Recombinant E. coli Acetate Kinase protein is a Escherichia coli K-12 Full Length protein, in the 1 to 400 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M S S K L V L V L N C G S S S L K F A I I D A V N G E E Y L S G L A E C F H L P E A R I K W K M D G N K Q E A A L G A G A A H S E A L N F I V N T I L A Q K P E L S A Q L T A I G H R I V H G G E K Y T S S V V I D E S V I Q G I K D A A S F A P L H N P A H L I G I E E A L K S F P Q L K D K N V A V F D T A F H Q T M P E E S Y L Y A L P Y N L Y K E H G I R R Y G A H G T S H F Y V T Q E A A K M L N K P V E E L N I I T C H L G N G G S V S A I R N G K C V D T S M G L T P L E G L V M G T R S G D I D P A I I F H L H D T L G M S V D A I N K L L T K E S G L L G L T E V T S D C R Y V E D N Y A T K E D A K R A M D V Y C H R L A K Y I G A Y T A L M D G R L D A V V F T G G I G E N A A M V R E L S L G K L G V L G F E V D H E R N L A A R F G K S G F I N K E G T R P A V V I P T N E E L V I A Q D A S R L T A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically. The main pathway for acetate production during exponential phase (PubMed:16080684).
Alternative names
ack, b2296, JW2293, ackA, Acetate kinase, Acetokinase
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Recombinant E. coli Acetate Kinase protein is a Escherichia coli K-12 Full Length protein, in the 1 to 400 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P0A6A3-1
- Animal free
- No
- Species
- Escherichia coli K-12
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M S S K L V L V L N C G S S S L K F A I I D A V N G E E Y L S G L A E C F H L P E A R I K W K M D G N K Q E A A L G A G A A H S E A L N F I V N T I L A Q K P E L S A Q L T A I G H R I V H G G E K Y T S S V V I D E S V I Q G I K D A A S F A P L H N P A H L I G I E E A L K S F P Q L K D K N V A V F D T A F H Q T M P E E S Y L Y A L P Y N L Y K E H G I R R Y G A H G T S H F Y V T Q E A A K M L N K P V E E L N I I T C H L G N G G S V S A I R N G K C V D T S M G L T P L E G L V M G T R S G D I D P A I I F H L H D T L G M S V D A I N K L L T K E S G L L G L T E V T S D C R Y V E D N Y A T K E D A K R A M D V Y C H R L A K Y I G A Y T A L M D G R L D A V V F T G G I G E N A A M V R E L S L G K L G V L G F E V D H E R N L A A R F G K S G F I N K E G T R P A V V I P T N E E L V I A Q D A S R L T A
- Protein length
- Full Length
- Predicted molecular weight
- 45.7 kDa
- Amino acids
- 1 to 400
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically. The main pathway for acetate production during exponential phase (PubMed:16080684).
- Sequence similarities
Belongs to the acetokinase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Acetate kinase sometimes referred to as ACK or ACKA is an enzyme that catalyzes the transfer of a phosphate group from ATP to acetate producing acetyl phosphate. This reaction is a reversible process. Acetate kinase has a molecular mass of approximately 43 kDa. It is expressed in various prokaryotic organisms including bacteria such as Escherichia coli. Additionally some eukaryotic organisms particularly certain fungi show expression of acetate kinase although with some differences in regulation and function compared to their prokaryotic counterparts.
- Biological function summary
Acetate kinase plays a role in bacterial energy metabolism. It assists in the regulation of the acetyl-CoA pool an important molecule in energy production pathways. Acetate kinase does not function as part of a larger enzyme complex; rather it operates independently. In prokaryotes it participates in energy conservation by converting acetyl phosphate into ATP via substrate-level phosphorylation therefore influencing cellular energy dynamics. The enzyme's activity directly impacts acetate metabolism and the balance between anabolism and catabolism within the cell.
- Pathways
Acetate kinase participates in the prokaryotic phosphotransacetylase-acetate kinase pathway which is integral to acetate metabolism and cellular respiration. This pathway collaborates closely with the TCA cycle by interacting with acetyl-CoA influencing the energy supply for cell processes. Proteins involved in related pathways include phosphotransacetylase which directly precedes acetate kinase in the pathway. Together they form a link between acetyl-CoA metabolism and energy generation impacting bacterial growth and survival.
- Associated diseases and disorders
Researchers have associated acetate kinase with conditions related to bacterial infections. In pathogenic bacteria such as Salmonella enterica acetate kinase contributes to virulence by adapting metabolic responses during infection aiding bacterial survival in host environments. Furthermore diseases linked to dysbiosis in the gut might be affected by aberrant bacterial metabolism involving acetate kinase as it alters microbial energy production. This relationship may involve dysregulated expression or activity of acetate kinase potentially impacting the overall balance of microbial populations and their interactions with host tissues.
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SDS-PAGE - Recombinant E. coli Acetate Kinase protein (ab187412)
15% SDS-PAGE analysis of ab187412 (3μg).
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