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AB226279

Recombinant Chlamydomonas reinhardtii Autolysin protein (His tag)

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Recombinant Chlamydomonas reinhardtii Autolysin protein (His tag) is a Chlamydomonas reinhardtii Full Length protein, in the 184 to 638 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

Autolysin, Gamete lytic enzyme, Gametolysin, GLE

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P31178

Animal free

No

Carrier free

No

Species

Chlamydomonas reinhardtii

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Sequence info

[{"sequence":"EIYAGKPIDLRTIVYIMDFSSCKLSGWSAPATLTPEKVTSDMLRGASAPTNNLANYYGACSYEKTLFNPDNFLVLGPVPVPCIGGVTPPPRPPRPPRPPPRAGSTISSLSRRNDTYDDWWDLSKYCTASEQQAWERAAEAYAQAIVAQDPNSATGKKLQGILQWRERRRNIYILPPGVKCSWSGYADVTCTSATCSAYVRGYSDTNAMQVIMHEAMHNYGLEHAGRGTLEYGDATDVMGDFNKAGKGLLCPNAPNMYRIGWAKPINEPGVAPFQNATGAWGNLTAANFTTDPWIRGLVIPAQGTRDDNMIVVNVGAQSTRDGAMKATGAQAYYFSYRIKNTTAGGYDSGLTLDFHKKVLVHAYNGIQSERVFGFKSNLLDWGPNFQSRSNTWTSPFLAYNNGLGGGVRLVVQSTSDTQAVVDICRISENGKELSCDDGIDNDCDGLQDNEDPDCQ","proteinLength":"Full Length","predictedMolecularWeight":"65.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":638,"aminoAcidStart":184,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P31178","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Autolysin sometimes known as peptidoglycan hydrolase functions mechanically to break down peptidoglycan a major component of bacterial cell walls. This enzyme group includes proteins that vary significantly in mass. These enzymes are often found in the cell walls of Gram-positive bacteria such as Staphylococcus aureus and Bacillus subtilis. By hydrolyzing the glycosidic bonds in the peptidoglycan matrix autolysins facilitate important structural modifications and growth processes.
Biological function summary

Autolysins play a vital role in bacterial cell wall turnover enabling processes such as cell division growth and separation after division. During these processes they often work alone or as part of multi-protein complexes which finely regulate their activity to ensure controlled degradation of the cell wall. Maintaining balance between their activity and inhibitors helps bacteria control cell shape and integrity.

Pathways

Autolysins integrate into pathways involved in bacterial morphogenesis and antibiotic resistance. They interact with proteins such as penicillin-binding proteins in the penicillin resistance pathway and coordinate with enzymes involved in peptidoglycan synthesis and remodeling. The balance between synthesis and degradation activities is critical for bacteria to withstand environmental stressors and external antibiotic pressures.

Autolysins are connected to bacterial pathogenicity and resistance to antibiotics like methicillin-resistant Staphylococcus aureus (MRSA) infections. The dysregulation of autolysin activity contributes to bacterial invasiveness and the evasion of host immune responses. Proteins such as lysostaphin and autolysin regulators become essential targets in developing antibacterial therapies seeking to disrupt virulence mechanisms linked to autolysins.

Specifications

Form

Liquid

General info

Function

Mediates digestion of the cell walls of the 2 mating type gametes during mating as a necessary prelude to cell fusion. This enzyme acts specifically on the framework proteins (inner wall) of the cell wall, cleaving several model peptides at specific sites.

Sequence similarities

Belongs to the peptidase M11 family.

Post-translational modifications

Present in 2 forms: an inactive V-form in vegetative cells and an active and soluble G-form. The V-form enzyme may be converted to the G-form enzyme during gametic differentiation under nitrogen-starved conditions.

Product protocols

Target data

Mediates digestion of the cell walls of the 2 mating type gametes during mating as a necessary prelude to cell fusion. This enzyme acts specifically on the framework proteins (inner wall) of the cell wall, cleaving several model peptides at specific sites.
See full target information Autolysin

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