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AB226232

Recombinant Bovine Coronavirus Hemagglutinin esterase protein (His tag)

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Recombinant Bovine Coronavirus Hemagglutinin esterase protein (His tag) is a Bovine coronavirus Mebus Full Length protein, expressed in Yeast, with >90%, suitable for SDS-PAGE.

View Alternative Names

2b, HE, Hemagglutinin-esterase, HE protein, E3 glycoprotein

1 Images
SDS-PAGE - Recombinant Bovine Coronavirus Hemagglutinin esterase protein (His tag) (AB226232)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Bovine Coronavirus Hemagglutinin esterase protein (His tag) (AB226232)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>90% SDS-PAGE

Expression system

Yeast

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P15776

Animal free

No

Carrier free

No

Species

Bovine coronavirus Mebus

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"47.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P15776","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hemagglutinin esterase (HE) is a protein with both hemagglutinin and esterase activities often referred to as HE or HEF. It possesses a mass of approximately 65 kDa. This protein is expressed on the surface of certain viruses mainly within the Orthomyxoviridae and Coronaviridae families. Mechanically HE contributes to viral entry and release by facilitating the receptor-binding process and cleaving sialic acid residues from host cell glycoproteins enabling viral particles to disseminate efficiently.
Biological function summary

HE plays an important role in the life cycle of enveloped viruses particularly within the viral entry mechanism. It is often not part of a larger complex but works alongside other viral proteins such as the spike protein in coronaviruses. Its dual enzymatic functions allow for both attachment to host cell receptors and the cleavage of sialic acids which are critical for efficient viral infection and spread. These actions ensure the virus can efficiently infect host cells and efficiently propagate.

Pathways

HE involves itself in the sialic acid-mediated viral entry pathway in host cells. Its functional role is significant in this pathway ensuring successful replication and spread. HE works closely with other viral proteins such as neuraminidase involved in the release of progeny viruses. Together these proteins facilitate critical steps in the viral replication cycle contributing to the infectivity and transmission of the virus.

HE has notable implications in influenza as well as some coronavirus infections. It relates significantly to transmissible gastroenteritis virus (TGEV) in swine and other respiratory diseases in humans. The esterase activity of HE is mirrored in its relationship with proteins like neuraminidase with the ability to modify host-cell glycoconjugates influencing viral pathogenesis. This understanding of HE can lead to better therapeutic strategies targeting these infections specifically.

Specifications

Form

Liquid

General info

Function

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. May become a target for both the humoral and the cellular branches of the immune system.

Sequence similarities

Belongs to the influenza type C/coronaviruses hemagglutinin-esterase family.

Post-translational modifications

N-glycosylated in the host RER.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. May become a target for both the humoral and the cellular branches of the immune system.
See full target information HE

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