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AB224852

Recombinant Brucella abortus Malate dehydrogenase protein (His tag)

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Recombinant Brucella abortus Malate dehydrogenase protein (His tag) is a Brucella abortus S19 Full Length protein, in the 1 to 320 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

BAbS19_I18080, mdh, Malate dehydrogenase

2 Images
Mass Spectrometry - Recombinant Brucella abortus Malate dehydrogenase protein (His tag) (AB224852)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Brucella abortus Malate dehydrogenase protein (His tag) (AB224852)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab224852 could indicate that this peptide derived from E.coli-expressed Brucella abortus (strain S19) mdh.

Mass Spectrometry - Recombinant Brucella abortus Malate dehydrogenase protein (His tag) (AB224852)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Brucella abortus Malate dehydrogenase protein (His tag) (AB224852)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab224852 could indicate that this peptide derived from E.coli-expressed Brucella abortus (strain S19) mdh.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

B2S881

Animal free

No

Carrier free

No

Species

Brucella abortus S19

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Sequence info

[{"sequence":"MARNKIALIGSGMIGGTLAHLAGLKELGDVVLFDIAEGTPQGKGLDIAESSPVDGFDAKFTGANDYAAIEGADVVIVTAGVPRKPGMSRDDLLGINLKVMEQVGAGIKKYAPEAFVICITNPLDAMVWALQKFSGLPAHKVVGMAGVLDSARFRYFLSEEFNVSVEDVTVFVLGGHGDSMVPLARYSTVAGIPLPDLVKMGWTSQDKLDKIIQRTRDGGAEIVGLLKTGSAFYAPAASAIQMAESYLKDKKRVLPVAAQLSGQYGVKDMYVGVPTVIGANGVERIIEIDLDKDEKAQFDKSVASVAGLCEACIGIAPSLK","proteinLength":"Full Length","predictedMolecularWeight":"53.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":320,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"B2S881","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Brucella abortus Malate dehydrogenase also known as MDH is an enzyme that plays a significant role in the citric acid cycle. This enzyme catalyzes the conversion of malate to oxaloacetate through the reduction of NAD+ to NADH. MDH has a molecular mass of approximately 32 kDa. Within the bacterial cells it is expressed in the cytoplasm where it participates in metabolic reactions critical for energy production.
Biological function summary

The enzyme facilitates the energy transformation processes necessary for Brucella abortus to survive and replicate in host cells. It is not known to form part of a larger enzyme complex but interacts with other metabolic enzymes to ensure proper function of the tricarboxylic acid (TCA) cycle. Efficient operation of MDH is essential for providing the intermediates and reducing equivalents needed for other biological processes.

Pathways

Malate dehydrogenase contributes to the functionality of the TCA cycle and the glyoxylate shunt. These pathways are important for the survival of Brucella abortus in nutrient-limited conditions such as inside host macrophages. Malate dehydrogenase works alongside other key proteins like citrate synthase and isocitrate lyase coordinating effectively to maintain cellular energy balance and metabolic flexibility.

Brucella abortus Malate dehydrogenase connects to brucellosis a zoonotic disease affecting various mammals including humans. The bacterium’s ability to proliferate within host cells partly depends on the activity of MDH and related proteins such as succinate dehydrogenase. Understanding this enzyme's role helps in devising therapeutic interventions aimed at inhibiting Brucella abortus' energy production thereby reducing infection rates and disease severity.

Specifications

Form

Liquid

General info

Function

Catalyzes the reversible oxidation of malate to oxaloacetate.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 3 family.

Product protocols

Target data

Catalyzes the reversible oxidation of malate to oxaloacetate.
See full target information mdh

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