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AB314538

Recombinant Dog CD20 protein - Active (His tag)

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Recombinant Dog CD20 protein - Active (His tag) is a Dog Full Length protein, in the 1 to 297 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level, suitable for WB, FuncS.

View Alternative Names

CD20, MS4A1, B-lymphocyte antigen CD20, Membrane-spanning 4-domains subfamily A member 1

2 Images
Functional Studies - Recombinant Dog CD20 protein - Active (His tag) (AB314538)
  • FuncS

Supplier Data

Functional Studies - Recombinant Dog CD20 protein - Active (His tag) (AB314538)

Measured by its binding ability in a functional ELISA. Immobilized ab314538 at 10 μg/mL can bind an Anti-CD20 recombinant antibody, the EC50 is 147.3-199.0 ng/mL.

Western blot - Recombinant Dog CD20 protein - Active (His tag) (AB314538)
  • WB

Supplier Data

Western blot - Recombinant Dog CD20 protein - Active (His tag) (AB314538)

The protein has phosphorylation modification, which causes the WB detection result to be larger than the predicted value.

All lanes:

mouse anti-6*His monoclonal antibody

All lanes:

Western blot - Recombinant Dog CD20 protein - Active (His tag) (ab314538)

false

Key facts

Endotoxin level

< 1 EU/µg

Expression system

Mammalian

Tags

His tag C-Terminus

Applications

WB, FuncS

applications

Biologically active

Yes

Biological activity

Measured by its binding ability in a functional ELISA. Immobilized AB314538 at 10 μg/mL can bind an Anti-CD20 recombinant antibody, the EC50 is 147.3-199.0 ng/mL.

Accession

Q3C2E2

Animal free

Yes

Carrier free

No

Species

Dog

Reconstitution

Reconstitute in water

Storage buffer

pH: 7.4 - 8 Constituents: PBS, 6% Trehalose

storage-buffer

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Secondary Antibodies

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Primary Antibodies

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  • 5
  • 12
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC/-80degC. Our default final concentration of glycerol is 50%.
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Sequence info

[{"sequence":"MTTPRNSMSGTLPVDPMKSPTAMYPVQKIIPKRMPSVVGPTQNFFMRESKTLGAVQIMNGLFHIALGSLLMIHTDVCAPICITMWYPLWGGIMFIISGSLLAAADKNPRKSLVKGKMIMNSLSLFAAISGIIFLIMDIFNITISHFFKMENLNLIKAPMPYVDIHNCDPANPSEKNSLSIQYCGSIRSVFLGVFAVMLIFAFFQKLVTAGIVENEWKKLCSKPKSDVVVLLAAEEKKEQPIETTEEMVELTEIASQPKKEEDIEIIPVQEEEGELEINFAEPPQEQESSPIENDSIP","proteinLength":"Full Length","predictedMolecularWeight":"34.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":297,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Mammalian","accessionNumber":"Q3C2E2","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C|-80°C
Appropriate long-term storage conditions
-20°C|-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.
Biological function summary

CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.

Pathways

CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.

CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.
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Specifications

Form

Lyophilized

Additional notes

Purification technique - Centrifugation.

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General info

Function

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.

Sequence similarities

Belongs to the MS4A family.

Post-translational modifications

Phosphorylated. Might be functionally regulated by protein kinase(s) (By similarity).

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Product protocols

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Target data

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.
See full target information MS4A1
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