Recombinant E. coli ATP-dependent Clp protease adapter protein ClpS (His tag)

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP-dependent Clp protease adapter protein ClpS also known as ClpS is a small protein with a mass of approximately 13 kDa. It operates within the Clp protease system and is part of the ClpS family. This protein plays a pivotal role in protein degradation by modulating the activity of ClpA within the Clp ATPase complex. ClpS is notably expressed in prokaryotic cells where it fine-tunes the specificity of substrates for proteolysis by directing them to the ClpAP protease complex.

Biological function summary

ATP-dependent Clp protease adapter protein ClpS guides substrate selection for degradation an essential part of maintaining cellular protein homeostasis. ClpS acts as an adapter ensuring only particular proteins are tagged for degradation by the ClpAP complex. By interacting with N-degron signals on substrate proteins ClpS serves as a quality control mechanism impacting essential cellular processes like stress response and protein turnover. The ClpAP complex itself which comprises ClpA and ClpP relies on ClpS for substrate specificity and efficiency.

Pathways

ATP-dependent Clp protease adapter protein ClpS is integral to the protein quality control pathways in bacteria. It features prominently in the N-end rule pathway where it recognizes proteins for degradation based on their N-terminal residues. ClpS binds selectively to proteins with N-degron sequences forming an important mediator with the ClpAP protease complex. Through this pathway ClpS exhibits functional interplay with proteins like ClpP which carries out the protease function ensuring the exhaustive removal of defective or misfolded proteins.

Malfunction or disruption of the ATP-dependent Clp protease adapter protein ClpS can impact bacterial virulence and adaptation. It links to disorders in bacteria associated with environmental stress survival as ClpS regulates protein degradation pathways significant for pathogen resilience. Additionally ClpS indirectly connects with other bacterial regulatory proteins critical to stress responses and survival positioning it as a potential target for antimicrobial strategies looking to combat bacterial infections through disruption of protein homeostasis mechanisms.