Recombinant Cynomolgus monkey PD-L1 protein (Tagged) (Biotin) is a Fragment protein, in the 25 to 167 aa range, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Conjugation
- Biotin
Amino acid sequence
K D L Y V V E Y G S N M T I E C K F P V E K Q L D L T S L I V Y W E M E D K N I I Q F V H G E E D L K V Q H S N Y R Q R A Q L L K D Q L S L G N A A L R I T D V K L Q D A G V Y R C M I S Y G G A D Y K R I T V K V N A P Y N K I N Q R I L V V D P V T S E H E L T C Q A E G Y P K A E V I W
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Recommended products
Recombinant Cynomolgus monkey PD-L1 protein (Tagged) (Biotin) is a Fragment protein, in the 25 to 167 aa range, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Conjugation
- Biotin
- Animal free
- No
- Concentration
- Storage buffer
pH: 7.4
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- K D L Y V V E Y G S N M T I E C K F P V E K Q L D L T S L I V Y W E M E D K N I I Q F V H G E E D L K V Q H S N Y R Q R A Q L L K D Q L S L G N A A L R I T D V K L Q D A G V Y R C M I S Y G G A D Y K R I T V K V N A P Y N K I N Q R I L V V D P V T S E H E L T C Q A E G Y P K A E V I W
- Protein length
- Fragment
- Predicted molecular weight
- 75 kDa
- Amino acids
- 25 to 167
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Liquid
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Enzymatically biotin-labeled using Avitag™ technology.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PD-L1 also known as Programmed Death-Ligand 1 or CD274 is a protein involved in immune modulation. Mechanically PD-L1 interacts with its receptors particularly PD-1 to regulate cellular immune responses. This transmembrane protein has a calculated molecular weight of approximately 33 kDa. PD-L1 is expressed on various cell types including tumor cells and immune cells such as dendritic cells macrophages and B cells. Its expression is often upregulated in response to inflammatory cytokines.
- Biological function summary
PD-L1 plays a central role in immune evasion mechanisms utilized by tumors. It is not part of a larger protein complex but directly interacts with PD-1 and CD80. When PD-L1 binds to PD-1 it sends inhibitory signals leading to decreased T cell activation and proliferation allowing cancer cells to avoid immune destruction. PD-L1 expression provides a mechanism for tumors to suppress immune surveillance facilitating tumor progression.
- Pathways
PD-L1 is integral to the immune checkpoint pathway which is an important regulator of immune response. The interaction between PD-L1 and PD-1 provides a mechanism for immune tolerance which is part of the broader adaptive immune system pathway. PD-L1 is related to other immune checkpoint proteins such as CTLA-4 in its function to limit autoreactivity and promote immune homeostasis.
- Associated diseases and disorders
PD-L1 is most associated with cancer particularly in tumors such as melanoma and non-small cell lung cancer. PD-L1 expression on tumor cells often correlates with poor prognosis. PD-L1 directly interacts with PD-1 in these cancers a target for immunotherapies such as checkpoint inhibitors which aim to block this interaction and restore immune activity against tumors. PD-L1 involvement extends to autoimmune disorders where altered expression can impact tolerance and lead to immune-related tissue damage.
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1 product image
SDS-PAGE - Recombinant Cynomolgus monkey PD-L1 protein (Tagged) (Biotin) (ab271618)
SDS-PAGE analysis of 2 μg ab271618.
This protein runs at an apparent higher molecular weight due to glycosylation.
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