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AB225607

Recombinant Dog Anionic Trypsin protein (His tag)

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Recombinant Dog Anionic Trypsin protein (His tag) is a Dog Full Length protein, in the 24 to 247 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

Anionic trypsin

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

6x His tag N-Terminus SUMO tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P06872

Animal free

No

Carrier free

No

Species

Dog

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Sequence info

[{"sequence":"IVGGYTCEENSVPYQVSLNAGYHFCGGSLISDQWVVSAAHCYKSRIQVRLGEYNIDVLEGNEQFINSAKVIRHPNYNSWILDNDIMLIKLSSPAVLNARVATISLPRACAAPGTQCLISGWGNTLSSGTNYPELLQCLDAPILTQAQCEASYPGQITENMICAGFLEGGKDSCQGDSGGPVVCNGELQGIVSWGYGCAQKNKPGVYTKVCNFVDWIQSTIAANS","proteinLength":"Full Length","predictedMolecularWeight":"40 kDa","actualMolecularWeight":null,"aminoAcidEnd":247,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P06872","tags":[{"tag":"6x His","terminus":"N-Terminus"},{"tag":"SUMO","terminus":"N-Terminus"}]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Anionic Trypsin also known as Trypsin-2 or PRSS2 is a serine protease with a molecular mass of approximately 25 kDa. It finds expression mainly in the pancreas. This protease functions by breaking down proteins into smaller peptides through cleaving peptide bonds at the carboxyl side of lysine and arginine residues. It plays an essential role in digestion being activated from its inactive precursor trypsinogen in the small intestine.
Biological function summary

Anionic trypsin participates in protein digestion within the digestive system. It is part of the pancreatic enzyme complex that includes other proteases like chymotrypsin and elastase. This complex activity ensures the effective breakdown of dietary proteins which is vital for nutrient absorption and utilization. Anionic trypsin further regulates itself through feedback inhibition managing its own activity to prevent damage to pancreatic tissues.

Pathways

Anionic trypsin plays a role in the proteolytic pathway important for nutrient assimilation. It operates alongside related enzymes such as chymotrypsin aiding the coordinated breakdown of proteins. Anionic trypsin is integral to the zymogen activation pathway where it converts trypsinogen to active trypsin ensuring digestive enzymes remain inactive until they reach the intestine where their function is most needed.

Anionic trypsin relates significantly to pancreatitis and cystic fibrosis. Mutations affecting its activity can lead to improper protein digestion contributing to inflammation in the pancreas as seen in pancreatitis. Anionic trypsin also interacts with CFTR protein influencing the pancreatic complications observed in cystic fibrosis. Understanding these interactions provides insights into treatment strategies for these conditions.

Specifications

Form

Liquid

General info

Sequence similarities

Belongs to the peptidase S1 family.

Product protocols

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