Recombinant cynomolgus monkey PD-L1 protein (Active) is a Fragment protein, in the 19 to 238 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for FuncS, SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- FuncS, SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
F T V T V P K D L Y V V E Y G S N M T I E C K F P V E K Q L D L T S L I V Y W E M E D K N I I Q F V H G E E D L K V Q H S N Y R Q R A Q L L K D Q L S L G N A A L R I T D V K L Q D A G V Y R C M I S Y G G A D Y K R I T V K V N A P Y N K I N Q R I L V V D P V T S E H E L T C Q A E G Y P K A E V I W T S S D H Q V L S G K T T T T N S K R E E K L L N V T S T L R I N T T A N E I F Y C I F R R L D P E E N H T A E L V I P E L P L A L P P N E R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes The protein migrates as 37-42 kDa under reducing condition due to glycosylation. |
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Recombinant cynomolgus monkey PD-L1 protein (Active) is a Fragment protein, in the 19 to 238 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for FuncS, SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- FuncS, SDS-PAGE
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. Immobilized ab221343 at 1 μg/mL (100 μL/well) can bind Anti-Human PD-L1 MAb with a linear range of 0.4-3 ng/mL.
- Animal free
- No
- Reconstitution
- Reconstitute at 400 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
Sequence info
Amino acid sequence
- F T V T V P K D L Y V V E Y G S N M T I E C K F P V E K Q L D L T S L I V Y W E M E D K N I I Q F V H G E E D L K V Q H S N Y R Q R A Q L L K D Q L S L G N A A L R I T D V K L Q D A G V Y R C M I S Y G G A D Y K R I T V K V N A P Y N K I N Q R I L V V D P V T S E H E L T C Q A E G Y P K A E V I W T S S D H Q V L S G K T T T T N S K R E E K L L N V T S T L R I N T T A N E I F Y C I F R R L D P E E N H T A E L V I P E L P L A L P P N E R
- Protein length
- Fragment
- Predicted molecular weight
- 27.1 kDa
- Amino acids
- 19 to 238
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PD-L1 also known as Programmed Death-Ligand 1 or CD274 is a protein involved in immune modulation. Mechanically PD-L1 interacts with its receptors particularly PD-1 to regulate cellular immune responses. This transmembrane protein has a calculated molecular weight of approximately 33 kDa. PD-L1 is expressed on various cell types including tumor cells and immune cells such as dendritic cells macrophages and B cells. Its expression is often upregulated in response to inflammatory cytokines.
- Biological function summary
PD-L1 plays a central role in immune evasion mechanisms utilized by tumors. It is not part of a larger protein complex but directly interacts with PD-1 and CD80. When PD-L1 binds to PD-1 it sends inhibitory signals leading to decreased T cell activation and proliferation allowing cancer cells to avoid immune destruction. PD-L1 expression provides a mechanism for tumors to suppress immune surveillance facilitating tumor progression.
- Pathways
PD-L1 is integral to the immune checkpoint pathway which is an important regulator of immune response. The interaction between PD-L1 and PD-1 provides a mechanism for immune tolerance which is part of the broader adaptive immune system pathway. PD-L1 is related to other immune checkpoint proteins such as CTLA-4 in its function to limit autoreactivity and promote immune homeostasis.
- Associated diseases and disorders
PD-L1 is most associated with cancer particularly in tumors such as melanoma and non-small cell lung cancer. PD-L1 expression on tumor cells often correlates with poor prognosis. PD-L1 directly interacts with PD-1 in these cancers a target for immunotherapies such as checkpoint inhibitors which aim to block this interaction and restore immune activity against tumors. PD-L1 involvement extends to autoimmune disorders where altered expression can impact tolerance and lead to immune-related tissue damage.
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4 product images
SDS-PAGE - Recombinant cynomolgus monkey PD-L1 protein (Active) (ab221343)
Cynomolgus / Rhesus macaque PD-L1, His Tag (ab221343) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Functional Studies - Recombinant cynomolgus monkey PD-L1 protein (Active) (ab221343)
Immobilized ab221343 at 1 μg/mL (100 μL/well) can bind Anti-Human PD-L1 MAb with a linear range of 0.4-3 ng/mL.
Functional Studies - Recombinant cynomolgus monkey PD-L1 protein (Active) (ab221343)
Loaded Anti-Human PD-L1 MAb (Human IgG1) on AHC Biosensor, can bind ab221343 with an affinity constant of 19.1 nM as determined in BLI assay (ForteBio Octet Red96e).
Functional Studies - Recombinant cynomolgus monkey PD-L1 protein (Active) (ab221343)
Anti-Human PD-L1 MAb captured on CM5 chip via anti-human IgG Fc antibody surface can bind ab221343 with an affinity constant of 23.3 nM as determined in a SPR assay (Biacore T200).
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