Recombinant Chlamydia trachomatis OmcB protein (His tag) is a Chlamydia trachomatis D/UW-3/CX Fragment protein, in the 41 to 196 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
L A D T K A K D N T S H K S K K A R K N H S K E T P V D R K E V A P V H E S K A T G P K Q D S C F G R M Y T V K V N D D R N V E I T Q A V P E Y A T V G S P Y P I E I T A T G K R D C V D V I I T Q Q L P C E A E F V R S D P A T T P T A D G K L V W K I D R L G Q G E K S K I T V W V K P L K E G C C F T A A T V C A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
In elementary bodies (EBs, the infectious stage, which is able to survive outside the host cell) provides the structural integrity of the outer envelope through disulfide cross-links with the small cysteine-rich protein and the major outer membrane protein. It has been described in publications as the Sarkosyl-insoluble COMC (Chlamydia outer membrane complex), and serves as the functional equivalent of peptidoglycan (By similarity).
Alternative names
omp2, omp2B, CT_443, omcB, Large cysteine-rich periplasmic protein OmcB, Large-CRP, 60 kDa cysteine-rich OMP, 60 kDa outer membrane protein, Cysteine-rich outer membrane protein, CRP
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Recombinant Chlamydia trachomatis OmcB protein (His tag) is a Chlamydia trachomatis D/UW-3/CX Fragment protein, in the 41 to 196 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P0CC04-1
- Animal free
- No
- Species
- Chlamydia trachomatis D/UW-3/CX
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- L A D T K A K D N T S H K S K K A R K N H S K E T P V D R K E V A P V H E S K A T G P K Q D S C F G R M Y T V K V N D D R N V E I T Q A V P E Y A T V G S P Y P I E I T A T G K R D C V D V I I T Q Q L P C E A E F V R S D P A T T P T A D G K L V W K I D R L G Q G E K S K I T V W V K P L K E G C C F T A A T V C A
- Accession
- P0CC04
- Protein length
- Fragment
- Predicted molecular weight
- 33.1 kDa
- Amino acids
- 41 to 196
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
In elementary bodies (EBs, the infectious stage, which is able to survive outside the host cell) provides the structural integrity of the outer envelope through disulfide cross-links with the small cysteine-rich protein and the major outer membrane protein. It has been described in publications as the Sarkosyl-insoluble COMC (Chlamydia outer membrane complex), and serves as the functional equivalent of peptidoglycan (By similarity).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
OmcB is a significant protein involved in electron transport processes also known as outer membrane cytochrome B. This protein plays an essential role in the transfer of electrons across the outer membrane in bacteria such as Geobacter sulfurreducens. OmcB is a large protein with an estimated molecular weight of approximately 100 kDa. It is expressed in the outer membrane of the bacterium facilitating its role in electron transfer during the bacterium's anaerobic respiration.
- Biological function summary
Its function enables the transfer of electrons to extracellular electron acceptors such as iron and manganese oxides which are important for the metabolism of Geobacter species. OmcB typically associates with other cytochromes to form a multi-component electron transport complex. This complex plays an important role in facilitating the reduction of metal ions outside the cell therefore sustaining the bacterium's ability to survive in iron-rich environments.
- Pathways
OmcB participates in the bacterial electron transport chain pathways which are vital for energy metabolism. In these pathways OmcB functions alongside other proteins such as OmcZ and other cytochromes to efficiently transfer electrons and generate energy. This process supports the bacterium's growth and energy conversion efficiency by connecting metabolic pathways to electron acceptors located outside the cell.
- Associated diseases and disorders
OmcB contributes to the biodegradation of environmental pollutants through electron transfer to metal oxides. While it is not directly implicated in human diseases the protein plays a role in environmental processes that indirectly affect human health. OmcB's function links it to studies focused on bioremediation strategies where proteins such as MtrC from other bacterial species demonstrate similar roles in reducing environmental contaminants.
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1 product image
SDS-PAGE - Recombinant Chlamydia trachomatis OmcB protein (His tag) (ab224862)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab224862 with 5% enrichment gel and 15% separation gel.
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