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AB235607

Recombinant E. coli Aquaporin Z protein (Active)

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Recombinant E. coli Aquaporin Z protein (Active) is a Escherichia coli K-12 Full Length protein, in the 1 to 231 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS.
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Functional Studies - Recombinant E. coli Aquaporin Z protein (Active) (AB235607)
  • FuncS

Supplier Data

Functional Studies - Recombinant E. coli Aquaporin Z protein (Active) (AB235607)

The binding of ab235607 with ytfE.

Immobilized ab235607 at 5 μg/ml can bind E.coli ytfE, the EC50 of E.coli ytfE protein is 197.90-259.70 μg/ml.

SDS-PAGE - Recombinant E. coli Aquaporin Z protein (Active) (AB235607)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant E. coli Aquaporin Z protein (Active) (AB235607)

ab235607 analyzed by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

6x His tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Measured by its binding ability in a functional ELISA. Immobilized ab235607 at 5 μg/mL can bind E.coli ytfE, the EC50 of E.coli ytfE protein is 197.90-259.70 μg/mL.

Accession

P60844-1

Animal free

No

Carrier free

No

Species

Escherichia coli K-12

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFGLTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD","proteinLength":"Full Length","predictedMolecularWeight":"27.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":231,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P60844","tags":[{"tag":"6x His","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aquaporin Z also known as AqpZ is a transmembrane protein that facilitates the rapid transport of water molecules across cell membranes. It has a molecular mass of approximately 27 kDa. Aquaporin Z is primarily expressed in bacterial cells where it plays an important role in maintaining osmotic balance. It contains a channel structure that allows selective permeability to water while preventing the passage of ions and other small molecules.
Biological function summary

Aquaporin Z functions to regulate water flow within bacterial cells which is essential for cell homeostasis and adaptation to environmental changes. It does not form part of a larger protein complex but operates independently within the cell membrane. This protein plays a significant role in processes such as nutrient absorption and waste removal in bacteria by regulating intracellular and extracellular water levels.

Pathways

Aquaporin Z is involved in osmoregulation and water transport pathways. These pathways ensure the cellular response to osmotic stress maintaining cell integrity. Aquaporin Z is related to other aquaporin proteins like Aquaporin 1 which perform similar water transport functions in different organisms. Together these proteins contribute to the larger framework of fluid balance regulation in living systems.

Aquaporin Z holds potential relevance in the context of certain infections or diseases affecting osmoregulation in bacteria potentially including disorders of water metabolism like cholera. As bacterial aquaporins might impact the pathogenesis of infections they could relate to proteins such as enterotoxins which exploit bacterial water channels to disrupt host water balance. Understanding the role of Aquaporin Z in these processes may aid in developing novel therapeutic strategies.
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General info

Function

Channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity.

Sequence similarities

Belongs to the MIP/aquaporin (TC 1.A.8) family.

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Product protocols

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Target data

Channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity.
See full target information aqpZ

Alternative Names

bniP, b0875, JW0859, aqpZ, Aquaporin Z, Bacterial nodulin-like intrinsic protein, Water channel AqpZ
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