Recombinant Cynolmogus Monkey Apolipoprotein A-I/APOA1 Protein (His-tag) is a Cynomolgus monkey Full Length protein, expressed in Escherichia coli, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS, SDS-PAGE.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- MS, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
Amino acid sequence
D E P P Q T P W D R V K D L V T V Y V E A L K D S G K D Y V S Q F E G S A L G K Q L N L K L L D N W D S V T S T V S K L R E Q L G P V T Q E F W D N L E K E T E G L R Q E M S K D L E E V K A K V Q P Y L D D F Q K K W Q E E M E L Y R Q K V E P L R A E L H E G T R Q K L H E L H E K L S P L G E E V R D R A R A H V D A L R T H L A P Y S D E L R Q R L A A R L E A L K E N G G A R L A E Y H A K A S E H L S T L S E K A K P A L E D L R Q G L L P V L E S F K V S F L S A L E E Y T K K L S T Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Alternative names
Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1
Recommended products
Recombinant Cynolmogus Monkey Apolipoprotein A-I/APOA1 Protein (His-tag) is a Cynomolgus monkey Full Length protein, expressed in Escherichia coli, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS, SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- MS, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
- Accession
- P68292-1
- Animal free
- Yes
- Species
- Cynomolgus monkey
- Reconstitution
- Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10% PBS, 5% Trehalose
Sequence info
Amino acid sequence
- D E P P Q T P W D R V K D L V T V Y V E A L K D S G K D Y V S Q F E G S A L G K Q L N L K L L D N W D S V T S T V S K L R E Q L G P V T Q E F W D N L E K E T E G L R Q E M S K D L E E V K A K V Q P Y L D D F Q K K W Q E E M E L Y R Q K V E P L R A E L H E G T R Q K L H E L H E K L S P L G E E V R D R A R A H V D A L R T H L A P Y S D E L R Q R L A A R L E A L K E N G G A R L A E Y H A K A S E H L S T L S E K A K P A L E D L R Q G L L P V L E S F K V S F L S A L E E Y T K K L S T Q
- Accession
- P68292
- Protein length
- Full Length
- Predicted molecular weight
- 29.69 kDa
- Actual molecular weight
- 29.56 kDa
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
- Sequence similarities
Belongs to the apolipoprotein A1/A4/E family.
- Post-translational modifications
Glycosylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Apolipoprotein A-I (ApoA-I) is a major component of high-density lipoprotein (HDL) particles often referred to as 'good cholesterol'. It has a molecular mass of approximately 28 kDa. ApoA-I is mainly expressed in the liver and intestine. It plays a mechanical role in the reverse transport of cholesterol acting to facilitate the efflux of cholesterol from tissues to the liver for excretion. Its alternative names include ApoAI ApoA1 and a component of the AI kits used for measuring this protein.
- Biological function summary
ApoA-I functions in cholesterol homeostasis and inflammation. It is a structural component of the HDL complex that mobilizes cholesterol. ApoA-I acts as an activator of the enzyme lecithin-cholesterol acyltransferase (LCAT) which is essential for the maturation of HDL particles. This maturation is necessary for effective cholesterol transport and clearance. ApoA-I's ability to stabilize HDL particles and enhance their functionality makes it significant for maintaining lipid balance and cellular homeostasis.
- Pathways
The interaction of ApoA-I with HDL formation and function marks its role in lipid metabolism pathways. Its participation in the reverse cholesterol transport pathway highlights its influence on cardiovascular health. ApoA-I also interacts with other proteins like ApoA-II and paraoxonase-1 which further influence lipid metabolism and antioxidant activities. Understanding these relationships helps elucidate the dynamics of cholesterol removal from the bloodstream.
- Associated diseases and disorders
Disturbances in ApoA-I levels correlate with cardiovascular disease and atherosclerosis. Deficiency or dysfunction in ApoA-I can impair HDL function leading to poor cholesterol removal and buildup within arteries. It is also connected to amyloidosis where misfolded ApoA-I forms deposits in tissues. Understanding these pathological conditions helps researchers target ApoA-I in therapeutic strategies to mitigate disease progression often studying it alongside proteins like ApoB which is associated with low-density lipoprotein (LDL) particles.
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1 product image
SDS-PAGE - Recombinant Cynolmogus Monkey Apolipoprotein A-I/APOA1 Protein (His-tag) (ab322310)
SDS-PAGE analysis of ab322310 under reducing conditions for 2ug protein.
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