Recombinant Bovine Coronavirus Hemagglutinin esterase protein (His tag) is a Bovine coronavirus Mebus Full Length protein, expressed in Yeast, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Yeast
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. May become a target for both the humoral and the cellular branches of the immune system.
Targets
Hemagglutinin esterase
Alternative names
Hemagglutinin-esterase, HE protein, E3 glycoprotein, 2b, HE
Recommended products
Recombinant Bovine Coronavirus Hemagglutinin esterase protein (His tag) is a Bovine coronavirus Mebus Full Length protein, expressed in Yeast, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Yeast
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P15776-1
- Animal free
No
- Species
Bovine coronavirus Mebus
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- Accession
- Protein length
Full Length
- Predicted molecular weight
47.7 kDa
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. May become a target for both the humoral and the cellular branches of the immune system.
- Sequence similarities
Belongs to the influenza type C/coronaviruses hemagglutinin-esterase family.
- Post-translational modifications
N-glycosylated in the host RER.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Hemagglutinin esterase (HE) is a protein with both hemagglutinin and esterase activities often referred to as HE or HEF. It possesses a mass of approximately 65 kDa. This protein is expressed on the surface of certain viruses mainly within the Orthomyxoviridae and Coronaviridae families. Mechanically HE contributes to viral entry and release by facilitating the receptor-binding process and cleaving sialic acid residues from host cell glycoproteins enabling viral particles to disseminate efficiently.
- Biological function summary
HE plays an important role in the life cycle of enveloped viruses particularly within the viral entry mechanism. It is often not part of a larger complex but works alongside other viral proteins such as the spike protein in coronaviruses. Its dual enzymatic functions allow for both attachment to host cell receptors and the cleavage of sialic acids which are critical for efficient viral infection and spread. These actions ensure the virus can efficiently infect host cells and efficiently propagate.
- Pathways
HE involves itself in the sialic acid-mediated viral entry pathway in host cells. Its functional role is significant in this pathway ensuring successful replication and spread. HE works closely with other viral proteins such as neuraminidase involved in the release of progeny viruses. Together these proteins facilitate critical steps in the viral replication cycle contributing to the infectivity and transmission of the virus.
- Associated diseases and disorders
HE has notable implications in influenza as well as some coronavirus infections. It relates significantly to transmissible gastroenteritis virus (TGEV) in swine and other respiratory diseases in humans. The esterase activity of HE is mirrored in its relationship with proteins like neuraminidase with the ability to modify host-cell glycoconjugates influencing viral pathogenesis. This understanding of HE can lead to better therapeutic strategies targeting these infections specifically.
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1 product image
SDS-PAGE - Recombinant Bovine Coronavirus Hemagglutinin esterase protein (His tag) (ab226232)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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