Recombinant Chlamydomonas reinhardtii Autolysin protein (His tag)
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Recombinant Chlamydomonas reinhardtii Autolysin protein (His tag) is a Chlamydomonas reinhardtii Full Length protein, in the 184 to 638 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.
View Alternative Names
Autolysin, Gamete lytic enzyme, Gametolysin, GLE
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Autolysins play a vital role in bacterial cell wall turnover enabling processes such as cell division growth and separation after division. During these processes they often work alone or as part of multi-protein complexes which finely regulate their activity to ensure controlled degradation of the cell wall. Maintaining balance between their activity and inhibitors helps bacteria control cell shape and integrity.
Pathways
Autolysins integrate into pathways involved in bacterial morphogenesis and antibiotic resistance. They interact with proteins such as penicillin-binding proteins in the penicillin resistance pathway and coordinate with enzymes involved in peptidoglycan synthesis and remodeling. The balance between synthesis and degradation activities is critical for bacteria to withstand environmental stressors and external antibiotic pressures.
Specifications
Form
Liquid
General info
Function
Mediates digestion of the cell walls of the 2 mating type gametes during mating as a necessary prelude to cell fusion. This enzyme acts specifically on the framework proteins (inner wall) of the cell wall, cleaving several model peptides at specific sites.
Sequence similarities
Belongs to the peptidase M11 family.
Post-translational modifications
Present in 2 forms: an inactive V-form in vegetative cells and an active and soluble G-form. The V-form enzyme may be converted to the G-form enzyme during gametic differentiation under nitrogen-starved conditions.
Target data
Product promise
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