Carrier free

No

Species

Bacteriophage P1

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.08% Potassium chloride, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Primary Antibodies

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Anti-Cre recombinase antibody [EPR13835]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Applications:
<p>1. Protein intracellular transduction for in vitro Cre-loxP recombination.</p>
<p>2. Negative control for 11R-Tag human transcription factor mediated cell differentiation assay in vitro.</p>
<p>Functional Assay: Mix 1μg Cre-11R protein with 2ul ProFectinTM by following ProFectinTM manufacture suggested protocol, and incubate transfection mixture  0.25 x10<sup>6</sup> 293-LoxP-eGFP cell line / well (6 well plate) for 30 min in serum free medium, will achieve &gt; 35% transduction efficiency. (Positive for GFP cells).</p>
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Sequence info

[{"sequence":"MASMTGGQQMGRGHHHHHHGNLYFQGGEFPPKKKRKVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDGDLEESGGGGSPGRRRRRRRRRRR","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":343,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P06956","tags":[{"tag":"T7","terminus":"N-Terminus"},{"tag":"His","terminus":"N-Terminus"},{"tag":"TEV","terminus":"N-Terminus"},{"tag":"11R","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab134845 is chromatographically purified.

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General info

Function

Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.

Sequence similarities

Belongs to the 'phage' integrase family.

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Product protocols

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Target data

Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.
See full target information Recombinase cre
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Current protocols 1:e278 PubMed34807521

2021

Assembly of Long-Adapter Single-Strand Oligonucleotide (LASSO) Probes for Massively Parallel Capture of Kilobase Size DNA Targets.

Applications

Unspecified application

Species

Unspecified reactive species

Lamia Chkaiban,Lorenzo Tosi,Biju Parekkadan
View all publications
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Product promise

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