Recombinant Cynomolgus monkey CD80 protein (His tag) is a Fragment protein, in the 35 to 242 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, HPLC
- Biologically active
- No
Amino acid sequence
V I H V T K E V K E V A T L S C G H N V S V E E L A Q T R I Y W Q K E K K M V L T M M S G D M N I W P E Y K N R T I F D I T N N L S I V I L A L R P S D E G T Y E C V V L K Y E K D A F K R E H L A E V M L S V K A D F P T P S I T D F E I P P S N I R R I I C S T S G G F P E P H L S W L E N G E E L N A I S T T V S Q D P E T E L Y T V S S K L D F N M T T N H S F M C L I K Y G H L R V N Q T F N W N T P K Q E H F P D N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Alternative names
Activation B7-1 antigen, B lymphocyte activation antigen B7, B7, B7-1, B7-1 antigen, B7.1, BB1, CD28 antigen ligand 1, CD28LG, CD28LG1, CD80 antigen, CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), CD80 molecule, CD80_HUMAN, CTLA-4 counter-receptor B7.1, Costimulatory factor CD80, LAB7, T-lymphocyte activation antigen CD80, costimulatory molecule variant IgV-CD80
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Recombinant Cynomolgus monkey CD80 protein (His tag) is a Fragment protein, in the 35 to 242 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, HPLC
- Biologically active
- No
- Animal free
- No
- Reconstitution
- Reconstitute at 200 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
Sequence info
Amino acid sequence
- V I H V T K E V K E V A T L S C G H N V S V E E L A Q T R I Y W Q K E K K M V L T M M S G D M N I W P E Y K N R T I F D I T N N L S I V I L A L R P S D E G T Y E C V V L K Y E K D A F K R E H L A E V M L S V K A D F P T P S I T D F E I P P S N I R R I I C S T S G G F P E P H L S W L E N G E E L N A I S T T V S Q D P E T E L Y T V S S K L D F N M T T N H S F M C L I K Y G H L R V N Q T F N W N T P K Q E H F P D N
- Protein length
- Fragment
- Predicted molecular weight
- 25.8 kDa
- Amino acids
- 35 to 242
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
>90% by HPLC.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD80 also known as B7-1 is a cell surface protein with a molecular weight of approximately 60 kDa. It is expressed on antigen-presenting cells such as dendritic cells macrophages and B cells. The CD80 protein plays a mechanical role in immune response modulation by providing necessary signals for T cell activation and survival. CD80 interacts with receptors CD28 and CTLA-4 on T cells serving as a costimulatory molecule important for productive immune responses.
- Biological function summary
CD80 plays a significant role in the immune system's ability to distinguish between self and non-self. It belongs to the B7 family and participates in the formation of immunological synapses between T cells and antigen-presenting cells. This interaction is essential for triggering T cell proliferation and cytokine production forming a part of the adaptive immune response. CD80's expression increases in response to pathogen recognition enhancing the immune system's efficiency.
- Pathways
CD80 is integral to the regulation of T cell-mediated immune responses. It is involved in the CD28 co-stimulatory pathway which is vital for T cell activation and function. In this pathway CD80 interacts with proteins like CD28 and CTLA-4 to modulate immune activation and regulate immune tolerance. The proper function of CD80 within this pathway ensures a balanced immune reaction preventing autoimmunity while allowing effective defense against pathogens.
- Associated diseases and disorders
CD80 plays a role in autoimmune conditions and transplantation rejection. Its overexpression or dysregulation can contribute to diseases such as rheumatoid arthritis where it modifies T cell response and inflammation. Additionally CD80 is implicated in organ transplantation influencing graft rejection outcomes due to its role in modulating immune activation. Understanding CD80 interactions with CTLA-4 and CD28 offers insight into these conditions providing potential targets for therapeutic intervention.
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1 product image
SDS-PAGE - Recombinant Cynomolgus monkey CD80 protein (His tag) (ab220544)
SDS-PAGE analysis of reduced ab220544 stained overnight with Coomassie Blue.
The protein migrates as 43-55 kDa under reducing conditions (SDS-PAGE) due to different glycosylation.
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