AB51121

Recombinant DnaK protein (Tag Free)

Name: Recombinant DnaK protein (Tag Free) (ab51121) | Abcam
Brand: Abcam Limited
SKU: AB51121

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(2 Publications)

Recombinant DnaK protein (Tag Free) is a Escherichia coli K-12 Full Length protein, in the 1 to 638 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

groP, grpF, seg, b0014, JW0013, dnaK, Chaperone protein DnaK, HSP70, Heat shock 70 kDa protein, Heat shock protein 70

1 Images

SDS-PAGE - Recombinant DnaK protein (Tag Free) (AB51121)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant DnaK protein (Tag Free) (AB51121)

15% SDS-PAGE

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE

applications

Biologically active

Accession

P0A6Y8

Animal free

Carrier free

Species

Escherichia coli K-12

Storage buffer

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Sequence info

[{"sequence":"MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQDGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAWVEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDIDDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLLDVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAADNKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLNEDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIESALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDDDVVDAEFEEVKDKK","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":638,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P0A6Y8","tags":[]}]

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

False

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Specifications

Form

Liquid

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General info

Function

Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the DnaA protein. Also participates actively in the response to hyperosmotic shock.

Sequence similarities

Belongs to the heat shock protein 70 family.

Post-translational modifications

Autophosphorylated; GrpE inhibits the autophosphorylation.

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information dnaK

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33858942

2021

Killed whole-genome reduced-bacteria surface-expressed coronavirus fusion peptide vaccines protect against disease in a porcine model.

Applications

Unspecified application

Species

Unspecified reactive species

Denicar Lina Nascimento Fabris Maeda,Debin Tian,Hanna Yu,Nakul Dar,Vignesh Rajasekaran,Sarah Meng,Hassan M Mahsoub,Harini Sooryanarain,Bo Wang,C Lynn Heffron,Anna Hassebroek,Tanya LeRoith,Xiang-Jin Meng,Steven L Zeichner

Molecular cell 70:614-627.e7 PubMed29754824

2018

CnoX Is a Chaperedoxin: A Holdase that Protects Its Substrates from Irreversible Oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Camille V Goemans,Didier Vertommen,Rym Agrebi,Jean-François Collet

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Recombinant DnaK protein (Tag Free)

SDS-PAGE - Recombinant DnaK protein (Tag Free) (AB51121)

Key facts

Purity

Expression system

Tags

Applications

Biologically active

Accession

Animal free

Carrier free

Species

Storage buffer

Reactivity data

Sequence info

Properties and storage information

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Specifications

Form

General info

Function

Sequence similarities

Post-translational modifications

Product protocols

Target data

Publications (2)

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Secondary Antibodies

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