Recombinant Dog CD20 protein - Active (His tag) is a Full Length protein, in the 1 to 297 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level and suitable for WB, FuncS.
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Key facts
- Endotoxin level
< 1 EU/µg
- Expression system
Mammalian
- Tags
His tag C-Terminus
- Applications
WB, FuncS
- Biologically active
Yes
Amino acid sequence
M T T P R N S M S G T L P V D P M K S P T A M Y P V Q K I I P K R M P S V V G P T Q N F F M R E S K T L G A V Q I M N G L F H I A L G S L L M I H T D V C A P I C I T M W Y P L W G G I M F I I S G S L L A A A D K N P R K S L V K G K M I M N S L S L F A A I S G I I F L I M D I F N I T I S H F F K M E N L N L I K A P M P Y V D I H N C D P A N P S E K N S L S I Q Y C G S I R S V F L G V F A V M L I F A F F Q K L V T A G I V E N E W K K L C S K P K S D V V V L L A A E E K K E Q P I E T T E E M V E L T E I A S Q P K K E E D I E I I P V Q E E E G E L E I N F A E P P Q E Q E S S P I E N D S I P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
B-lymphocyte antigen CD20, Membrane-spanning 4-domains subfamily A member 1, CD20, MS4A1
Recommended products
Recombinant Dog CD20 protein - Active (His tag) is a Full Length protein, in the 1 to 297 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level and suitable for WB, FuncS.
Alternative names
B-lymphocyte antigen CD20, Membrane-spanning 4-domains subfamily A member 1, CD20, MS4A1
Key facts
- Endotoxin level
< 1 EU/µg
- Expression system
Mammalian
- Tags
His tag C-Terminus
- Applications
WB, FuncS
- Biologically active
Yes
- Biological activity
Measured by its binding ability in a functional ELISA. Immobilized AB314538 at 10 μg/mL can bind an Anti-CD20 recombinant antibody, the EC50 is 147.3-199.0 ng/mL.
- Accession
- Q3C2E2-1
- Animal free
Yes
- Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.14% PBS
Sequence info
Amino acid sequence
M T T P R N S M S G T L P V D P M K S P T A M Y P V Q K I I P K R M P S V V G P T Q N F F M R E S K T L G A V Q I M N G L F H I A L G S L L M I H T D V C A P I C I T M W Y P L W G G I M F I I S G S L L A A A D K N P R K S L V K G K M I M N S L S L F A A I S G I I F L I M D I F N I T I S H F F K M E N L N L I K A P M P Y V D I H N C D P A N P S E K N S L S I Q Y C G S I R S V F L G V F A V M L I F A F F Q K L V T A G I V E N E W K K L C S K P K S D V V V L L A A E E K K E Q P I E T T E E M V E L T E I A S Q P K K E E D I E I I P V Q E E E G E L E I N F A E P P Q E Q E S S P I E N D S I P
- Accession
- Protein length
Full Length
- Predicted molecular weight
34.4 kDa
- Amino acids
1 to 297
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Lyophilized
- Additional notes
Purification technique - Centrifugation.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C, -80°C
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC/-80degC. Our default final concentration of glycerol is 50%.
Supplementary info
- Activity summary
CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.
- Biological function summary
CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.
- Pathways
CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.
- Associated diseases and disorders
CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.
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2 product images
Western blot - Recombinant Dog CD20 protein - Active (His tag) (ab314538)
The protein has phosphorylation modification, which causes the WB detection result to be larger than the predicted value.
All lanes: mouse anti-6*His monoclonal antibody
All lanes: Western blot - Recombinant Dog CD20 protein - Active (His tag) (ab314538)
Functional Studies - Recombinant Dog CD20 protein - Active (His tag) (ab314538)
Measured by its binding ability in a functional ELISA. Immobilized ab314538 at 10 μg/mL can bind an Anti-CD20 recombinant antibody, the EC50 is 147.3-199.0 ng/mL.
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