Recombinant E. coli Beta-lactamase protein is a Escherichia coli K-12 Full Length protein, in the 20 to 377 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A P Q Q I N D I V H R T I T P L I E Q Q K I P G M A V A V I Y Q G K P Y Y F T W G Y A D I A K K Q P V T Q Q T L F E L G S V S K T F T G V L G G D A I A R G E I K L S D P T T K Y W P E L T A K Q W N G I T L L H L A T Y T A G G L P L Q V P D E V K S S S D L L R F Y Q N W Q P A W A P G T Q R L Y A N S S I G L F G A L A V K P S G L S F E Q A M Q T R V F Q P L K L N H T W I N V P P A E E K N Y A W G Y R E G K A V H V S P G A L D A E A Y G V K S T I E D M A R W V Q S N L K P L D I N E K T L Q Q G I Q L A Q S R Y W Q T G D M Y Q G L G W E M L D W P V N P D S I I N G S D N K I A L A A R P V K A I T P P T P A V R A S W V H K T G A T G G F G S Y V A F I P E K E L G I V M L A N K N Y P N P A R V D A A W Q I L N A L Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Class C beta-lactamase which confers resistance to penicillins and cephalosporins (PubMed:12323371, PubMed:17956081, PubMed:23043117, PubMed:33199391, PubMed:6998377). Has benzylpenicillin- and cephaloridine-hydrolyzing activity (PubMed:3264154, PubMed:3264155, PubMed:6998377). Has weak cefuroxime, cefotaxime, cefoxitin and oxacillin-hydrolyzing activities (PubMed:19913034, PubMed:3264154, PubMed:3264155).
Additional Targets
Beta-lactamase
Alternative names
ampA, b4150, JW4111, ampC, Beta-lactamase, Cephalosporinase, CSase
Recommended products
Recombinant E. coli Beta-lactamase protein is a Escherichia coli K-12 Full Length protein, in the 20 to 377 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P00811-1
- Animal free
- No
- Species
- Escherichia coli K-12
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A P Q Q I N D I V H R T I T P L I E Q Q K I P G M A V A V I Y Q G K P Y Y F T W G Y A D I A K K Q P V T Q Q T L F E L G S V S K T F T G V L G G D A I A R G E I K L S D P T T K Y W P E L T A K Q W N G I T L L H L A T Y T A G G L P L Q V P D E V K S S S D L L R F Y Q N W Q P A W A P G T Q R L Y A N S S I G L F G A L A V K P S G L S F E Q A M Q T R V F Q P L K L N H T W I N V P P A E E K N Y A W G Y R E G K A V H V S P G A L D A E A Y G V K S T I E D M A R W V Q S N L K P L D I N E K T L Q Q G I Q L A Q S R Y W Q T G D M Y Q G L G W E M L D W P V N P D S I I N G S D N K I A L A A R P V K A I T P P T P A V R A S W V H K T G A T G G F G S Y V A F I P E K E L G I V M L A N K N Y P N P A R V D A A W Q I L N A L Q
- Accession
- P00811
- Protein length
- Full Length
- Predicted molecular weight
- 41.8 kDa
- Amino acids
- 20 to 377
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab104926 was purified using conventional chromatography.
General info
- Function
Class C beta-lactamase which confers resistance to penicillins and cephalosporins (PubMed:12323371, PubMed:17956081, PubMed:23043117, PubMed:33199391, PubMed:6998377). Has benzylpenicillin- and cephaloridine-hydrolyzing activity (PubMed:3264154, PubMed:3264155, PubMed:6998377). Has weak cefuroxime, cefotaxime, cefoxitin and oxacillin-hydrolyzing activities (PubMed:19913034, PubMed:3264154, PubMed:3264155).
- Sequence similarities
Belongs to the class-C beta-lactamase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta-lactamase often referred to as penicillinase is a protein that catalyzes the hydrolysis of beta-lactam antibiotics like penicillins. This enzyme breaks the beta-lactam ring structure rendering the antibiotic ineffective. Beta-lactamase proteins vary widely in their molecular mass with most commonly falling in the range of 29 to 35 kDa. These enzymes are predominantly expressed in bacteria including 'E. coli' where they confer antibiotic resistance. The production of beta-lactamase can occur at the periplasmic space in gram-negative bacteria or secreted in gram-positive bacteria.
- Biological function summary
Beta-lactamase enzymes cause antibiotic resistance which is a significant concern in treating bacterial infections. These proteins function as monomeric enzymes but may work with other factors or be part of larger resistance mechanisms. They degrade beta-lactam antibiotics protecting the bacteria from antibacterial agents. In 'E. coli' beta-lactamase production is upregulated in the presence of antibiotics showcasing an adaptive bacterial response.
- Pathways
Beta-lactamase activity integrates into bacterial defense mechanisms particularly through antibiotic resistance pathways. The enzyme plays an important role in the broader antibiotic resistance pathway working alongside other resistance proteins such as penicillin-binding proteins (PBPs) which alter the target sites of antibiotics. The production and function of these proteins ensure the survival of resistant bacteria even in antibiotic-rich environments.
- Associated diseases and disorders
Beta-lactamase enzymes are closely linked to challenging bacterial infections particularly in nosocomial (hospital-acquired) infections. Their presence complicates the treatment of diseases like pneumonia and urinary tract infections (UTIs) often making standard antibiotics ineffective. Beta-lactamase is associated with multidrug-resistant strains like extended-spectrum beta-lactamase (ESBL) producing bacteria which can complicate treatment options and outcomes. Understanding this protein and its interactions with other proteins like efflux pumps is important in addressing antibiotic resistance.
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SDS-PAGE - Recombinant E. coli Beta-lactamase protein (ab104926)
15% SDS-PAGE analysis of 3ug ab104926
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