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AB104926

Recombinant E. coli Beta-lactamase protein (His tag N-Terminus)

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Recombinant E. coli Beta-lactamase protein (His tag N-Terminus) is a Escherichia coli K-12 Full Length protein, in the 20 to 377 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

ampA, b4150, JW4111, ampC, Beta-lactamase, Cephalosporinase, CSase

1 Images
SDS-PAGE - Recombinant E. coli Beta-lactamase protein (His tag N-Terminus) (AB104926)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant E. coli Beta-lactamase protein (His tag N-Terminus) (AB104926)

15% SDS-PAGE analysis of 3ug ab104926

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P00811

Animal free

No

Carrier free

No

Species

Escherichia coli K-12

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAPQQINDIVHRTITPLIEQQKIPGMAVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLSDPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAPGTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWGYREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYWQTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGATGGFGSYVAFIPEKELGIVMLANKNYPNPARVDAAWQILNALQ","proteinLength":"Full Length","predictedMolecularWeight":"41.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":377,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P00811","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-lactamase often referred to as penicillinase is a protein that catalyzes the hydrolysis of beta-lactam antibiotics like penicillins. This enzyme breaks the beta-lactam ring structure rendering the antibiotic ineffective. Beta-lactamase proteins vary widely in their molecular mass with most commonly falling in the range of 29 to 35 kDa. These enzymes are predominantly expressed in bacteria including 'E. coli' where they confer antibiotic resistance. The production of beta-lactamase can occur at the periplasmic space in gram-negative bacteria or secreted in gram-positive bacteria.
Biological function summary

Beta-lactamase enzymes cause antibiotic resistance which is a significant concern in treating bacterial infections. These proteins function as monomeric enzymes but may work with other factors or be part of larger resistance mechanisms. They degrade beta-lactam antibiotics protecting the bacteria from antibacterial agents. In 'E. coli' beta-lactamase production is upregulated in the presence of antibiotics showcasing an adaptive bacterial response.

Pathways

Beta-lactamase activity integrates into bacterial defense mechanisms particularly through antibiotic resistance pathways. The enzyme plays an important role in the broader antibiotic resistance pathway working alongside other resistance proteins such as penicillin-binding proteins (PBPs) which alter the target sites of antibiotics. The production and function of these proteins ensure the survival of resistant bacteria even in antibiotic-rich environments.

Beta-lactamase enzymes are closely linked to challenging bacterial infections particularly in nosocomial (hospital-acquired) infections. Their presence complicates the treatment of diseases like pneumonia and urinary tract infections (UTIs) often making standard antibiotics ineffective. Beta-lactamase is associated with multidrug-resistant strains like extended-spectrum beta-lactamase (ESBL) producing bacteria which can complicate treatment options and outcomes. Understanding this protein and its interactions with other proteins like efflux pumps is important in addressing antibiotic resistance.
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Specifications

Form

Liquid

Additional notes

ab104926 was purified using conventional chromatography.

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General info

Function

Class C beta-lactamase which confers resistance to penicillins and cephalosporins (PubMed : 12323371, PubMed : 17956081, PubMed : 23043117, PubMed : 33199391, PubMed : 6998377). Has benzylpenicillin- and cephaloridine-hydrolyzing activity (PubMed : 3264154, PubMed : 3264155, PubMed : 6998377). Has weak cefuroxime, cefotaxime, cefoxitin and oxacillin-hydrolyzing activities (PubMed : 19913034, PubMed : 3264154, PubMed : 3264155).

Sequence similarities

Belongs to the class-C beta-lactamase family.

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Product protocols

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Target data

Class C beta-lactamase which confers resistance to penicillins and cephalosporins (PubMed : 12323371, PubMed : 17956081, PubMed : 23043117, PubMed : 33199391, PubMed : 6998377). Has benzylpenicillin- and cephaloridine-hydrolyzing activity (PubMed : 3264154, PubMed : 3264155, PubMed : 6998377). Has weak cefuroxime, cefotaxime, cefoxitin and oxacillin-hydrolyzing activities (PubMed : 19913034, PubMed : 3264154, PubMed : 3264155).
See full target information ampC
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