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AB233676

Recombinant dog IL-4 protein (Active)

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Recombinant dog IL-4 protein (Active) is a Dog Full Length protein, in the 25 to 132 aa range, expressed in Escherichia coli, with >95%, <1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

Interleukin-4, IL-4, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1, IL4

2 Images
Functional Studies - Recombinant dog IL-4 protein (Active) (AB233676)
  • FuncS

Supplier Data

Functional Studies - Recombinant dog IL-4 protein (Active) (AB233676)

ab233676 induced proliferation of TF-1 cells.

SDS-PAGE - Recombinant dog IL-4 protein (Active) (AB233676)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant dog IL-4 protein (Active) (AB233676)

SDS-PAGE - Recombinant dog IL4 protein (Active) (ab233676) at 1 μg.

Lane 1 : Non-reducing conditions.

Lane 2 : Reducing conditions.

4-20% Tris-Glycine gel stained with Coomassie Blue.

Expected MW 12.9 kDa.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

TF-1 cell proliferation: ED50 ≤ 25 ng/mL (≥ 4.0 x 104 units/mg).

Accession

O77762

Animal free

Yes

Carrier free

No

Species

Dog

Reconstitution

Reconstitute at 0.1 mg/mL in water

Storage buffer

Constituents: 0.1% Trifluoroacetic acid

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Storage:

12 months from date of receipt when stored at -20°C to -80°C as supplied.

1 month when stored at 4°C after reconstituting as directed.

3 months when stored at -20°C to -80°C after reconstituting as directed.

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Sequence info

[{"sequence":"MHNFNITIKEIIKMLNILTARNDSCMELTVKDVFTAPKNTSDKEIFCRAATVLRQIYTHNCSNRYLRGLYRNLSSMANKTCSMNEIKKSTLKDFLERLKVIMQKKYYRH","proteinLength":"Full Length","predictedMolecularWeight":"12.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":132,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O77762","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
True
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Specifications

Form

Lyophilized

Additional notes

Produced using animal-free processes and contains only animal-free raw materials.

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General info

Function

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.

Sequence similarities

Belongs to the IL-4/IL-13 family.

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Product protocols

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Target data

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
See full target information IL4
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