Recombinant C4 Protease protein is a Tobacco vein mottling virus Full Length protein, in the 2002 to 2236 aa range, expressed in Tobacco Vein Mottling Virus, with >95% purity and suitable for SDS-PAGE, FuncS.
M A S M T G G Q Q M G R G H H H H H H G N L Y F Q G G E F S K A L L K G V R D F N P I S A C V W L L E N S S D G H S E R L F G I G F G P Y I I A N Q H L F R R N N G E L T I K T M H G E F K V K N S T Q L Q M K P V E G R D I I V I K M A K D F P P F P Q K L K F R Q P T I K D R V C M V S T N F Q Q K S V S S L V S E S S H I V H K E D T S F W Q H W I T T K D G Q C G S P L V S I I D G N I L G I H S L T H T T N G S N Y F V E F P E K F V A T Y L D A A D G W C K N W K F N A D K I S W G S F T L V E D A P E D D F M A K K T V A A I M D
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes The "standard" reaction buffer for TVMV C4 protease is 50 mM Tris-HCl (pH 8.0), 0.5mM EDTA and 1mM DTT. The duration of the cleavage reaction is typically overnight,although lots of cleavage will happen in the first few hours and prolonged incubation times maynot lead to proportional increases in cleavage. TVMV C4 protease is maximally active at 34 °C,but we recommend performing the digest at room temperature (20 °C) or 4 °C overnight. |
Helper component proteinase. Required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity. Cytoplasmic inclusion protein. Has helicase activity. It may be involved in replication. 6 kDa protein 1. Indispensable for virus replication (By similarity). Reduces the abundance of host transcripts related to jasmonic acid biosynthesis therefore altering the host defenses (By similarity). In order to increase its own stability, decreases host protein degradation pathways (By similarity). 6 kDa protein 2. Indispensable for virus replication. Viral genome-linked protein. Mediates the cap-independent, EIF4E-dependent translation of viral genomic RNAs (By similarity). Binds to the cap-binding site of host EIF4E and thus interferes with the host EIF4E-dependent mRNA export and translation (By similarity). VPg-RNA directly binds EIF4E and is a template for transcription (By similarity). Also forms trimeric complexes with EIF4E-EIF4G, which are templates for translation (By similarity). Nuclear inclusion protein A. Has RNA-binding and proteolytic activities (By similarity). Main protease that processes most of the polyprotein cleavages (PubMed:20862670). Nuclear inclusion protein B. An RNA-dependent RNA polymerase that plays an essential role in the virus replication. Capsid protein. Involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification.
C4 Protease
Genome polyprotein
Recombinant C4 Protease protein is a Tobacco vein mottling virus Full Length protein, in the 2002 to 2236 aa range, expressed in Tobacco Vein Mottling Virus, with >95% purity and suitable for SDS-PAGE, FuncS.
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Helper component proteinase
Belongs to the potyviridae genome polyprotein family.
Viral genome-linked protein
This product is an active protein and may elicit a biological response in vivo, handle with caution.
C4 Protease could be easily removed from the cleavage reaction by affinity chromatography using the polyhistidine tag at N-terminal.
Complete digestion 50ug of MBP-IL6 fusion protein using 1 ug TVMV C4 Protease (in 1:50 ratio) for 12 hours at 4C.
C4 Protease also known as C4 P is a proteolytic enzyme with a molecular mass of around 80 kDa. It cleaves specific peptide bonds in substrates facilitating protein degradation and turnover. You can find it expressed in various tissues including the liver and immune cells. Its activity is regulated by factors like pH and the presence of inhibitors making it selectively active under certain physiological conditions.
C4 Protease serves multiple roles in immune system processes. It participates as an important component of the complement system a critical part of innate immunity. It cleaves C4 into C4a and C4b during the activation of the complement pathway aiding in pathogen opsonization and cell lysis. The enzyme functions are essential for maintaining immune surveillance and modulating inflammation.
This target plays important roles in the complement cascade and coagulation pathways. In the complement pathway C4 Protease activates components like C2 facilitating opsonization and membrane attack complex formation. Its activity also interlinks with proteins such as complement component C3 which further propagates the immune response cascade. By acting within these pathways C4 Protease helps balance immune defense and homeostasis.
C4 Protease shows associations with autoimmune conditions like systemic lupus erythematosus and hereditary angioedema. In these conditions dysregulation of its activity can cause excessive complement activation or deficiency in regulatory components. Related proteins like complement component C1 are also involved with abnormalities in these proteins contributing to disease pathology. Understanding these connections advances research into potential therapeutic interventions for these immune-related disorders.
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