Recombinant Chinese hamster P4HB protein (His tag) is a Chinese hamster Full Length protein, in the 20 to 509 aa range, expressed in Yeast, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Yeast
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
D A P E E E D N V L V L K K S N F A E A L A A H N Y L L V E F Y A P W C G H C K A L A P E Y A K A A A K L K A E G S E I R L A K V D A T E E S D L A Q Q Y G V R G Y P T I K F F K N G D T A S P K E Y T A G R E A D D I V N W L K K R T G P A A T T L S D T A A A E T L I D S S E V A V I G F F K D V E S D S A K Q F L L A A E A V D D I P F G I T S N S G V F S K Y Q L D K D G V V L F K K F D E G R N N F E G E V T K E K L L D F I K H N Q L P L V I E F T E Q T A P K I F G G E I K T H I L L F L P K S V S D Y D G K L G N F K K A A E G F K G K I L F I F I D S D H T D N Q R I L E F F G L K K E E C P A V R L I T L E E E M T K Y K P E S D E L T A E K I T E F C H R F L E G K I K P H L M S Q E L P E D W D K Q P V K V L V G K N F E E V A F D E K K N V F V E F Y A P W C G H C K Q L A P I W D K L G E T Y K D H E N I I I A K M D S T A N E V E A V K V H S F P T L K F F P A T A D R T V I D Y N G E R T L D G F K K F L E S G G Q D G A G D D D D V D L E E A L E P D M E E D D D Q K A V K D E L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations and following phosphorylation by FAM20C, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts as a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.
Alternative names
PDIA1, P4HB, Protein disulfide-isomerase, PDI, Prolyl 4-hydroxylase subunit beta, p58
Recommended products
Recombinant Chinese hamster P4HB protein (His tag) is a Chinese hamster Full Length protein, in the 20 to 509 aa range, expressed in Yeast, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Yeast
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q8R4U2-1
- Animal free
- No
- Species
- Chinese hamster
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- D A P E E E D N V L V L K K S N F A E A L A A H N Y L L V E F Y A P W C G H C K A L A P E Y A K A A A K L K A E G S E I R L A K V D A T E E S D L A Q Q Y G V R G Y P T I K F F K N G D T A S P K E Y T A G R E A D D I V N W L K K R T G P A A T T L S D T A A A E T L I D S S E V A V I G F F K D V E S D S A K Q F L L A A E A V D D I P F G I T S N S G V F S K Y Q L D K D G V V L F K K F D E G R N N F E G E V T K E K L L D F I K H N Q L P L V I E F T E Q T A P K I F G G E I K T H I L L F L P K S V S D Y D G K L G N F K K A A E G F K G K I L F I F I D S D H T D N Q R I L E F F G L K K E E C P A V R L I T L E E E M T K Y K P E S D E L T A E K I T E F C H R F L E G K I K P H L M S Q E L P E D W D K Q P V K V L V G K N F E E V A F D E K K N V F V E F Y A P W C G H C K Q L A P I W D K L G E T Y K D H E N I I I A K M D S T A N E V E A V K V H S F P T L K F F P A T A D R T V I D Y N G E R T L D G F K K F L E S G G Q D G A G D D D D V D L E E A L E P D M E E D D D Q K A V K D E L
- Accession
- Q8R4U2
- Protein length
- Full Length
- Predicted molecular weight
- 56.9 kDa
- Amino acids
- 20 to 509
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations and following phosphorylation by FAM20C, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts as a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.
- Sequence similarities
Belongs to the protein disulfide isomerase family.
- Post-translational modifications
Phosphorylation of Ser-359 by FAM20C is induced by endoplasmic reticulum stress and results in a functional switch from oxidoreductase to molecular chaperone. It also promotes interaction with ERN1.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
P4HB is a protein known as Protein Disulfide Isomerase (PDI). It plays a significant role in catalyzing the formation of disulfide bonds. This protein helps to fold newly synthesized proteins by shuffling disulfide bonds. It has a molecular mass of around 57 kDa. P4HB is mainly expressed in the endoplasmic reticulum of cells. While its alternative name is ERp59 P4HB is integral to the protein folding machinery inside cells.
- Biological function summary
P4HB functions in protein folding and assembly. By acting as a chaperone it protects proteins from misfolding and aggregation. P4HB operates as part of a larger multi-protein complex that assists in maintaining protein structure under stress conditions in the cell. Its activity ensures protein stability and proper cellular function important for cell viability and health.
- Pathways
P4HB plays a central role in the unfolded protein response (UPR) and oxidative protein folding pathway. It interacts with proteins such as calnexin and calreticulin through its involvement in these pathways. During oxidative protein folding P4HB introduces disulfide bonds into nascent proteins while removing incorrect ones ensuring efficient protein quality control.
- Associated diseases and disorders
P4HB alteration has connections to diseases like cancer and neurodegenerative disorders. Its overexpression and activity can promote tumor growth by aiding cancerous cell survival through protein homeostasis. Moreover P4HB's association with amyloid precursor protein impacts the progression of Alzheimer's disease. These connections make P4HB a relevant target for research into therapeutic interventions.
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1 product image
SDS-PAGE - Recombinant Chinese hamster P4HB protein (His tag) (ab241446)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab241446.
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