Recombinant Brucella abortus Malate dehydrogenase protein (His tag) is a Brucella abortus S19 Full Length protein, in the 1 to 320 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M A R N K I A L I G S G M I G G T L A H L A G L K E L G D V V L F D I A E G T P Q G K G L D I A E S S P V D G F D A K F T G A N D Y A A I E G A D V V I V T A G V P R K P G M S R D D L L G I N L K V M E Q V G A G I K K Y A P E A F V I C I T N P L D A M V W A L Q K F S G L P A H K V V G M A G V L D S A R F R Y F L S E E F N V S V E D V T V F V L G G H G D S M V P L A R Y S T V A G I P L P D L V K M G W T S Q D K L D K I I Q R T R D G G A E I V G L L K T G S A F Y A P A A S A I Q M A E S Y L K D K K R V L P V A A Q L S G Q Y G V K D M Y V G V P T V I G A N G V E R I I E I D L D K D E K A Q F D K S V A S V A G L C E A C I G I A P S L K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the reversible oxidation of malate to oxaloacetate.
Alternative names
BAbS19_I18080, mdh, Malate dehydrogenase
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Recombinant Brucella abortus Malate dehydrogenase protein (His tag) is a Brucella abortus S19 Full Length protein, in the 1 to 320 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- B2S881-1
- Animal free
- No
- Species
- Brucella abortus S19
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M A R N K I A L I G S G M I G G T L A H L A G L K E L G D V V L F D I A E G T P Q G K G L D I A E S S P V D G F D A K F T G A N D Y A A I E G A D V V I V T A G V P R K P G M S R D D L L G I N L K V M E Q V G A G I K K Y A P E A F V I C I T N P L D A M V W A L Q K F S G L P A H K V V G M A G V L D S A R F R Y F L S E E F N V S V E D V T V F V L G G H G D S M V P L A R Y S T V A G I P L P D L V K M G W T S Q D K L D K I I Q R T R D G G A E I V G L L K T G S A F Y A P A A S A I Q M A E S Y L K D K K R V L P V A A Q L S G Q Y G V K D M Y V G V P T V I G A N G V E R I I E I D L D K D E K A Q F D K S V A S V A G L C E A C I G I A P S L K
- Accession
- B2S881
- Protein length
- Full Length
- Predicted molecular weight
- 53.7 kDa
- Amino acids
- 1 to 320
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Catalyzes the reversible oxidation of malate to oxaloacetate.
- Sequence similarities
Belongs to the LDH/MDH superfamily. MDH type 3 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Brucella abortus Malate dehydrogenase also known as MDH is an enzyme that plays a significant role in the citric acid cycle. This enzyme catalyzes the conversion of malate to oxaloacetate through the reduction of NAD+ to NADH. MDH has a molecular mass of approximately 32 kDa. Within the bacterial cells it is expressed in the cytoplasm where it participates in metabolic reactions critical for energy production.
- Biological function summary
The enzyme facilitates the energy transformation processes necessary for Brucella abortus to survive and replicate in host cells. It is not known to form part of a larger enzyme complex but interacts with other metabolic enzymes to ensure proper function of the tricarboxylic acid (TCA) cycle. Efficient operation of MDH is essential for providing the intermediates and reducing equivalents needed for other biological processes.
- Pathways
Malate dehydrogenase contributes to the functionality of the TCA cycle and the glyoxylate shunt. These pathways are important for the survival of Brucella abortus in nutrient-limited conditions such as inside host macrophages. Malate dehydrogenase works alongside other key proteins like citrate synthase and isocitrate lyase coordinating effectively to maintain cellular energy balance and metabolic flexibility.
- Associated diseases and disorders
Brucella abortus Malate dehydrogenase connects to brucellosis a zoonotic disease affecting various mammals including humans. The bacterium’s ability to proliferate within host cells partly depends on the activity of MDH and related proteins such as succinate dehydrogenase. Understanding this enzyme's role helps in devising therapeutic interventions aimed at inhibiting Brucella abortus' energy production thereby reducing infection rates and disease severity.
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2 product images
Mass Spectrometry - Recombinant Brucella abortus Malate dehydrogenase protein (His tag) (ab224852)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab224852 could indicate that this peptide derived from E.coli-expressed Brucella abortus (strain S19) mdh.
Mass Spectrometry - Recombinant Brucella abortus Malate dehydrogenase protein (His tag) (ab224852)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab224852 could indicate that this peptide derived from E.coli-expressed Brucella abortus (strain S19) mdh.
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